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Research ArticleOpen accessEvery word in the titleGenetic State of Pulsatilla Patens (L.) Mill. Coenopopulations Based on DNA LabelingRustem Saltanovich Beishov, Gulnar Sultangazina, Indira Saltanovna Beishova et al.In the article, the results of studying the gene pools of the Kazakhstan coenopopulations of a rare plant species Pulsatilla patens (L.) Mill. are shown. The biomaterial taken from five coenopopulations located in Northern Kazakhstan has been used as samples. To identify the multilocus DNA polymorphism, the authors have used ISSR (Inter Simple Sequence Repeats, or intermicrosatellite analysis) labeling. The … …OnLine Journal of Biological Sciences, Vol. 21, Issue 1 · 2 Apr 2021 · 5,944 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleMeasurement of the Gene Expression and Polymorphisms of c-myc and p53 Genes in HBV Infected PatientsFayed Koutb, Salah Abdel-Rahman, Ehab Hassouna, Amany HaggagThis study explained the c-myc and p53 gene expression and polymorphisms in chronic (40), cirrhosis (30) and Hepatocellular Carcinoma, (HCC) (30) patients related to Hepatitis B Virus (HBV) infection and healthy control (50) in Egypt. Where, c-myc (intron 8) and p53 (codon 72) gene expression and polymorphisms were determined using qRT-PCR and PCR-RFLP techniques. The results showed that c-myc gene … …OnLine Journal of Biological Sciences, Vol. 21, Issue 1 · 22 Feb 2021 · 7,229 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleComparative Effect of Ulvan and Biosynthesized Silver Nanoparticles on Different Cell Lines Cytotoxicity and Gene ExpressionHadear Hanie Amin, Mohammed Ahmed Taha AbdelreheemUntil now, researchers searching for compounds that contribute for treating the most serious disease of this age, cancer. Researchers hope to contribute to this through this research. Activities of both Ulvan extracted from Ulva lactuca and silver nanoparticles (Ag-NPs) were examined against seven different cancer cell types called (Caco2 cells, A549 cells, Mcf7 cells, Hep2 cells, HepG2 cells, PC3 cells, … …OnLine Journal of Biological Sciences, Vol. 20, Issue 2 · 30 Jun 2020 · 6,675 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleScreening of Blast Resistance Genes from South Tapanuli Rice Cultivars, North Sumatra, IndonesiaSaleha Hannum, Hesti Wahyuningsih, Riyanto Sinaga et al.Rice blast disease caused by a phytopathogenic fungus, Magnaporthe oryzae, has been considered as a major threat to global rice productivity, including Indonesia. South Tapanuli is a regency in North Sumatra, known for its intensive and significant rice production activities. A collection of 13 rice cultivars originating from the region, is claimed for its resistances against the rice blast disease … …OnLine Journal of Biological Sciences, Vol. 20, Issue 2 · 25 May 2020 · 7,537 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleGenetics Polymorphisms of INHA in Local Goat Populations in South-West Sulawesi Region of IndonesiaMuhammad Ihsan Andi Dagong, Sri Rachma Aprilita Bugiwati, Lellah Rahim, Nurul PurnomoInhibin subunit alpha (INHA) is one important gene that control reproductive characteristics of animal. The aim of this research was to identify the genetic diversity of INHA gene of Kacang and Peranakan Etawa (PE) goat as local goat breed which were maintained by farmers at goat development centers in Sulawesi region, Indonesia. DNA samples were collected from 137 heads Kacang … …American Journal of Animal and Veterinary Sciences, Vol. 15, Issue 1 · 12 Mar 2020 · 7,273 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEvaluation of Polymorphisms at Heat Shock Protein 90 Gene by High Resolution Melting Assays for Potential Heat Tolerance among Nigerian Zebu Cattle BreedsGbolabo Onasanya, George Msalya, Aranganoor Thiruvenkadan et al.Heat Shock Protein (HSP) 90 gene is a member of HSPs sub-family that act as molecular chaperons whenever animals come under thermal stress. The genes fulfill essential roles of providing cellular protection, immune response, protein synthesis, protein folding and unfolding, protection from cellular stress, inhibitory apoptosis and adaptation. This study was designed to analyze polymorphisms of HSP 90 and to … …American Journal of Animal and Veterinary Sciences, Vol. 15, Issue 1 · 7 Mar 2020 · 7,258 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleComparison of IncL/M Plasmids Using the Neighbor-Joining Method on Basis repA and excA GenesTouati Abdelaziz, Mairi AssiaThe aim of this study is to make comparison of IncL/M groups on basis of two genes candidates’ repA and excA genes. The sequences of 27 plasmids were compared using the neighbor-joining method. This method was used to construct a phylogenetic tree for the nucleotide sequences of two genes (repA and excA), using the program MEGA X software. The evolutionary … …Current Research in Bioinformatics, Vol. 8, Issue 1 · 27 Feb 2020 · 7,088 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleForensic Relationship between ATP6 Gene and Sperm Motility in a Saudi PopulationSafa A.M. Elsanousi, Murid Javed, Hamad Sufyan, Sayed A.M. AmerApproximately 600 bp of ATP synthase subunit 6 mitochondrial gene (ATP6) were amplified and sequenced for 110 semen samples collected from infertile and normal Saudi men inhabiting Riyadh province. These data were analyzed in order to identify the Single Nucleotide Polymorphisms (SNPs) associated with male infertility. Six silent substitutions G8697A, G8856A, T8889C, T8952C, C9075T and C9060A were recorded with one … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 1 · 11 Jan 2020 · 7,617 accessesView PDFCite
InvestigationOpen accessEvery word in the titleGenotypic Characterization of Antimicrobial Resistance-Associated Genes in Citrobacter Freundii Isolated from Patients with Urinary Tract Infection in Al-Najaf Governorate-IraqThualfakar Hayder, Ahmed Abduljabbar Jaloob AljanabyCitrobacter freundii is one of the most important pathogens that cause urinary tract infection in human because it harbors many types of antimicrobials resistance-associated genes. Therefore, this study aimed to evaluate the prevalence of antimicrobial resistance-associated genes citrobacter freundii isolated from patients with urinary tract infection in Al-Najaf governorate-Iraq. A total of 30 strains of citrobacter freundii isolated from urine … …OnLine Journal of Biological Sciences, Vol. 19, Issue 2 · 26 Jun 2019 · 8,541 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleComparative Functional Analysis of the Basic Helix-Loop-Helix Proteins in the Clawed Frogs' Genomes With Common Essential Pathways and Enriched Gene Ontology TermsWuyi LiuThe international Gene Ontology (GO) and pathway databases were used to functionally analyze the clawed frogs' Basic Helix-Loop-Helix (bHLH) transcription factors of Xenopus tropicalis and Xenopus laevis in a updated genome-wide survey. There were 41 GO terms and one pathway significantly enriched for Xenopus tropicalis, whereas there were 45 GO terms and 3 pathways significantly enriched for Xenopus laevis. Among … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 1 · 28 Mar 2019 · 8,490 accessesView PDFCite