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419 results for “enzyme”
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Research ArticleOpen accessEvery word in the titleEnhanced Fatty Acid Production in Escherichia coli by Over-Expression of NADPH Generating EnzymesYuanda Song, Yong Q. Chen, Haiqin Chen et al.NADPH is an essential factor for Fatty Acid (FA) biosynthesis. The effect of NADPH generating enzymes [NADP+-dependent malic enzyme (NADP-ME), NADP+-dependent isocitrate dehydrogenase (NADP-IDH), Glucose 6-Phosphate Dehydrogenase (GPD) and 6-Phosphogluconate Dehydrogenase (PGD)] on FA biosynthesis was investigated in an engineered Escherichia coli BL21 △fadE/pTE. Among NADPH-generating enzymes, GPD and ICD increased total FA production by 60 and 16% respectively. Especially … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 23 Oct 2017 · 8,578 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleOptimisation of Bromelain Enzyme Extraction from Pineapple (Ananas comosus) and Application in Process IndustryResmi Mohan, Venkatasubramanian Sivakumar, Thirumalaisamy Rangasamy, Chellappa MuralidharanBromelain is a general name for a family of sulfhydryl proteolytic enzymes obtained from Ananas comosus, the pineapple plant. The objective of the present study is to compare the activity of Bromelain from pineapple fruit and peel and to check the efficiency of this crude Bromelain enzyme in unhairing step in leather processing. The crude Bromelain was extracted and activity … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 3 · 19 Sep 2016 · 12,650 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleComparison of enzyme Immunoassay with Immunofluorescence Assay for the Diagnosis of Mycoplasma pneumoniae Respiratory Tract Infections in ChildrenOsama Mohammed Saed Abdul-WahabMycoplasma pneumoniae is a frequent cause of acute respiratory infections in both children and adults. Currently, diagnosis of M. pneumoniae infection will based principally on serology and the detection of IgM can provide an early and sensitive diagnosis in children. A variety of commercial immunodiagnostic assays, such as Indirect Enzyme-Linked Immunosorbent (ELISA) assay and Indirect Immunofluorescence (IFA), are now available … …American Journal of Immunology, Vol. 9, Issue 2 · 21 Jun 2013 · 7,412 accessesView PDFCite
Research ArticleOpen accessEvery word in the titlePotential Applications of Chitosan Nanoparticles as Novel Support in Enzyme ImmobilizationHoda Jafarizadeh Malmiri, Mohammad Ali Ghaz Jahanian, Aydin BerenjianChitosan is an attractive natural biopolymer from renewable resources with the presence of reactive amino and hydroxyl functional groups in its structure. Due to the good biocompatibility of chitosan, it can be used in magnetic-field assisted drug delivery, enzyme or cell immobilization and many other industrial applications. In the past decade, nanotechnology has been a considerable research interest in the … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 4 · 25 Dec 2012 · 11,302 accessesView PDFCite
Research ArticleOpen accessEvery word in the titlePapain, a Plant Enzyme of Biological Importance: A ReviewFlorence Mamboya, Ezekiel AmriPapain is a plant proteolytic enzyme for the cysteine proteinase family cysteine protease enzyme in which enormous progress has been made to understand its functions. Papain is found naturally in papaya (Carica papaya L.) manufactured from the latex of raw papaya fruits. The enzyme is able to break down organic molecules made of amino acids, known as polypeptides and thus … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 2 · 22 Jun 2012 · 44,698 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleExtraction and Purification of Collagenase Enzymes: A Critical ReviewSaid M Daboor, Suzanne M Budge, Abdel E Ghaly et al.Problem statement: Enzymes have vital roles in several industrial processes (foods, cosmetics, nutraceuticals and pharmaceuticals) due to their highly selective nature and high activity at very low concentrations. Recent efforts to identify new sources of useful enzymes have been concentrated on the marine environment because of the potential to make use of processing wastes. About 35-50% of the mass of … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 14,311 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEffects of Enzyme Supplementation on Performance, Carcass characteristics, Carcass Composition and Some Blood Parameters of Broiler ChickenH. HajatiProblem statement: Endogenous enzymes of broilers cannot adequately digest nonstarch polysaccharides and subsequently the ingestion of high levels of soluble NSP leads to increased digesta viscosity and reduced nutrient digestibility and absorption. Supplementation of NSPs degrading enzymes may remove the anti nutritive effects of NSPs and release some nutrients (starch, protein) from these elements. Therefore, in a completely randomized design … …American Journal of Animal and Veterinary Sciences, Vol. 5, Issue 3 · 30 Sep 2010 · 9,649 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleTailor-Made Enzyme Carriers: Preparation and Use of Adsorbents Specifically Designed to Immobilize Allosteric Enzymes in Activated ConformationZahra SalemiProblem statement: The enzyme immobilization has experienced substantial growth in the recent past and an ever increasing amount of study has been reported on various aspects of immobilized enzymes. In most of these investigations, catalytic activities are found to be diminished as compared to the enzyme free in solution. Approach: Hydrophobic adsorbents were prepared containing L-leucine or citric acid, two … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 2 · 30 Jun 2010 · 7,558 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleOn the Hormetic Behaviour of Drugs Binding to Different Redox States of Amine Oxidase EnzymesDeepak Narang, G. Reid McDonald, David J. Smith et al.Classical monoamine oxidases and copper-containing semicarbazide-sensitive amine oxidases are drug targets for a variety of established and novel pharmaceuticals used to treat conditions such as depression, Parkinsonism and inflammatory disorders. Development of enzyme inhibitors, both historically and currently, assumes an underlying adherence of these enzymes to Michaelis-Menten kinetic principles. In this mini-review, we discuss historical data from several laboratories and … …American Journal of Pharmacology and Toxicology, Vol. 3, Issue 1 · 31 Mar 2008 · 6,928 accessesView PDFCite
Research ArticleOpen accessEvery word in the titlePurification of A Recombinant Thrombin-like Enzyme, Gloshedobin by Egg Yolk Antibody-Coupled AdsorbentsQing Yang, Xuyu Lei, Jianqiang Xu, Lijia AnThe gloshedobin, a snake venom thrombin-like enzyme was biosynthesized in the soluble form and purified by egg yolk antibody-coupled adsorbents from E. coli. As His-tag is not favored from the point of view of high-level and soluble expression, we herein constructed a recombinant gloshedobin without His-tag and developed a novel egg yolk antibody (IgY)-immunoaffinity chromatography for its purification in a … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 1 · 31 Mar 2005 · 6,690 accessesView PDFCite