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18 articles for “Trypsin”
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Research ArticleOpen accessIn the abstractCharacterization of Crude Polyphenol Oxidase Activity in Buckwheat SproutsYuedong Song, Yangfang Zhang, Yilin Wang et al.Buckwheat sprouts can not only effectively reduce the trypsin inhibitor in buckwheat grain, but also improve the content of bioactive ingredients such as flavonoids and other effective components. However, Polyphenol Oxidase (PPO) in buckwheat sprouts causes browning of food, which limits the application of buckwheat sprouts in food. In addressing this problem, studying the characteristics of PPO in buckwheat sprouts … …American Journal of Biochemistry and Biotechnology, Vol. 17, Issue 2 · 25 Jun 2021 · 6,486 accessesView PDFCite
Research ArticleOpen accessIn the abstractOptimization of Hydrolysis Conditions for Obtaining Antithrombotic Peptides from Tenebrio Molitor LarvaeFangyuan Chen, Han Jiang, Yini Gan et al.Tenebrio molitor larvae was hydrolyzed by pepsin and trypsin for preparing high antithrombotic activity hydrolysate. The significant variables were selected according to the Plackett-Burman design and further optimized by the Response Surface Methodology (RSM). Four factors, including substrate concentration, pepsin digestion time, pepsin amount and trypsin amount, respectively, influenced significantly on the hydrolysis effectiveness. The best hydrolysis conditions obtained using … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 2 · 20 May 2019 · 8,722 accessesView PDFCite
Research ArticleOpen accessIn the abstractOptimization of Hydrolysis Conditions for the Production of Iron-Binding Peptides from Scad (Decapterus maruadsi) Processing ByproductsWenting Zhang, Yanan Li, Jie Zhang, Guangrong HuangThe scad (Decapterus maruadsi) processing byproduct (SPB) was hydrolyzed by four commercial enzymes, namely, trypsin, flavourzyme, protamex and alcalase, for preparing high Iron-Binding Capacity (IBC) hydrolysate. Alcalase was the best choice for obtaining high IBC hydrolysate from SPB. Response surface methodology using a central-composite design was employed to optimize the enzymatic hydrolysis conditions with alcalase to obtain a maximum hydrolysate … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 4 · 17 Oct 2016 · 7,589 accessesView PDFCite
Research ArticleOpen accessIn the abstractIsolation and Identification of Staphylococcus epidermidis S14 Screening Extracellular Antimicrobial MetabolitesJie Zhang, Han Jiang, Jiaxin Jiang, Guangrong HuangTotal 24 isolates with antimicrobial activity against Staphylococcus aureus, Escherichia coli, Bacillus cereus and Shigella castellani, out of 259 strains, were isolated from the internal of seven marine fishes. The extracellular metabolites of strain S14, isolated from the internal of shark, showed stronger inhibition against the four tested bacteria, including G- and G+ bacteria. Based on the 16S rDNA the … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 1 · 19 Mar 2016 · 7,567 accessesView PDFCite
Research ArticleOpen accessIn the abstractMORPHOPHYSIOLOGICAL ADAPTATIONS OF THE GASTROINTESTINAL TRACT IN PIGLETS FED A SESAME MEAL OR SOYBEAN MEAL DIETAraceli Aguilera Barreyro, Tércia Reis de Souza, Gerardo Mariscal-Landín et al.An important indicator to recommend a protein source for piglet nutrition is the absence of intestinal damage. The effects of sesame or soybean meal based diets on Gastrointestinal Tract (GIT) morphophysiology, diarrhea incidence and severity in piglets were studied during the first two weeks post-weaning. Thirty-six piglets weaned at 21 days of age were fed one of three diets: A … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 1 · 11 Jan 2014 · 7,892 accessesView PDFCite
Research ArticleOpen accessIn the abstractBIOCHEMICAL STUDIES ON NIGERIAN MONODORA TENUIFOLIA SEEDEkeanyanwu Raphael Chukwuma, Njoku Obioma UzomaThe nutritive constituents of the seeds of Monodora tenuifolia were analyzed to augment the available information on Monodora tenuifolia research. Blood glucose and lipid profile were investigated on the flavonoid rich fraction of M. tenuifolia in rats. The composition (gkg-1) of alkaloids, cyanogenic glycosides, tannins and flavonoids were 13.3±0.1, 21.2×10-2±0.6, 1.3±0.1, 1.7±0.1 and 11.7±1.1 respectively. The proximate composition (gkg-1) of … …American Journal of Agricultural and Biological Sciences, Vol. 8, Issue 4 · 30 Sep 2013 · 7,572 accessesView PDFCite
Research ArticleOpen accessIn the abstractAntimicrobial Activity Studies of Bactoriocin Produced by Lactobacilli Isolates from Carrot KanjiHarshada M. Sowani, Prakash ThoratProblem statement: In the present study, Staphylococcus aureus a causative agent of food poisoning is selected as a test organism to study the antimicrobial effect of bacteriocin. S. aureus produces number of exotoxins and enterotoxins which enters the body via contaminated food causing illness. Approach: In this case the use of antibiotics is one of the ways of treatment, but … …OnLine Journal of Biological Sciences, Vol. 12, Issue 1 · 2 Mar 2012 · 8,543 accessesView PDFCite
Research ArticleOpen accessIn the abstractExtraction and Purification of Collagenase Enzymes: A Critical ReviewSaid M Daboor, Suzanne M Budge, Abdel E Ghaly et al.Problem statement: Enzymes have vital roles in several industrial processes (foods, cosmetics, nutraceuticals and pharmaceuticals) due to their highly selective nature and high activity at very low concentrations. Recent efforts to identify new sources of useful enzymes have been concentrated on the marine environment because of the potential to make use of processing wastes. About 35-50% of the mass of … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 14,311 accessesView PDFCite