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228 results for “Substrate”
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Research ArticleOpen accessEvery word in the titleStructural and Substrate Interaction Properties of Alkaline Phosphatase from Paenibacillus sp. PSB04: In-Silico AnalysisHerman Umbau Lindang, Cahyo BudimanA Phosphate-Solubilising Bacterium (PSB) of Paenibacillus sp. PSB04 was previously isolated from the Sabah tropical rainforest in Malaysia. Interestingly, the genome sequence of the PSB04 strain harbored an Alkaline Phosphatase (AP) (EC 3.1.3.1) gene and was hypothesized to have unique structural characteristics. Therefore, this study aims to determine the AP three-Dimensional (3D) model and catalytic mechanism from Paenibacillus sp. PSB04 … …OnLine Journal of Biological Sciences, Vol. 22, Issue 3 · 16 Jul 2022 · 5,910 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleAdsorption Behavior of Bone Morphogenetic Protein-2 on a Graphite Substrate for Biomedical ApplicationsIzabele Marquetti, Salil DesaiThe interaction of proteins with biomaterials plays an important role for several biomedical applications including implants and tissue engineering. Although different experimental approaches have been explored, the investigation of the molecular models for protein-substrate interaction is warranted to control of cellular functions. In this study, Molecular Dynamics (MD) simulations were used to study the adsorption behavior of Bone Morphogenetic Protein-2 … …American Journal of Engineering and Applied Sciences, Vol. 11, Issue 2 · 19 Jun 2018 · 8,705 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleUtilization of Banana, Pineapple and Watermelon Wastes-Substrate: As Consortiums to Remediating Cyanide Polluted SoilFlorence Obiageli Nduka, Samuel Chibuike Ubani, Victor Eshu Okpashi et al.The act of processing cassava for varied food products has come with unguided and wide-spread contamination of cyanide in the environment. The need for effective reduction in cost to enable remediation of cyanide-contaminated sites from cassava mill factories is the purpose of this investigation. This involves bio-stimulation of organic wastes with indigenous microorganisms to degrade cyanide. The application of organic … …American Journal of Environmental Sciences, Vol. 14, Issue 2 · 30 Apr 2018 · 9,930 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleProbing the Substrate Specificity and Intersubunit Interactions of Brevundimonas Diminuta Glutaryl Acylase with Site-Directed MutagenesisMichael Eldarov, Anna Lyashenko, Tatyana Sherbakova et al.Glutaryl-7-ACA acylases (GLA) are industrial enzymes widely used for the production of 7-aminocepahlosporanic acid (7-ACA)-the starting compound for manufacturing of semisynthetic cephalosporin antibiotics. Generation of mutant GLA's with increased activity and stability, capability for single-step conversion of cephalosporin C (CPC) directly to 7-ACA is a promising route to improve the current biocatalytic technologies. In this study GLA from Brevundimonas diminuta … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 3 · 12 Nov 2014 · 8,160 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleAmelioration of Inducible Nitric Oxide Synthase, Insulin like Growth Factor-1 Gene Expression and Insulin Receptor Substrate-1 in Liver Tissue of Insulin Resistant Rats Treated With L-CarnitineHala M. GhanemProblem statement: It has been reported that genes expression (inducible Nitric Oxide (iNOS). Insulin-like Growth Factor-1(IGF-1) have a role in both glucose homeostasis and insulin resistance. The aim of this study was designed to evaluate the amelioration of the iNOS, IGF-1 genes expression as well as IRS-1 in liver tissues of rats fed high fructose diet treated with Lcarnitine. Approach: … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 3 · 30 Sep 2010 · 7,264 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleModulation of Insulin Receptor Substrate-1 and Some Inflammatory Variables in Hyperinsulinemic Rats Treated with Cinnamon ExtractMohamed H. Mahfouz, Hala M. Ghanem, Mona A. MohamedCinnamon Extract (CE) has shown to be generally safe when ingested and to have many pharmacological properties. Problem statement: Study the effects of daily intake of CE on the modulation of hepatic, cardiac Insulin Receptor Substrate-1 (IRS-1) and their relations to some inflammatory variables in hyperinsulinemic rats. Approach: The influence of CE administered orally was studied in hyperinsulinemic rats. Eighteen … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 1 · 31 Mar 2010 · 7,810 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleCharacterization of Substrate-Borne Vibrational Signals of Euschistus servus (Heteroptera: Pentatomidae)Brittany Lampson, Young Han, Ahmad Khalilian et al.Problem Statement: Stink bugs were not major pests of cotton in the southeastern United States until cotton varieties containing Bacillus thuringiensis (Bt) transgenes came into common use, enabling reductions in insecticide applications against highly destructive lepidopteran pests. To maintain the benefits of reduced insecticide applications, it would be advantageous to detect and target isolated populations of stink bugs before they … …American Journal of Agricultural and Biological Sciences, Vol. 5, Issue 1 · 31 Mar 2010 · 8,272 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEffect of Delaying Sporulation by Addition of Ammonium Sulphate on the Fermentation of Palm Kernal Cake Based Substrate by Aspergillus nigerK. H. Swe, A. R. Alimon, M. RaminProblem statement: Asexual sporulation is a common reproductive mode for a diverse group of fungi and the ultimate limiting factor in fungal biomass production. Approach: Delaying sporulation may increase the biomass in terms of mycelial production and the same time increase production of enzymes. Results: In order to delay the sporulation different levels of ammonium sulphate (10, 12 and 14%) … …American Journal of Agricultural and Biological Sciences, Vol. 4, Issue 4 · 31 Dec 2009 · 8,399 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleAtomic Layer Epitaxial Growth of Gaas on Porous Silicon SubstrateMohamed Lajnef, Afrah Bardaoui, Isabelle Sagne et al.GaAs thin film has been grown on porous silicon by metal organic chemical vapour deposition (MOCVD) for different growth temperatures using atomic layer epitaxy (ALE) technique. The morphology of GaAs layer was investigated by atomic force microscopy (AFM). The effect of growth temperature is studied using photoluminescence measurements (PL).The photoluminescence spectra revealed a dissymmetry form toward high energies attributed to … …American Journal of Applied Sciences, Vol. 5, Issue 5 · 31 May 2008 · 7,027 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleProduction of Cellulase from Oil Palm Biomass as Substrate by Solid State BioconversionMd. Z. Alam, Nurdina Muhammad, Mohd E. MahmatSolid state bioconversion (SSB) of lignocellulosic material oil palm biomass (OPB) generated from palm oil industries as waste was conducted by evaluating the enzyme production through filamentous fungus in lab-scale experiment. OPB in the form of empty fruit bunches (EFB) was used as the solid substrate and treated with the fungus Trichoderma harzianum to produce cellulase. The results presented in … …American Journal of Applied Sciences, Vol. 2, Issue 2 · 28 Feb 2005 · 7,440 accessesView PDFCite