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Research ArticleOpen accessIn the abstractAnalysis of the Utility of MPT64, Drug Susceptibility Testing and Tuberculosis/Nontuberculous Mycobacteria Reverse Transcription Polymerase Chain Reaction for Tuberculosis Diagnosis and Efficiency Compared with Traditional CultureHo Keun Choi, Ga Yeon Kim, Tae Soung Kim, Jae Kyung KimDefining the molecular mechanisms underlying the dormant state of Mycobacterium tuberculosis (MTB) is essential for developing more effective treatments for tuberculosis (TB) infection. We investigated the positivity and resistance rates of TB tests on 6,174 samples referred to a single hospital. Of these, 342 cases (5.5%) were confirmed as positive by acid-fast bacilli staining, 243 cases (71.1%) were detected as … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 4 · 5 Dec 2024 · 4,206 accessesView PDFCite
Research ArticleOpen accessIn the abstractCoupling Analysis of Thermodynamic Equilibrium of Mass Transfer and Microbial Community Succession in Multiphase System of Dark Tea Solid-State FermentationZhanjun Liu, Jiawei Sun, Taotao Li et al.During the solid-state fermentation of dark tea, the limited material migration, the dynamic evolution of the thermodynamic equilibrium state, and the highly dynamic succession of the microbial community make it difficult to analyze the quality formation mechanism systematically. Traditional research has focused on microbial diversity or sensory quality analysis, lacking a systematic discussion on the regulatory effects of material migration … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 3 · 10 Aug 2026 · 1,004 accessesView PDFCite
Research ArticleOpen accessIn the abstractEstablishment and Application of Rapid Quantitative Detection Technology for Bacillus cereusFeilong Sun, Hanyu Li, Jingxue Qian et al.Infections caused by Bacillus cereus often occur, causing a certain degree of health hazards and economic losses to people around the world. Rapid quantitative detection is of great significance for prevention. This study aims to develop a simple and rapid quantitative detection method for B. cereus, which can be used to monitor the contamination of B. cereus in food samples. … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 1 · 21 Mar 2023 · 5,564 accessesView PDFCite
Research ArticleOpen accessIn the abstractPreparation, Characterization and Antioxidant Activity of a Novel Acetylated Polysaccharide from Flammulina VelutipesYingyun Peng, Han Jiang, Yufeng Wu et al.In this study, a novel polysaccharide FVP2a wasextracted and purified from F. velutipes. Ac-FVP2a was prepared bychemical acetylation. The preparation process of Ac-FVP2a was optimized usingthe response surface method based on three experimental factors includingreaction time, reaction temperature, and the ratio of FVP2a to aceticanhydride. The physicochemical characteristics andantioxidant activities of Ac-FVP2a in vitro were investigated. The results showed that … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 3 · 20 Sep 2022 · 5,207 accessesView PDFCite
Research ArticleOpen accessIn the abstractPhosphorylation, Characterization, and Antioxidant Activity of Polysaccharides from Flammulina Velutipes ScrapsYingyun Peng, Xue Bai, Hanwen Xu et al.In this study, polysaccharides from Flammulina velutipes Scraps(FVSP) were extracted. The preparation conditions of the Phosphorylated derivativeof FVSP (Ph-FVSP) were optimized based on single experiments and responsesurface analysis. Characterization and antioxidant properties of FVSP andPh-FVSP were investigated. The optimum phosphorylation modification process ofFVSP obtained was as follows: The mass ratio of STPP/STMP was 1:2, the reaction temperature was 70℃, the … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 3 · 16 Aug 2022 · 6,007 accessesView PDFCite
Research ArticleOpen accessIn the abstractPrevalence of Listeria monocytogenes in Raw Cow's Milk in the Region of Batna, AlgeriaSabrina Rabehi, Bakir Mamache, Taha Mossadak Hamdi et al.The aim of this study is the detection of the presence of L. monocytogenes in raw milk in the region of Batna using the real-time polymerase chain reaction (real-time PCR) technique, in order to assess the riskrun by the consumer of raw milk in the study area. 65 milk samples were investigated for the detection of L. monocytogenes. The detection … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 4 · 15 Oct 2021 · 6,997 accessesView PDFCite
Research ArticleOpen accessIn the abstractOptimized Biodiesel Production from jatropha curcas oilHaruna Musa, Daniel Eric, Alkhairu Hassan et al.In this study, a mathematical model for the trans-esterification reaction of jatropha curcas seed Oil (JCO) was developed using Response Surface Methodology(RSM) in terms of operational variables such as temperature (50 to 80°C), catalyst concentration (0.7 to 1% in relation to oil mass) and methanol/oil ratio (3:1-9:1), at fixed reaction time (90 min) and mixing intensity. Biodiesel was produced by … …Energy Research Journal, Vol. 12, Issue 1 · 12 Oct 2021 · 7,286 accessesView PDFCite
Research ArticleOpen accessIn the abstractAcetylation Process and Antioxidant Activity of a Polysaccharide from Loquat (Eriobotrya japonica) LeavesYiting Xue, Xiao Ding, Ze Zhang et al.Chemical modifications of polysaccharides can provide an opportunity to obtain new agents with possible therapeutic uses. In this study, a water-soluble polysaccharide extracted from Loquat Leaves (coded LLPs) was purified by the column chromatography, which was used to investigate its acetylation process by the acetic anhydride method. The Degree of Substitution (DS) was used as the index, the effect of … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 4 · 1 Jan 2021 · 7,056 accessesView PDFCite
Research ArticleOpen accessIn the abstractPrevalence of Diarrhea-Causing Pathogens in South Korea Analyzed Using Molecular Diagnostic MethodsYonghee Lee, Jae Kyung KimGastrointestinal inflammation remains a prevalent global health concern. Prompt and accurate diagnosis of the condition is required to mitigate health risks in immunocompromised individuals and alleviate the economic burden linked to treatment and pathogen isolation. Challenges posed by traditional culture tests, such as long detection time and inability to isolate low-abundance organisms, can be overcome using molecular diagnostic methods, which … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 2 · 25 Jul 2024 · 5,005 accessesView PDFCite
Research ArticleOpen accessIn the abstractAdipose-Derived Canine Mesenchymal Stem Cells in PLAY® MediaArjun Kulkarni, Bhavana N. V., Teena Mary Thomas et al.In veterinary medicine, stem cell-based therapies present promise for addressing orthopedic and degenerative conditions in canines. Mesenchymal Stem Cells (MSCs) possess unique regenerative, immunomodulatory, and differentiation capabilities. However, their efficacy relies on optimal in-vitro conditions. Prolonged expansion over passages leads to a gradual loss of stem cell traits, highlighting the importance of optimized culture conditions and media supplements. This study … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 3 · 13 Jul 2024 · 5,598 accessesView PDFCite