85 results for “Purification”

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Research ArticleOpen accessEvery word in the titlePurification, Structural Characterization and Anti-Aging and Stress Activities of Cordyceps Mycelium Polysaccharides from Paecilomyces HepialiXiaokang Hu, Huixiu Ma, Guicai Chen et al.This study identified and characterized the bioactive polysaccharide components from Cordyceps mycelium powder (CMP) derived from Paecilomyces hepiali, evaluating their anti-aging and stress-protective activities. Initial screening using Drosophila melanogaster revealed that crude Cordyceps polysaccharides (CCP) exhibited comparable effects to CMP and Cordyceps aqueous extracts (CAE) on natural lifespan and UV stress resistance, suggesting CCP as the primary bioactive component. CCP … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 4 · 16 Feb 2026 · 1,698 accessesView PDF Cite

Research ArticleOpen accessEvery word in the titleIntracellular L-Asparaginase from Bacillus sp. PG02: Purification, Biochemical Characterization and Evaluation of Optimum pH and TemperatureFatemeh Izadpanah Qeshmi, Mahsa Rahimzadeh, Sedigheh Javadpour, Manijeh PoodatBacterial L-asparaginases are amidohydrolases that act on L-asparagine and produce L-aspartate and ammonia. These enzymes have been used in treatment of lymphoblastic leukemia. In the present study, a novel strain, Bacillus sp. PG02 was explored for the production of intra-cellular L-asparaginase enzyme. The nitrogen source for L-asparaginase production was L-asparagine. New intracellular L-asparaginase was purified using ion exchange chromatography and … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 1 · 26 Nov 2015 · 7,480 accessesView PDF Cite

Research ArticleOpen accessEvery word in the titleBioactive Endophytic Actinomycetes of Cinnamomum sp.; Isolation, Identification, Activity Guided Purification and Process Optimization of Active MetaboliteSudipta Roy, Debdulal BanerjeeStudying on endophytic actinomycetes of Cinnamomum sp. from Indian rainforest, Cherapunji, one of our isolated strains designated as CH1 was found to produce significant antibacterial activity against test pathogens (Aeromonas caviae (ATCC 15468), Vibrio parahemolyticus ATCC 1782, Pseudomonas aeruginosa (ATCC 9027), Proteus vulgaris (ATCC 12453), Shigella flexnerii (ATCC 12022), Escherichia coli (human sample isolate), Bacillus subtilis (ATCC 11774) and Bacillus … …Current Research in Microbiology, Vol. 6, Issue 1 · 6 Apr 2015 · 9,062 accessesView PDF Cite

Research ArticleOpen accessEvery word in the titlePurification of Natural Gas with Impurities using Membrane Processes: Parameter EstimationN. W. Jusoh, K. K. Lau, A. M. ShariffProblem statement: Membrane processes have received a significantly high attention on natural gas separation processes since this technology provides higher operating and cost advantages as compared to other technologies. One of the major problems related to natural gas separation using membrane is the existence of feed impurities including CO2, heavy hydrocarbon and water. Approach: Hence, mathematical model that is able … …American Journal of Engineering and Applied Sciences, Vol. 5, Issue 1 · 22 May 2012 · 8,426 accessesView PDF Cite

Research ArticleOpen accessEvery word in the titleA Putative Serine protease from Larval Midgut of Red Palm Weevil Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae): Partial Purification and Biochemical CharacterizationAhmed Al Jabr, Mahmoud Abo-El-SaadA putative serine protease was isolated and partially purified from larval midgut of red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier) using anion exchange chromatography, DE-52. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of this protease showed that the major band has a molecular weight of approximately 24 kDa corresponding to the same molecular weight of mammalian trypsin. In addition, zymography … …American Journal of Environmental Sciences, Vol. 4, Issue 6 · 31 Dec 2008 · 7,018 accessesView PDF Cite

Research ArticleOpen accessEvery word in the titleUse of Barley for the Purification of Aquaculture Wastewater in a Hydroponics SystemA.M. Snow, A.E. GhalyBarley was examined for its ability to remove nutrients from aquaculture wastewater. The effects of seed sterilization using ethanol and bleach and seed density on germination and plant growth were investigated. Surface sterilization of barley seeds had a negative impact on germination. Increasing the ethanol concentration and/or the bleach concentration reduced the germination percentage. Barley seeds were first germinated in … …American Journal of Environmental Sciences, Vol. 4, Issue 2 · 30 Apr 2008 · 7,484 accessesView PDF Cite

Research ArticleOpen accessEvery word in the titleA Comparative Study of the Purification of Aquaculture Wastewater Using Water Hyacinth, Water Lettuce And Parrot’s FeatherA. M. Snow, A. E. GhalyWater hyacinth, water lettuce and parrot’s feather plants were examined for their ability to remove nutrients from aquaculture wastewater at two retention times. During the experiment, the aquatic plants grew rapidly and appeared healthy with green color. At hydraulic retention times (HRTs) of 6 and 12 days, the average water hyacinth, water lettuce and parrot’s feather yields were 83, 51 … …American Journal of Applied Sciences, Vol. 5, Issue 4 · 30 Apr 2008 · 7,858 accessesView PDF Cite

Research ArticleOpen accessEvery word in the titleA Comparative Assessment of Hydroponically Grown Cereal Crops for the Purification of Aquaculture Wastewater and the Production of Fish FeedA. M. Snow, A. E. Ghaly, A. SnowHydroponically grown wheat, barley and oats were examined for their ability to remove nutrients from aquaculture wastewater. Wheat, barley and oats seeds were germinated in water in a hydroponics system. The seedlings then received wastewater from an aquaculture system stocked with Arctic charr. During the experiment, the crops grew rapidly and fairly uniformly and showed no signs of mineral deficiency … …American Journal of Agricultural and Biological Sciences, Vol. 3, Issue 1 · 31 Mar 2008 · 9,167 accessesView PDF Cite

Research ArticleOpen accessEvery word in the titleIsolation and Purification of Bacterial Strains from Treatment Plants for Effective and Efficient Bioconversion of Domestic Wastewater SludgeK. C.A. Jalal, Md. Z. Alam, Suleyman A. Muyibi, P. JamalForty six bacterial strains were isolated from nine different sources in four treatment plants namely Indah Water Konsortium (IWK) sewage treatment plant, International Islamic University Malaysia (IIUM) treatment plant-1,-2 and -3 to evaluate the bioconversion process in terms of efficient biodegradation and bioseparation. The bacterial strains isolated were found to be 52.2% (24 isolates) and 47.8% (22 isolates) in the … …American Journal of Environmental Sciences, Vol. 2, Issue 1 · 31 Mar 2006 · 7,575 accessesView PDF Cite

Research ArticleOpen accessEvery word in the titleExpression, Purification and Activity Assay of Two New Recombinant Antagonists of Fibrinogen ReceptorJianbo Yang, Jia Yao, Kun Yang et al.The gene sequence of Decorsin which is extracted from a kind of North American leeches was synthesized. Two recombinant proteins, Annexin V plus Decorsin (AnnV-D39) and Annexin V plus the carboxyl terminal 27 amino acid residues of Decorsin(AnnV-D27), were constructed. And a 10 amino acids linker peptide of GGGGSGGGGS was inserted between Annexin V and Decorsin in AnnV-D39. Using pET-28(a+) … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 2 · 30 Jun 2005 · 6,913 accessesView PDF Cite