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20 articles for “Protease Activity”
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Research ArticleOpen accessIn the abstractEffects of Cement Dust on the Physiological Activities of Arabidopsis thalianaSaeid Abu-Romman, Jarrah AlzubiAir pollution exerts detrimental effects on plant ecosystems and restricts agricultural productivity. Cement dust is considered to be one of the most harmful air pollutants of industrial origin and is a limiting factor for plant growth and productivity. This study aims to uncover the impacts of cement dust on photosynthetic pigments, protein content, hydrogen peroxide (H2O2) accumulation, lipid per oxidation … …American Journal of Agricultural and Biological Sciences, Vol. 10, Issue 4 · 17 Nov 2015 · 8,113 accessesView PDFCite
Research ArticleOpen accessIn the abstractHide Dehairing and Laundry Detergent Compatibility Testing of Thermostable and Solvents Tolerant Alkaline Protease from Hot Spring Isolate Bacillus cohniiU3Pravin R. Dudhgara, Bhavsar Sunil, Ghelani AnjanaIn the present study, alkaline protease producing thermophilic Bacillus cohniiU3 strain was isolation from Unnai hot spring, India. The maximum production of protease (344 U/mL) was reported after 72 h of inoculation at 50°C in shake flask culture using the gelatin casein medium. The protease was found to remain active up to 96 h and production was growth dependent. The … …OnLine Journal of Biological Sciences, Vol. 15, Issue 3 · 23 Jul 2015 · 7,335 accessesView PDFCite
Research ArticleOpen accessIn the abstractCorrelation Between in vitro Biofilm Formation and Virulence Properties of Extra-Intestinal Pathogenic Escherichia Coli (Expec)Md. Fakruddin, Khanjada Shahnewaj Bin Mannan, Reaz Mohammad MazumdarAbout 65 Escherichia coli strains isolated from extra-intestinal sites were included in this study to determine their biofilm formation capability and virulence properties. About 87% ExPEC isolates were motile and 12.3% were found to be non-motile. Only 12.30% isolates were strong biofilm former (SBF >1.5) whereas 18.46% isolates were non biofilm former. About 71% isolates were capable to express curli … …OnLine Journal of Biological Sciences, Vol. 14, Issue 4 · 14 Jan 2015 · 6,942 accessesView PDFCite
Research ArticleOpen accessIn the abstractIdentification of Novel Human Immunodeficiency Virus-1 Integrase Inhibitors by Shape-Based Virtual ScreeningT.K. Omprakash, A. Thamarai Selvan, A. Shahul Hameed, S.P. GeethaProblem statement: Human Immunodeficiency Virus-1 (HIV-1) is causative agent of the immune system disease, Acquired Immune Deficiency Syndrome (AIDS). Majority of anti-HIV drugs target reverse transcriptase and protease enzymes. Toxicity and development of multidrug resistant HIV-1 virus strains are the reasons for studying new targets in HIV-1 replication process for identifying novel inhibitor with low toxicity and high activity. Approach: … …Current Research in Medicine, Vol. 1, Issue 2 · 31 Dec 2010 · 7,191 accessesView PDFCite
Research ArticleOpen accessIn the abstractA Proteomics Analysis of Drought Stress-Responsive Proteins as Biomarker for Drought-Tolerant Sugarcane CultivarsNisachon Jangpromma, Supansa Kitthaisong, Khomsorn Lomthaisong et al.Problem statement: The prime objective in breeding selection process of drought-tolerant sugarcane is to identify the correlating marker, which could lead to rapid screening for drought-tolerant cultivars. In this study, we have reported an unknown 18-kDa protein (p18) along with other stress-inducible proteins to be highly expressed in sugarcane leaves under drought stress condition. Approach: The 2D-PAGE patterns of proteins … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 2 · 30 Jun 2010 · 9,814 accessesView PDFCite
Research ArticleOpen accessIn the abstractA Putative Serine protease from Larval Midgut of Red Palm Weevil Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae): Partial Purification and Biochemical CharacterizationAhmed Al Jabr, Mahmoud Abo-El-SaadA putative serine protease was isolated and partially purified from larval midgut of red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier) using anion exchange chromatography, DE-52. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of this protease showed that the major band has a molecular weight of approximately 24 kDa corresponding to the same molecular weight of mammalian trypsin. In addition, zymography … …American Journal of Environmental Sciences, Vol. 4, Issue 6 · 31 Dec 2008 · 7,018 accessesView PDFCite
Research ArticleOpen accessIn the abstractProtective Effects of Covalent Cross-Linking on Proteolysis of Human Coproporphyrinogen Oxidase and Implications for PorphyriaAhmed W. Jafri, Jason R. Stephenson, Justin B. Morgenthaler et al.The effects of covalent cross-linkers on the enzyme, coproporphyrinogen oxidase, had been previously studied but their role in protecting the enzyme from protease cleavage has not been evaluated. Therefore, we examined how the cross-linker bis (sulfosuccinimidyl) suberate (BS3) affects the ability of trypsin to digest purified, wild type recombinant human coproporphyrinogen oxidase and selected mutants. Following incubation, the apparent molecular … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 4 · 31 Dec 2008 · 6,687 accessesView PDFCite
Research ArticleOpen accessIn the abstractCharacterization and Wash Performance Analysis of Microbial Extracellular Enzymes from East Calcutta Wetland in IndiaRamesh Malathu, Sanhita Chowdhury, Madhusmita Mishra et al.Extracellular protease from a novel bacterial isolate showing maximum similarity of 98.22% with Microbacterium luteolum was obtained from East Calcutta Wetland, India. It showed compatibility with commercial detergents. The enzyme retains more than 60% of its activity between 6.0 to 10.5 pH. The maximum activity is at pH 7.5 with 71% activity at pH 10.0 and 10.5. The protease retained … …American Journal of Applied Sciences, Vol. 5, Issue 12 · 31 Dec 2008 · 8,727 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Proteolytic System of Candida dubliniensisLoaiza-Loeza Salomé, Parra-Ortega Berenice, Bautista-Muñoz Consuelo et al.Proteases of Candida dubliniensis have been scarcely studied, these enzymes may play an important role in nitrogen metabolism, post-translational processing, nutritional stress, dimorphism, virulence, etc. In this work, we report the presence of five different intracellular proteases and one extracellular proteolytic activity. The intracellular proteases are: aminopeptidase ycdAPE, carboxypeptidase ycdCP, dipeptidyl aminopeptidase ycdDAP, proteinases ycdPrA and ycdPrB, and extracellular protease … …American Journal of Infectious Diseases, Vol. 3, Issue 2 · 30 Jun 2007 · 6,953 accessesView PDFCite
Research ArticleOpen accessIn the abstractHIV-1-Specific CTL Recognizing a Scarce Epitope Are Not Suppressed by HIV-1 Protease InhibitorsOtto O. YangHIV-1-protease inhibitors have received growing attention as agents that can affect proteases in human proteasomes, with potentially deleterious effects for HIV-1-specific cellular immunity. Studying the antiviral activity of HIV-1-specific CTL that recognize an epitope that is presented at limiting concentrations on infected cells, we find that several HIV-1 protease inhibitors do not affect their function. These data indicate that these … …American Journal of Immunology, Vol. 3, Issue 1 · 30 Jun 2007 · 7,016 accessesView PDFCite