15 results for “Polyacrylamide”

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Research ArticleOpen accessIn the abstractStudy and Identification of Insulin-Like Growth Factor-I Gene Polymorphisms in Zel Sheep PopulationSeyed Mohsen Kazemi, Cyrus Amirinia, Hossein Emrani, Shahaboddin GharahveysiProblem statement: The IGFs play an important role in regulating somatic growth according to nutritional conditions. Polymorphisms of IGF gene are reported to be significantly associated with many traits. Approach: In these study 142 samples of DNA from Zel sheep were used for detecting the polymorphisms of promoter region of the Insulin-like Growth Factor-1 (IGF-1) gene. Therefore DNA extracted from … …American Journal of Animal and Veterinary Sciences, Vol. 6, Issue 4 · 13 Jan 2012 · 7,739 accessesView PDF Cite

Research ArticleOpen accessIn the abstractA Putative Serine protease from Larval Midgut of Red Palm Weevil Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae): Partial Purification and Biochemical CharacterizationAhmed Al Jabr, Mahmoud Abo-El-SaadA putative serine protease was isolated and partially purified from larval midgut of red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier) using anion exchange chromatography, DE-52. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of this protease showed that the major band has a molecular weight of approximately 24 kDa corresponding to the same molecular weight of mammalian trypsin. In addition, zymography … …American Journal of Environmental Sciences, Vol. 4, Issue 6 · 31 Dec 2008 · 7,018 accessesView PDF Cite

Research ArticleOpen accessIn the abstractPlatelet Derived Growth Factor-A mRNA Levels in Diabetic and Nondiabetic Subjects at Risk of Coronary Heart DiseaseSalman A.H. AlrokayanCoronary heart disease (CHD) is the number one cause of death in developed countries. We investigated the quantity of platelet derived growth factor-A mRNA (PDGF-A mRNA) among four different groups of diabetic and nondiabetic Saudi subjects (n = 40) at risk of CHD. Total RNA was derived from peripheral blood mononuclear cells (PBMN) and PDGF-A mRNA reverse transcribed into cDNA … …American Journal of Biochemistry and Biotechnology, Vol. 3, Issue 3 · 30 Sep 2007 · 6,676 accessesView PDF Cite

Research ArticleOpen accessIn the abstractRemoval and Recovery of Uranium using Microorganisms Isolated from North American Uranium DepositsTakehiko TsurutaSome attempts were made to remove and recover uranium that may be present in nuclear fuel effluents and mine tailings using microorganisms isolated from North American uranium deposits. To establish which microorganisms accumulate the most uranium, hundreds strains of microorganisms were screened. Of these strains of microorganisms tested, extremely high uranium accumulating ability was found in some bacteria isolated from … …American Journal of Environmental Sciences, Vol. 3, Issue 2 · 30 Jun 2007 · 7,285 accessesView PDF Cite

Research ArticleOpen accessIn the abstractA Recursive Application of a Support Vector Machine for Protein Spot Detection in 2-Dimensional Gel ElectrophoresisGary D. Boetticher, Hisham Al-Mubaid, Karen Frasier-ScottTwo-dimensional polyacrylamide gel electrophoresis (2-D PAGE) analysis remains the core of proteomic technology because it is currently the most powerful method to analyze large collections of proteins. Advances in electrophoresis equipment are making this technique more accessible but effective computer assisted protein spot detection remains a very labor-intensive endeavor. Protein spot analysis is still time consuming, requires human intervention and … …Journal of Computer Science, Vol. 1, Issue 3 · 30 Sep 2005 · 6,675 accessesView PDF Cite