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21 articles for “Nucleotides”
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Research ArticleOpen accessIn the abstractA NEW PRESUMED IGKAPPA GENE IMPLIED IN THE SEA STAR IMMUNE RESPONSEMichel Leclerc, Magne Osteras, Patricia OttenThe existence of another candidate IgKappa gene of 697 nucleotides, was recently, demonstrated, in the sea star Asterias rubens genome. This last gene was cloned and shown, with its sequence, in the present study. Its potential role in the sea star immune response was accentuated. …American Journal of Immunology, Vol. 10, Issue 1 · 19 Mar 2014 · 6,788 accessesView PDFCite
Research ArticleOpen accessIn the abstractPHYLOGENETIC ANALYSIS OF THE SWINE LEUKOCYTE ANTIGEN-6 GENE GENERATED FROM SPLEENTISSUE OF KOREAN NATIVE PIGJaeyoung Kim, Hyojun Park, Yoonchung Choi, Hoyoung ChungThis study was aimed to search genetic variants, to investigate phylogenetic relationships between pig breeds and to provide basic genetic information of Korean Native Pigs (KNP) using the variations of the Swine Leukocyte Antigen-6 (SLA-6) gene. Cloning of the Swine Leukocyte Antigen (SLA)-6 gene in the MHC non-classical region was performed with spleen tissues of Korean Native Pigs (KNP). Sequencing … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 1 · 22 Feb 2014 · 7,234 accessesView PDFCite
Research ArticleOpen accessIn the abstractRANDOMLY AMPLIFIED POLYMORPHIC DNA-A BRIEF REVIEWNandani Kumari, Saroj Kumar ThakurRAPD is a PCR based technique which involves the use of single arbitrary short primers (8-12 nucleotides), resulting in the amplification of many discrete DNA. The segments of DNA that are amplified are random. The technique was developed independently by two different laboratories and called as RAPD and AP-PCR (Arbitrary Primed PCR). This procedure detects nucleotide sequence polymorphisms in a … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 1 · 2 Jan 2014 · 17,258 accessesView PDFCite
Research ArticleOpen accessIn the abstractANOTHER CANDIDATE IG KAPPA-LIKE GENE IN THE SEA STAR A. RUBENSMichel LeclercIn a recent paper we have shown that Ig Kappa genes (region MOPC 63, region K2), of 250 nucleotides each, were present in the sea star Asterias rubens. Two years later, a 697 nucleotide Ig Kappa chain V-V region K2 gene was accentuated. …American Journal of Immunology, Vol. 10, Issue 1 · 20 Dec 2013 · 6,758 accessesView PDFCite
Research ArticleOpen accessIn the abstractDetecting Patterns in 5’ Untranslated Regions of Genes Involved in the Regulation of Blood Pressure and Oxidative StressMaria Sanchez-Aguilar, Luz Ibarra-Lara, Beatriz Zamora-Lopez et al.Hypertension is a chronic physical condition with multifactorial causes. Although genetic factors have been associated to this pathological condition, characterization of the sequence patterns in the regulatory sites, for example, in 3’ or 5’ Untranslated Regions (UTR), remains to be explored. In particular, the 5’ UTR of genes associated with the regulation of blood pressure and oxidative stress are analyzed … …Current Research in Medicine, Vol. 4, Issue 2 · 27 Nov 2013 · 7,283 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Bacterial Chromosomal Sequence and Related IssuesThi Huyen Phan, Duc Luong NguyenThere has been a rapid increasing in the number of bacterial genomes that are completely sequenced recently. However, nucleotide arrangement in bacterial chromosome is still not clear, though it is known that the chromosomal strand is symmetric as a whole but asymmetric locally. The unequal distribution of adenine and thymine as well as cytosine and guanine in the local genomic … …Current Research in Virology, Vol. 2, Issue 1 · 29 Jul 2013 · 13,208 accessesView PDFCite
Research ArticleOpen accessIn the abstractComparative Physiological and Molecular Study of Some Sheep Breeds in Saudi ArabiaMohammad S. AL-Harbi, Hamed S. Alwagdany, Sayed A.M. AmerNaemi, Heri, Najdi and Sawakni sheep breeds inhabiting Kingdom of Saudi Arabia were studied on both physiological and molecular bases. Native polyacrylamide gel-electrophoreses for two enzymes Malate Dehydrogenase (Mdh) and Malic Enzyme (Me) recorded 5 isoenzyme loci from which 4 were polymorphic and 1 was monomorphic. Mdh showed three fractions, the first was recorded in Sawakni and Najdi breeds, the … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 2 · 20 Jun 2013 · 8,388 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Study of Energy Related Mitochondrial Genes in Arabian and Bactrian CamelsMohamed M. Ahmed, Samir A. El-Shazly, Samy M. Sayed, Sayed A.M. AmerThe single-humped camel, Camelus dromedaries inhabiting Afro-Arabia and the double-humped camel, Camelus bactrianus inhabiting central Asia are the only species in their genus. The present study aimed to amplify and partially sequence the mitochondrial DNA genes encoding for NADH dehydrogenase subunit 1, cytochrome c oxidase subunit 1, ATP synthase subunit 6 (ATP6), cytochrome b and displacement region (d-loop) in the … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 1 · 18 Mar 2013 · 7,368 accessesView PDFCite
Research ArticleOpen accessIn the abstractPyrosequencing-An Alternative to Traditional Sanger SequencingMd Fakruddin, Abhijit ChowdhuryProblem statement: Pyrosequencing has the potential to rapidly and reliably sequence DNA taking advantages over traditional Sanger di-deoxy sequencing approach. Approach: A comprehensive review of the literature on the principles, applications, challenges and prospects of pyrosequencing was performed. Results: Pyrosequencing was a DNA sequencing technology based on the sequencing-by-synthesis principle. It employs a series of four enzymes to accurately detect … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 1 · 2 Mar 2012 · 12,887 accessesView PDFCite
Research ArticleOpen accessIn the abstractNucleotide Sequence of the Coat Protein Gene of the Malaysian Passiflora Virus and its 3' Non-Coding RegionNorzihan Abdullah, Ismanizan Ismail, Vilasini Pillai et al.Problem statement: In this study, we identified the full length Coat Protein (CP) gene of the Malaysian Passiflora Virus (MPV) and its 3' non-coding region. The CP gene of the MPV contained 285 amino acid residues. Approach: Pairwise comparison of the MPV CP region with four other potyviruses, namely East Asian Passiflora Virus (EAPV), Passionfruit Woodiness Virus (PWV), Bean Common … …American Journal of Applied Sciences, Vol. 6, Issue 9 · 30 Sep 2009 · 7,723 accessesView PDFCite