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74 articles for “Molecular Characterization”

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Research ArticleOpen accessPhrase in the titleMOLECULAR CHARACTERIZATION OF SARCOCYTIS FUSIFORMIS AND SARCOCYSTIS BUFFALONIS INFECTING WATER BUFFALOES (BUBALUS BUBALIS) FROM EGYPTMahmoud El-Seify, Ahmed El-Morsey, Mosaad Hilali et al.Parasites of the genus Sarcocystis have the ability to infect wide range of animals, birds and reptiles. Water buffaloes are intermediate hosts for four Sarcocystis species; S. fusiformis, S. buffalonis, S. levinei and S. dubeyi. There was no previous molecular identification of Sarcocystis spp. parasitizing water buffaloes from Egypt. The current study was established to screen infection rates and apply … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 2 · 16 Apr 2014 · 7,938 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleThe Porcine Mitochondrial Transcription Factor a Gene: Molecular Characterization, Radiation Hybrid Mapping and Genetic Diversity among 12 Pig BreedsTanja Kunej, Xiao-Lin Wu, Jennifer J. Michal et al.Problem statement: Mitochondrial transcription factor A (TFAM) is a nucleus-encoded protein that is a key activator of mitochondrial transcription as well as a major participant in mitochondrial genome replication. Genomic characterization of the porcine TFAM gene is, therefore, necessary to determine its involvement in regulation of fat depots and meat quality traits in pigs. Approach: Genomic DNA sequence was determined … …American Journal of Animal and Veterinary Sciences, Vol. 4, Issue 4 · 31 Dec 2009 · 6,870 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleMolecular Characterization of Pseudomonas aeruginosa UPM P3 from Oil Palm RhizosphereBadri Fariman Azadeh, Sariah MeonProblem statement: Pseudomonas aeruginosa has been used in agriculture as biological agents. It has shown substantial control of a variety of soil-borne plant pathogens including Macrophomina phaseolina, Botrytis cinerea, Rhizoctonia solani, Colletotrichum truncatum, Pythium, Fusarium and others. Species aggregate of Pseudomonas aeruginosa strain UPM P3 was shown to have potential as a biocontrol agent against Ganoderma boninense, the causal agent … …American Journal of Applied Sciences, Vol. 6, Issue 11 · 30 Nov 2009 · 7,802 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleMolecular Characterization of a Phytoplasma Associated with Coconut Yellow Decline (CYD) in MalaysiaN. Nejat, K. Sijam, S. N.A. Abdullah et al.Problem statement: Molecular methods have been used to detect phytoplasma in Malayan Red Dwarf (MRD) ecotype of coconut for the first time in Malaysia. Detect phytoplasma from coconut showing yellowing symptoms by nested PCR and Classify phytoplasma associated with disease of coconut palms, based on analysis of 16S rRNA gene operon sequences and virtual RFLP. Approach: Twenty MRD symptomatic palms … …American Journal of Applied Sciences, Vol. 6, Issue 7 · 31 Jul 2009 · 7,919 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleMolecular Characterization of Opium Poppy (Papaver somniferum) GermplasmHari Shanker Acharya, Vimal SharmaProblem statement: The medicinal value of opium poppy (2n = 22, Papaver somniferum L.) is due to presence of more than two dozen alkaloids. The genetic origin and diversity of cultivars used in the breeding collection is not fully known. To evaluate the genetic diversity of the 24 germplasm currently cultivated in the state to provide genetic information about heterogeneity, … …American Journal of Infectious Diseases, Vol. 5, Issue 2 · 30 Jun 2009 · 8,096 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleMolecular Characterization of Soybean Mosaic Virus NIa Protein and its Processing Event in Bacterial ExpressionBong K. Choi, Jae S. Park, Hye J. Ahn et al.Soybean mosaic virus (SMV)-CN18 is an Rsv resistance-breaking (RB) isolate to overcome soybean resistance genes Rsv1, Rsv3 and Rsv4. The aim of this study was to characterize nuclear inclusion protein a (NIa protein) of RB isolate at the molecular level and demonstrate its processing into genome-linked protein (VPg) and NIa-Pro domains in Esherichia coli containing a bacterial expression pET vector … …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 4 · 31 Dec 2006 · 6,902 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleMolecular Characterization of Banana (AA) Diploids with Contrasting Levels of Black and Yellow Sigatoka ResistanceClaudia Fortes Ferreira, Sebastiao Oliveira Silva, Nivia Paula Damasceno Sobrinho et al.Most banana cultivars are susceptible to many diseases, whereas Sigatoka leads to greatest yield losses. One of the strategies to overcome this disease is thorough banana genetic breeding which consists in the obtainment of improved (AA) diploids which are then crossed with triploids obtaining (AAAB) tetraploid disease resistant bananas also presenting other important agronomic characteristics. The prior knowledge of the … …American Journal of Applied Sciences, Vol. 1, Issue 4 · 31 Dec 2004 · 7,652 accessesView PDFCite
Research ArticleOpen accessPhrase in the titlePrevalence of Escherichia Coli in Some Selected Foods and Children Stools with Special Reference to Molecular Characterization of Enterohemorrhagic StrainR. Farhan, S. Abdalla, H. A. Abdelrahaman et al.Escherichia coli is one of the most important etiologic agents of childhood diarrhea that represents a major public health problem in developing countries and now is being recognized as emerging entero-pathogens in the well developed countries. Among the diarrhegenic Escherichia Coli (DEC) this work was focused on the Enterohemorrhagic Escherichia Coli (EHEC) that produce Shigatoxins (Stxs). The infection is mainly … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 4 · 23 Dec 2014 · 7,873 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleMOLECULAR CHARACTERIZATION ON THE BASIS OF HA, NA AND M GENE REVEALED CHANGES IN CRITICAL AMINO ACID POSITIONS OF INFLUENZA A (H3N2) VIRUS CIRCULATING IN INDIA DURING 2011-2013Sachin Kumar, Shashi Khare, Bano Saidullah et al.Influenza a (H1N1) virus is responsible for acute respiratory infection in human, which occasionally causes epidemic and rarely a pandemic. Full length DNA sequencing of HA, NA and M gene of H3N2 virus from year 2011-2013 was performed for determination of the circulating strains in India along with monitoring status of various mutations associated with drug resistance and virulence. Genetic … …American Journal of Infectious Diseases, Vol. 10, Issue 3 · 18 Oct 2014 · 6,839 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleMolecular Characterization of Assam Hill GoatGalib Uz Zaman, Naba Nahardeka, Subimal Laskar et al.A total of 23 polymorphic microsatellite markers were used to evaluate genetic diversity and population structure in Assam Hill Goat (AHG). All the loci studied were polymorphic in nature. The number of observed alleles (Na) detected ranged from 2 to 10 with an overall mean of 4.9±2.220. A total of 114 alleles were observed across all the loci. The effective … …American Journal of Animal and Veterinary Sciences, Vol. 8, Issue 2 · 13 Jul 2013 · 7,458 accessesView PDFCite