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28 articles for “High Performance Liquid Chromatography (HPLC)”
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Research ArticleOpen accessIn the abstractEffect of Pre-Slaughter Autophagic Status on Postmortem Proteolysis in Skeletal Muscle of MiceTomonori Nakanishi, Miho Yamashita, Naoto Nishimoto et al.Post mortem proteolysis is a key event during the aging process, where muscle converts to meat. However, its precise mechanism remains unclear. In this study, the effects of pre-slaughter autophagic status on post mortem proteolysis were investigated using mouse skeletal muscle. Mice were fasted for 14 h and administered with or without the autophagy inhibitor wortmannin, whereas control mice were … …American Journal of Animal and Veterinary Sciences, Vol. 12, Issue 2 · 10 Jul 2017 · 7,988 accessesView PDFCite
Research ArticleOpen accessIn the abstractDetermination of Abscisic Acid and its Relationship to Drought Stress Based on Cowpea Varieties with Different Capability of Drought ResistanceWei Lei, Shian Huang, Shaohu Tang et al.Cowpea (Vigna unguiculata L.) is one of the most important grain legume supplying necessarily dietary proteins. Although its can tolerate drought environment, still the severe condition can limit the growth and development of this plant. Abscisic acid (ABA) has a crucial function in stress physiology, but little information is available for methods describing ABA determination in cowpea and its relationship … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 1 · 30 Mar 2016 · 8,458 accessesView PDFCite
Research ArticleOpen accessIn the abstractAnti-Inflammatory, Analgesic and Antioxidant Activities of Allophylus Cobbe LeavesPreeti Jain, Md. Selim Hossain, Kaniz Fatema et al.Allophylus cobbe L. Raeuschel (Family-Sapindaceae) is a medicinal plant used traditionally for the treatment of various health risks like pain, inflammation, ulcers and wounds in Bangladesh. This study determined the polyphenolic compounds and evaluated the analgesic, anti-inflammatory and antioxidant effects of the ethanol extract of Allophylus cobbe leaves. High Performance Liquid Chromatography (HPLC) analysis was used to determine the polyphenolic … …American Journal of Pharmacology and Toxicology, Vol. 9, Issue 4 · 27 Jan 2015 · 8,152 accessesView PDFCite
Research ArticleOpen accessIn the abstractCOLORIMETRIC DETERMINATION OF CEFQUINOME SULPHATE IN BULK AND DOSAGE FORM USING AMMONIUM MOLYBDATEShaza Wageialla Shantier, Elrasheed Ahmed GadkariemThis study was designed to develop two colorimetric methods for the determination of Cefquinome Sulphate (CS) in bulk and dosage forms using two different concentrations of molybdenum solution. The developed methods were based on the oxidation of CS with 2% ammonium molybdate, in the presence of sulphuric acid, producing a green colored product with λmax at 409 nm (Method I) … …American Journal of Applied Sciences, Vol. 11, Issue 2 · 20 Dec 2013 · 7,051 accessesView PDFCite
Research ArticleOpen accessIn the abstractINVESTIGATION OF STABILITY OF KOREAN GINSENG IN HERBAL DRUG PRODUCTLatifa Bashir Babiker, Elrasheed Ahmed Gadkariem, Riyadh Mohammed Alashban, Haya Ibrahim ALjoharThis study was designed to investigate long term stability of K-Gin capsules, a herbal medication that contains korean ginseng. Stability studies ensuring the maintenance of product quality, safety and efficacy throughout the shelf life are considered as pre-requisite for the acceptance and approval of any pharmaceutical product. Conducting long term stability studies following the guidelines issued by ICH, WHO and … …American Journal of Applied Sciences, Vol. 11, Issue 1 · 18 Dec 2013 · 9,221 accessesView PDFCite
Research ArticleOpen accessIn the abstractBioautography Guided Identification of Anticandidal Compounds from A. terreus st. 1B. K. Nagaraja, R. Sripathy, C. N. Ramchand, M. SundararamanProblem statement: Saprophytic and soil inhabiting microorganisms are prolific producers of secondary metabolites with wide range of structural and functional diversity. The objective of the current study was to screen and evaluate the anti-candidal properties of soil inhabiting fungi. Approach: Preliminary dual culture assay and bioautography was used to screen the fungi with potential anti-fungal activity and direct detection of … …American Journal of Infectious Diseases, Vol. 7, Issue 4 · 1 Dec 2011 · 7,629 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Comparison of Spectrophotometric Method and High-Performance Liquid Chromatography in Photosynthetic Pigments AnalysisSabina Jodłowska, Adam LatałaProblem statement: Spectrophotometric method and High-Performance Liquid Chromatography (HPLC) are the most useful tools to photosynthetic pigments analysis. The main objective of this reaserch was to compere the results obtained by these two independent methods in experiments under controlled laboratory conditions. Approach: Comparison between spectrophotometric and HPLC methods for pigment analysis was carried out on Baltic cyanobacterium Geitlerinema amphibium, which … …OnLine Journal of Biological Sciences, Vol. 11, Issue 2 · 30 Sep 2011 · 10,865 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Plant Growth Regulators on Callus, Cell Suspension and Cell Line Selection for Flavonoid Production from Pegaga (centella asiatica L. urban)Suat Hian Tan, Radzali Musa, Arbakariya Ariff, Mahmood MaziahProblem statement: Considering pegaga medicinal properties and over-exploitation, the requirement for a tissue culture technique as an alternative production system was crucial. Approach: Investigation of cell suspension culture response to different plant growth regulators (PRGs) for flavonoid production from elite cell line was carried out. Callus cultures were initiated from the leaf explants of Centella asiatica on Murashige and Skoog … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 11,254 accessesView PDFCite
Research ArticleOpen accessIn the abstractValidated High Performance Liquid Chromatographic Method for Analysis of Fenvalerate Pesticide in Chilies by QuEChERS Extraction Cleanup and High Liquid ChromatographyAhmed Ambarak Altorshani, Nor Kartini Abu Bakar, Eltayeb Elamin M. EidProblem statement: Contamination associated with pesticide use has increased as well, adversely impacting the environment and causing human health risks through residues on food. Approach: This study reports the extraction of Fenvalerate (FE) in chilies using QuEChERS based on High Performance Liquid Chromatography (HPLC) describes a simple, fast and inexpensive method. The chillie was extracted with acetonitrile Aliquots were cleaned-up … …American Journal of Applied Sciences, Vol. 7, Issue 12 · 31 Dec 2010 · 7,163 accessesView PDFCite
Research ArticleOpen accessIn the abstractCharacterization of Epoxidized and Non-Epoxidized Fatty Diethanolamides by High Performance Liquid Chromatography and Gas ChromatographyChoy Sin Lee, Song Cang LeeProblem statement: This study was carried out for the characterization of epoxidized and non-epoxidized fatty diethanolamides by High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC). Approach: The fatty diethanolamides were synthesized by reacting 40% (w/w) of Epoxidized Palm Olein (EPOo) with 60% (w/w) Refined Bleached Deodorized Plam Kernel Olein (RBDPKOo) with Diethanolamine (DEA) at 110°C for 5 h. Results: … …American Journal of Pharmacology and Toxicology, Vol. 5, Issue 3 · 30 Sep 2010 · 7,449 accessesView PDFCite