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27 articles for “Enzyme Properties”
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Research ArticleOpen accessIn the abstractBiochemical Indicators and Nutritional Value of Horsemeat Infected by Parascaridosis and Sampled from the Surrounding Areas of Semipalatinsk Nuclear Test Site, KazakhstanShyngys Suleimenov, Sergazy Duyssembaev, Ainur Serikova et al.Due to high prevalence of the Parascaris equorum among horses, the effect of Paracardiosis infestation on nutritional and biological values of meat is required. In an effort to analyze the radionuclides contents, biochemical parameters and biological value of horsemeat collected from different areas of Semipalatinsk Nuclear Test Site (SNTS) of Kazakhstan. The prevalence of parascaridosis was performed following standard protocols. … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 1 · 9 Feb 2021 · 6,606 accessesView PDFCite
Research ArticleOpen accessIn the abstractIntracellular L-Asparaginase from Bacillus sp. PG02: Purification, Biochemical Characterization and Evaluation of Optimum pH and TemperatureFatemeh Izadpanah Qeshmi, Mahsa Rahimzadeh, Sedigheh Javadpour, Manijeh PoodatBacterial L-asparaginases are amidohydrolases that act on L-asparagine and produce L-aspartate and ammonia. These enzymes have been used in treatment of lymphoblastic leukemia. In the present study, a novel strain, Bacillus sp. PG02 was explored for the production of intra-cellular L-asparaginase enzyme. The nitrogen source for L-asparaginase production was L-asparagine. New intracellular L-asparaginase was purified using ion exchange chromatography and … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 1 · 26 Nov 2015 · 7,484 accessesView PDFCite
Research ArticleOpen accessIn the abstractProbing the Substrate Specificity and Intersubunit Interactions of Brevundimonas Diminuta Glutaryl Acylase with Site-Directed MutagenesisMichael Eldarov, Anna Lyashenko, Tatyana Sherbakova et al.Glutaryl-7-ACA acylases (GLA) are industrial enzymes widely used for the production of 7-aminocepahlosporanic acid (7-ACA)-the starting compound for manufacturing of semisynthetic cephalosporin antibiotics. Generation of mutant GLA's with increased activity and stability, capability for single-step conversion of cephalosporin C (CPC) directly to 7-ACA is a promising route to improve the current biocatalytic technologies. In this study GLA from Brevundimonas diminuta … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 3 · 12 Nov 2014 · 8,164 accessesView PDFCite
Research ArticleOpen accessIn the abstractLIPASE-CATALYZED TRANSESTERIFICATION OF PALM KERNEL OIL WITH DIALKYLCARBONATESTjahjono Herawan, M. Rüsch Gen. KlaasLipase-catalyzed transesterifications-especially in a solvent-free medium-are important for industrial applications because such systems would have an enormous advantage by avoiding the problem of separation, toxicity and flammability of organic solvents. However, the organic solvent-free alcoholysis, especially methanolysis, does not give high conversions. The same problem also occurs when ethyl or methyl acetate are used as acyl acceptors. The main problems … …American Journal of Applied Sciences, Vol. 11, Issue 8 · 6 May 2014 · 6,798 accessesView PDFCite
Research ArticleOpen accessIn the abstractDegumming of Raw Silk Fabric with Help of Marine Extracellular ProteaseDas Sumana, Mathummal Sudarshan, Ashoke Ranjan Thakur, Shaon RayChaudhuriProtease secreting microbe was isolated and characterized on the basis of their morphological, biochemical, physiological and 16S rDNA based molecular properties. The extracellular protease was quantified and characterized. Protease was used for different time (4, 8, 12 and 24 h) at different temperature (RT and 37°C) for optimization of the degumming process for raw silk fabric with enzyme dosage (0.2-1 … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 1 · 18 Mar 2013 · 8,504 accessesView PDFCite
Research ArticleOpen accessIn the abstractBiotransformation Using Recombinant Cmp Sialic Acid Synthetase and α-2, 6-Sialyltran Sferase: Enzymatic Synthesis of SialosidesUlrike Hubl, Cynthia Sun, Shuguang Zhang et al.In this research, we successfully expressed recombinant CMP-sialic Acid Synthetase (CSS) from Neisseria meningitides and 2,6-Sialyltransferase (SAT) from Photobacterium damsela in E. coli BL21(DE3) fermented at a scale of up to 8 litres using individual plasmids pIRL-1 and pIRL-4b, respectively. After cell lysis with BugBuster, enzyme levels of 2U and 22U per litre were produced for CSS and SAT, respectively. … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 4 · 25 Dec 2012 · 8,860 accessesView PDFCite
Research ArticleOpen accessIn the abstractPapain, a Plant Enzyme of Biological Importance: A ReviewFlorence Mamboya, Ezekiel AmriPapain is a plant proteolytic enzyme for the cysteine proteinase family cysteine protease enzyme in which enormous progress has been made to understand its functions. Papain is found naturally in papaya (Carica papaya L.) manufactured from the latex of raw papaya fruits. The enzyme is able to break down organic molecules made of amino acids, known as polypeptides and thus … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 2 · 22 Jun 2012 · 44,738 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Dynamics Studies of Human β-GlucuronidaseIbrahim Ali Noorbatcha, Ayesha Masrur Khan, Hamzah Mohd SallehProblem statement: The enzyme β-glucuronidase is being used as a reporter molecule in the area of genetic engineering, as a component of prodrug therapy in cancer treatment and in the scouring process of cotton fabrics. However, a detailed understanding of the factors responsible for the stability and the activity of this enzyme is still not available. Molecular Dynamics (MD) simulations … …American Journal of Applied Sciences, Vol. 7, Issue 6 · 30 Jun 2010 · 7,619 accessesView PDFCite
Research ArticleOpen accessIn the abstractCharacteristics of a Novel Highly Thermostable and Extremely Thermophilic Alkalitolerant Amylase from Hyperthermophilic Bacillus Strain HUTBS71Farouk Al-Quadan, Hazem Akel, Rasha NatshiProblem statement: This study reported the purification and characterization of a novel highly thermostable alkaline amylase from a newly isolated Bacillus strain HUTBS71. Approach: The enzyme was purified using ammonium sulfate precipitation, ion exchange and gel filtration chromatography. Results: Maximum amylase activity (72 U mL-1) was obtained at 100°C after 10 min of incubation. The enzyme was purified 24 fold … …OnLine Journal of Biological Sciences, Vol. 9, Issue 3 · 30 Sep 2009 · 7,404 accessesView PDFCite
EditorialOpen accessIn the abstractNanotechnology: Engineering Materials for a Better 21st CenturyMeng Qiao, Xiyun Liu, Lei ZhangToday, we are living in the midst of a technological revolution, where nanotechnology is demonstrating a greater commitment to make people’s everyday lives better. The aim of nanotechnology is to create new materials with desired properties by engineering their nanoscale structures and components. One of the most important impacts of nanotechnology is in medical diagnosis and treatment. Cardiovascular diseases such … …Current Research in Nanotechnology, Vol. 5, Issue 1 · 26 Nov 2014 · 8,743 accessesView PDFCite