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150 articles for “ELISA”
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Research ArticleOpen accessIn the keywordsISOLATION AND DETECTION OF HELICOBACTER PYLORI FROM PATIENTS SUFFERING FROM PEPTIC ULCER USING BIOCHEMICAL TESTS AND MOLECULAR TECHNIQUESRuba S. Abu-Sbeih, Azmi D. Hawari, Dhia S. Hassawi, Hala I. Al-DaghistaniHelicobacter pylori is the causative agent of most cases of gastritis. There is no established gold standard for the diagnosis of H. pylori infection. A reliable diagnosis is crucial to confirm that eradication therapy has been successful. Eighty gastric biopsy and blood samples were obtained from fasting Jordanian patients with Esophago-Gastro-Doudenoscopy (EGD). Several diagnosis tests for H. pylori infections were … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 28 Mar 2014 · 8,183 accessesView PDFCite
Research ArticleOpen accessIn the keywordsSCREENING FOR CUCUMBER MOSAIC RESISTANT LINES FROM THE OVULE CULTURE DERIVED DOUBLE HAPLOID CUCUMBERSParichat Plapung, Sirisupaporn Khamsukdee, NutthaPotapohn, Prasartporn SmitamanaCMV is one of the major destructive viruses worldwide and commercial CMV resistant cucumber is very rare. Therefore we aimed to establish the ovule derived resistant lines used for the breeding program. Haploid plants of sixty-eight cucumber lines were successfully obtained by culturing the un pollinated ovaries harvested one day before an thesis on a modified MS medium supplemented with … …American Journal of Agricultural and Biological Sciences, Vol. 9, Issue 3 · 28 Feb 2014 · 7,903 accessesView PDFCite
Research ArticleOpen accessIn the keywordsSoluble CD59-Antigen Levels are Increased in Renal Disease PlasmaOluseyi Adewale VanderpuyeCluster of Differentiation antigen 59 (CD59) is a glycosyl phosphatidylinositol-linked protein that protects cells from damage by lytic terminal complexes of complement. Soluble forms of CD59 in serum have been little investigated in disease states. In the present study, by using a sandwich ELISA, reactivities with antibodies to CD59 were compared for sera and plasma from normal individuals and those … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 3 · 21 Sep 2013 · 6,944 accessesView PDFCite
Research ArticleOpen accessIn the keywordsIgG Subclass Variation of a Monoclonal Antibody Binding to Human Fc-Gamma ReceptorsRekha Patel, Krista K. Johnson, Bruce A. Andrien, Paul P. TamburiniThe importance of human Fc receptors in immune regulation is well known. Their role is critical not only in the recruitment of cellular effector functions but also in regulating the balance in the periphery between autoimmunity and tolerance. Despite their central importance, there is a dearth of literature on controlled numeric comparisons in affinities of antibody subclasses for gamma receptors. … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 3 · 21 Sep 2013 · 8,065 accessesView PDFCite
Research ArticleOpen accessIn the keywordsComparison of enzyme Immunoassay with Immunofluorescence Assay for the Diagnosis of Mycoplasma pneumoniae Respiratory Tract Infections in ChildrenOsama Mohammed Saed Abdul-WahabMycoplasma pneumoniae is a frequent cause of acute respiratory infections in both children and adults. Currently, diagnosis of M. pneumoniae infection will based principally on serology and the detection of IgM can provide an early and sensitive diagnosis in children. A variety of commercial immunodiagnostic assays, such as Indirect Enzyme-Linked Immunosorbent (ELISA) assay and Indirect Immunofluorescence (IFA), are now available … …American Journal of Immunology, Vol. 9, Issue 2 · 21 Jun 2013 · 7,412 accessesView PDFCite
Research ArticleOpen accessIn the keywordsA Seroepidemiological Survey of Hantavirus in Ilheus CountyMarcos Lazaro Moreli, Vivaldo Gomes da Costa, Flavia Rubia ParizHantaviral diseases are infections caused by a group of viruses that belong to the genus hantavirus and the family Bunyaviridae. In Brazil, several cases of hantavirus have been detected with the use of serological methods including immunofluorescence and Enzyme-Linked Immunosorbent Assay (ELISA). To date, 1.573 cases have been reported in several states across the country with a fatality rate of … …Current Research in Virology, Vol. 1, Issue 1 · 30 Aug 2012 · 13,382 accessesView PDFCite
Research ArticleOpen accessIn the keywordsInfluence of Heirloom Tomato Polyphenol Extracts on Expression of Inflammation Genes in BovineKwaku Gyenai, Nona Mikiashvili, Hamid Ismail, Mulumebet WorkuThe effect of tomato on diseases of economic importance continues to be of broad interest. To evaluate these relationships, the effect of crude extracts of Cherokee purple tomato on pro-inflammatory genes and cytokines were evaluated. Neutrophils from three Holstein Fresian cows were treated with Phosphate Buffered Saline (PBS) or E. coli O111-B4 Lipopolysaccharide (LPS). Treated cells were exposed to different … …American Journal of Animal and Veterinary Sciences, Vol. 7, Issue 3 · 25 Aug 2012 · 8,377 accessesView PDFCite
Research ArticleOpen accessIn the keywordsInterleukin-10, Mean Arterial Blood Pressure and Insulin Resistance in Normal PregnancyAbdolreza Sotoodeh Jahromi, Mohammad Reza Farjam, Mohammad Hassan Davami et al.Problem statement: The purpose of this study was to investigate whether serum of Interleukin-10 (IL-10) Concentration change during normal pregnancy and, if so, to relate these changes corresponding alterations in insulin resistance and blood pressure. Approach: This cross sectional study was carried out on 86 healthy pregnant women including 26, 23 and 37 individuals in the 1st, 2nd and 3rd … …OnLine Journal of Biological Sciences, Vol. 11, Issue 3 · 2 Dec 2011 · 7,815 accessesView PDFCite
Research ArticleOpen accessIn the keywordsSeroprevalence of Rubella Virus in Women with Spontaneous AbortionAbdolreza Sotoodeh Jahromi, Akbar Kazemi, Gita Manshoori et al.Problem statement: Recurrent abortion is a difficult medical problem happening in about 1-2% of fertile women. Rubella is of high public health importance owing to teratogenic effects that can lead to fetal death, miscarriage, stillbirth, or infants during the first 16 weeks of gestation. As there were not data about role this virus in abortion in this area of IRAN, … …American Journal of Infectious Diseases, Vol. 7, Issue 1 · 27 Apr 2011 · 8,732 accessesView PDFCite
Research ArticleOpen accessIn the keywordsAnti β2-Glycoprotein I Antibodies in Women with Recurrent Spontaneous AbortionAbdolreza Sotoodeh Jahromi, Mohammad Reza Farjam, Farideh Mogharrab et al.Problem statement: Antiphospholipid antibodies (aPL) are associated with repeated miscarriages and pregnancy complications, however their pathogenic mechanisms are still matter of research. Anti-β2 glycoprotein 1 (β2GPI) antibody is one of these aPL that its contribution to RSA risk remains poorly understood. There were not any data about role this auto-antibody in RSA in South of Iran. This study was conducted … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 7,787 accessesView PDFCite