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27 articles for “DPPH assay”

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Research ArticleOpen accessIn the abstractSeparation, Purification and Anti-aging Activity of Tobacco PolysaccharidesMengyue Huang, Jingwu Song, Shuaishuai Chang et al.To separate tobacco polysaccharides and investigate their antioxidant and anti-aging properties, crude tobacco polysaccharides were extracted from raw tobacco using hot water, followed by purification through DEAE-52 cellulose column chromatography. The molecular weight and monosaccharide composition of the isolated polysaccharides were analyzed using HPGPC and HPLC. The antioxidant activities of these tobacco polysaccharides were tested using DPPH, ABTS, hydroxyl radicals … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 1 · 15 Jan 2025 · 5,108 accessesView PDFCite
Research ArticleOpen accessIn the abstractAcetylation Process and Antioxidant Activity of a Polysaccharide from Loquat (Eriobotrya japonica) LeavesYiting Xue, Xiao Ding, Ze Zhang et al.Chemical modifications of polysaccharides can provide an opportunity to obtain new agents with possible therapeutic uses. In this study, a water-soluble polysaccharide extracted from Loquat Leaves (coded LLPs) was purified by the column chromatography, which was used to investigate its acetylation process by the acetic anhydride method. The Degree of Substitution (DS) was used as the index, the effect of … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 4 · 1 Jan 2021 · 7,059 accessesView PDFCite
Research ArticleOpen accessIn the abstractExtraction, Partial Identification and Bioactivities of Total Flavonoids from Carex meyeriana KunthHongbiao Wang, Penghui Wang, Xiudong Yang et al.To date, knowledge associated with extraction, chemical constituents and bioactivities of Total Flavonoids from Carex meyeriana Kunth (TFCMK) that remains unclear. Therefore, this paper was designed to optimize the ethanol reflux extraction method by response surface methodology and determine the chemical constituents by Liquid Phase-Mass Spectrometry (HPLC-MS); additionally, the bioactivities including antioxidant, antibacterial and hemolysis test were also explored. The … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 3 · 7 Aug 2019 · 7,525 accessesView PDFCite
Research ArticleOpen accessIn the abstractModulation of Aging in Yeast Saccharomyces cerevisiae by Roselle Petal Extract (Hibiscus sabdariffa L.)Sarima, Rika Indri Astuti, Anja MeryandiniCellular aging drives diseases. Free radicals are called the main cause of cellular aging. Antioxidants can protect the body from free radical attack by reducing its negative effects. An antioxidant source can be obtained from plants, such as roselle (Hibiscus sabdariffa. L). This research was conducted to analyze the ability of roselle petals extract to increase the life span of … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 1 · 6 Apr 2019 · 9,651 accessesView PDFCite
Research ArticleOpen accessIn the abstractComparative Extraction of Pectic and Polyphenols from Mexican Lime Pomace and BagasseDaniela Sanchez-Aldana, Cristobal Noe Aguilar, Guadalupe Virginia Nevarez-Moorillon, Juan Carlos Contreras EsquivelMexican lime bagasse and pomace are rich in pectin and they also represent an important source of value-added compounds such as polyphenols. Two different options for the combined recovery of pectin and phenolic compounds from Mexican lime bagasse and pomace, two byproducts of industrial lime processing, were developed. Conventional and microwave-assisted extraction methods were used. All pectic extracts presented a … …American Journal of Agricultural and Biological Sciences, Vol. 8, Issue 4 · 29 Oct 2013 · 7,811 accessesView PDFCite
Research ArticleOpen accessIn the abstractAnti-Oxidant and Enzyme-Inhibitory Potential of Marine StreptomycesT. Revathy, M. A. Jayasri, K. SuthindhiranMarine actinomycetes are potential source for the discovery of novel compounds and enzymes. Though extensive research on marine actinomycetes is underway globally, the actinomycetes research from Indian marine ecosystem is unexplored and understudied. Hence, the present research is focussed on the screening of bioactive compounds from marine actinomycetes isolated from Indian coastal region. This study is designed to determine the … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 3 · 21 Sep 2013 · 7,242 accessesView PDFCite
Research ArticleOpen accessIn the abstractAntioxidant Capacity and Amino Acid Profiles of Egg TofuMaizura Murad, Aminah Abdullah, Wan Aida Wan MustaphaTofu contains high quality protein source and antioxidant which could reduce risk of cancer. This research aims to determine the effect of soymilk and egg ratios on the antioxidant capacity, daidzein and genistein content and amino acid profiles of egg tofu. Egg tofu was prepared using soymilk and fresh egg in ratios of 1:1, 2:1, 3:1 and 4:1. Glucono-Delta-Lactone (GDL) … …American Journal of Applied Sciences, Vol. 10, Issue 11 · 9 Sep 2013 · 8,924 accessesView PDFCite
Research ArticleOpen accessIn the abstractAntioxidant Potential in Lipophilic and Hydrophilic Extracts from Medicinal Herbs (Salvia officinalis and Echinacea angustifolia). A Comparison Between Assays Based on Electron Paramagnetic Resonance and SpectrophotometryFlavia Navari-Izzo, Riccardo Izzo, Mike F. Quartacci et al.Recently, detection of antioxidant activity of plant extracts has caught the attention of researchers. Determination of antioxidant activity is usually performed using stable free radicals. A number of assays is available for screening the antioxidant activity of food constituents, but few of these methods are applicable to lipophilic substances. EPR is the analytical technique that directly measures free radicals making … …American Journal of Agricultural and Biological Sciences, Vol. 7, Issue 4 · 6 Nov 2012 · 9,013 accessesView PDFCite
Research ArticleOpen accessIn the abstractGermination Time Dependence of Bioactive Compounds and Antioxidant Activity in Germinated Rough Rice (Oryza sativa L.)Anuchita Moongngarm, Ekkalak KhomphiphatkulProblem statement: Germinated rice has been recognized as a functional food and its health benefits. However, most related studies were on germinated brown rice but our previous study indicated that germination of rough rice was an effective method to obtain high concentrations of bioactive compounds. Germination time is one of the most important factors affecting the level of biochemical compositions … …American Journal of Applied Sciences, Vol. 8, Issue 1 · 31 Jan 2011 · 8,670 accessesView PDFCite
Research ArticleOpen accessIn the abstractDetermination and Evaluation of Antioxidative Activity in Red Dragon Fruit (Hylocereus undatus) and Green Kiwi Fruit (Actinidia deliciosa)Normala Halimoon, Mardhiah Hayati Abdul HasanProblem statement: Dragon fruit or pitahaya (Hylocereus undatus), is believed to be a healthy source of vitamins, fiber and antioxidants, especially the red-fleshed varieties which contain lycopene. Approach:Compared to green kiwi fruit (Actinidia deliciosa), which already known contain high antioxidant activity. Results:The antioxidant capacity of Hylocereus undatus (H. undatus) and Actinidia deliciosa (A. deliciosa) in three different solvent extraction; ethanol, … …American Journal of Applied Sciences, Vol. 7, Issue 11 · 8 Nov 2010 · 9,160 accessesView PDFCite