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39 articles for “Clone”
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Research ArticleOpen accessIn the abstractSCREENING FOR CUCUMBER MOSAIC RESISTANT LINES FROM THE OVULE CULTURE DERIVED DOUBLE HAPLOID CUCUMBERSParichat Plapung, Sirisupaporn Khamsukdee, NutthaPotapohn, Prasartporn SmitamanaCMV is one of the major destructive viruses worldwide and commercial CMV resistant cucumber is very rare. Therefore we aimed to establish the ovule derived resistant lines used for the breeding program. Haploid plants of sixty-eight cucumber lines were successfully obtained by culturing the un pollinated ovaries harvested one day before an thesis on a modified MS medium supplemented with … …American Journal of Agricultural and Biological Sciences, Vol. 9, Issue 3 · 28 Feb 2014 · 7,903 accessesView PDFCite
Research ArticleOpen accessIn the abstractSECURE COMMUNICATION PROTOCOL FOR PROTECTING COMPUTATION RESULT OF FREE ROAMING MOBILE AGENTG. Geetha, C. JayakumarMobile agent plays an important role in developing applications of open, distributed and mixed environments, such as the internet. Mobile agent or mobile software agent is piece of software that can operate autonomously to accomplish user assigned task. To explain more, mobile agent is the process which can migrate to hosts autonomously. As an agent travels to do execution in … …Journal of Computer Science, Vol. 10, Issue 7 · 19 Feb 2014 · 6,800 accessesView PDFCite
Research ArticleOpen accessIn the abstractResponses in Nitrogen Mass and Nitrogen Metabolism of Wild Sugarcane (Saccharum spontaneum L.) UV-B Radiation Under Field ConditionsYanqun Zu, Yuan Li, Haiyun Wang, Yongmei HeField experiments were conducted to evaluate effects of enhanced UV-B radiation (280-315 nm) on intraspecific responses in nitrogen mass and nitrogen metabolism of six wild sugarcane (Saccharum spontaneum L.) clones. The clones were collected from original sites with different altitudes (4-1780 m above sea-level) and latitudes (18-38°N). The supplemental UV-B radiation was 5.00 kJ⋅m-2, simulating a depletion of 20% stratospheric … …American Journal of Environmental Sciences, Vol. 9, Issue 6 · 7 Jan 2014 · 6,656 accessesView PDFCite
Research ArticleOpen accessIn the abstractGeneration of Anti-platelet Autoantibody During Dengue Virus InfectionKao-Jean Huang, Yee-Shin Lin, Hsiao-Sheng Liu et al.Dengue virus infection causes dengue fever, Dengue Hemorrhagic Fever (DHF) and Dengue Shock Syndrome (DSS). Thrombocytopenia is common in dengue fever and is always found in DHF/DSS. The pathogenesis of thrombocytopenia is poorly understood. To further understand the relationship between anti-dengue virus antibody and anti-platelet antibody, we generated monoclonal anti-dengue virus antibodies from the dengue virus infected mice that developed … …American Journal of Infectious Diseases, Vol. 4, Issue 1 · 31 Mar 2008 · 7,535 accessesView PDFCite
Research ArticleOpen accessIn the abstractGeneration of Five New Musa Hybrids With Resistance To Black Sigatoka and High YieldRicardo Hernández, Teresa Ramírez, Juan Carlos Noa-Carrazana et al.The ability to identify genetic variation is indispensable to effective management and use of genetic resources. This work is the first approach concerning to generation and genetic differentiation of new Musa hybrid lines obtained in INIVIT (Cuba), crossing Musa acuminata male diploid (AA) x female triploid (ABB), genotypes selected by their resistant character to pests and diseases. From the obtained … …American Journal of Agricultural and Biological Sciences, Vol. 2, Issue 2 · 30 Jun 2007 · 8,811 accessesView PDFCite
Research ArticleOpen accessIn the abstractExpression and Induction Optimization of Mucor Circinelloides Lipase in Escherichia ColiYueping Yang, Yao Zhang et al.Microbial lipases serve as versatile biocatalysts for modifying lipid substrates, enabling diverse biotechnological applications spanning food technology, renewable energy production, and pharmaceutical sectors. Genomic analysis of Mucor circinelloides WJ11 revealed multiple lipase-encoding genes, with WJ_23 successfully cloned and heterologously expressed in Escherichia coli. Since the lipase expression was mainly concentrated in the cell pellets, appropriate induction strategies had been investigated … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 2 · 13 Jun 2025 · 5,982 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Development Technique of Side and Budwood Grafting Improving Production of Cocoa in Mamuju Regency West Sulawesi, IndonesiaIda SuryaniThe one of human effort increasing productivity and high quality of cocoa is rehabilitating plant using plant materials with high production and resistant clones to Cocoa Pod Borer. The common methods using side and budwood grafting technique. The research objectives is to compare two grafting technology for old cocoa plant, community empowerment in developing side grafting of cocoa plants by … …OnLine Journal of Biological Sciences, Vol. 21, Issue 2 · 7 Apr 2021 · 6,949 accessesView PDFCite
Research ArticleOpen accessIn the abstractInfluence of Tags on the Binding Affinity of Acyl-CoA Binding ProteinJizhong Han, Yunlong Sun, Yu Chen et al.Tagged fusion proteins are frequently employed for protein purification methods, but their effects on protein function and binding affinity are rarely studied. Here we expressed recombinant protein Acyl-CoA Binding Protein (ACBP) cloned from the full-length cDNA of Aspergillus oryzae and Saccharomyces cerevisiae. ACBP was expressed in Escherichia coli fused to a Maltose-Binding Protein (MBP) and Histidine-tag fusion. Recombinant ACBP was … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 1 · 23 Jan 2019 · 9,097 accessesView PDFCite
Research ArticleOpen accessIn the abstractAntimicrobial Resistance of Campylobacter spp. and Arcobacter butzleri from Pets in MalaysiaMohammed Dauda Goni, Abdinasir Yusuf Osman, Saleha Abdul Aziz et al.The emergence of antimicrobial resistance in pets is not well understood and methods of surveillance are only beginning to be established in a few countries. The consequence of antimicrobial resistant Campylobacter and Arcobacter butzleri to public health is due to the propensity of the bacteria to swiftly acquire and disseminate resistance gene. Thus, making way for the emergence of new … …American Journal of Animal and Veterinary Sciences, Vol. 13, Issue 4 · 29 Nov 2018 · 7,690 accessesView PDFCite
Research ArticleOpen accessIn the abstractConstruction of Recombinant Bioleaching Bacteria Thiobacillus ferrooxidans with Fluoride-Resistant ActivityYun-Chang Cao, Hong-Bo Wen, Nan Hu et al.Thiobacillus ferrooxidans is an important bioleaching bacterium, widely used in leaching, recovery of low-grade metals and environmental bioremediation. In bio-metallurgy industry, fluoride existing in some hard rock uranium deposits in south China severely affects the bacteria viability and inhibit leaching reproduction. In this study, the flr-4 gene that has strong resistance to fluorine ion in Caenorhabditis elegans, was cloned into … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 2 · 23 May 2018 · 11,341 accessesView PDFCite