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Research ArticleOpen accessIn the abstractDetermination of the Presence of Huanglongbing in Seeds and Movement of the Pathogen in Citrus reticulataHajivand Shokrollah, Thohirah Lee Abdullah, Kamaruzaman Sijam, Siti Nor Akmar AbdullahProblem statement: Huanglongbing (HLB) also known as citrus greening disease is a fastidious phloem-inhabiting bacterium in the genus Candidatus Liberibacter. Using universal primers, the 16S ribosomal DNA sequence of three strains of the bacterium were obtained by PCR. However there is very little information on seed transmission and HLB pathogen movement to find a way for control or reduce the … …American Journal of Applied Sciences, Vol. 6, Issue 6 · 30 Jun 2009 · 8,088 accessesView PDFCite
Research ArticleOpen accessIn the abstractImproving a PCR-Based Method for Identification of Ralstonia solanacearum in Natural Sources of West MalaysiaReza Khakvar, Kamaruzaman Sijam, Wong Mui Yun et al.Ralstonia solanacearum, a soil-borne plant pathogen, causes lethal wilting disease of more than 200 plants worldwide. This very wide host range covers both monocots and dicots, extending from annual plants to trees and shrubs. Although generally it’s considered as a plant pathogen, R. solanacearum behaves primarily as a saprophytic bacterium able to survive for long periods of time in various … …American Journal of Agricultural and Biological Sciences, Vol. 3, Issue 2 · 30 Jun 2008 · 9,469 accessesView PDFCite
Research ArticleOpen accessIn the abstractChamaemelum nobile L. Aqueous Extract Represses Endogenous Glucose Production and Improves Insulin Sensitivity in Streptozotocin-induced Diabetic MiceLemhadri Ahmed, Remy Burcelin, Thierry Sulpice, Mohamed EddouksThe present study was undertaken in order to evaluate the possible mechanisms of action involved in the hypoglycaemic activity of the aqueous extract of Chamaemelum nobile (CN) (20 mg kg-1). This study was carried out in multi low-dose streptozotocin-induced (MLDS) diabetic mice. Hypoglycaemic effect of CN was studied after both single and repeated oral administration of CN aqueous extract (20 … …American Journal of Pharmacology and Toxicology, Vol. 2, Issue 3 · 30 Sep 2007 · 7,255 accessesView PDFCite
Research ArticleOpen accessIn the abstractAFLPs in Warmblood Sporting Horses: Genetic Correlation with Height at the WithersV. Sanchez, C. Lemus, A. Inchaustegui et al.The Mexican army produces Warmblood horses for jumping proposes. However, breeding is based on phenotype and stallions are selected stepwise until the end of growth, representing a high care and maintenance cost. In this work using 64 selective primer combinations and AFLP technology we identified specific molecular markers related with height at the withers measurements of Warmblood horses. AFLPs generated … …American Journal of Infectious Diseases, Vol. 3, Issue 2 · 30 Jun 2007 · 6,753 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Variation and Relationship Between Turkish Flint Maize Landraces by RAPD MarkersA. OkumusA comparative characterization of 17 flint maize landraces (Zea mays L.) was carried out using RAPD markers. Fourteen primers giving reliable and consistent polymorphic bands amplified 125 fragments (89%) with an average of 8.90 fragment per primer. Genetic variation in 17 maize landraces was characterized based on dissimilarity matrix by UPGMA dendogram which has no association into distinct grouping with … …American Journal of Agricultural and Biological Sciences, Vol. 2, Issue 2 · 30 Jun 2007 · 7,977 accessesView PDFCite
Research ArticleOpen accessIn the abstractToll-Like Receptor (TLR) 2 and TLR4 Gene Expression in Canine HeartA. Linde, F. Blecha, T. MelgarejoToll-like receptors (TLRs) are archetypal pattern recognition receptors of immediate importance for an efficacious innate immune response. TLRs exhibit marked differential tissue activity and their levels within a discrete cell type can be highly dynamic. Of 13 known mammalian paralogues, three TLRs have been identified in the dog. Although cardiac TLR expression has been reported in other species, this study … …American Journal of Animal and Veterinary Sciences, Vol. 2, Issue 1 · 31 Mar 2007 · 6,921 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Characterization of Black Bengal and Jamuna Pari Goat Breeds By RAPD MarkersM.A. Rahman, S.M.M. Rahman, M.A. Jalil et al.This experiment was conducted in the Genetics, Breeding and Reproductive Biotechnology Laboratory under Goat and Sheep Production Research Division, Bangladesh Livestock Research Institute (BLRI), Saver, Dhaka, Bangladesh. DNA was extracted from 14 goat breeds and the extracted DNA was observed by gel electrophoresis. Eight goat specific primers were synthesized by ASM-800 DNA synthesizer and screened in the study and all … …American Journal of Animal and Veterinary Sciences, Vol. 1, Issue 2 · 31 Dec 2006 · 9,584 accessesView PDFCite
Research ArticleOpen accessIn the abstractLarge Scale Production and Characterization of Anti-Human IgG Monoclonal Antibody in Peritoneum of Balb/c MICEB. Baradaran, J. Majidi, Z. M. Hassan, J. AbdolalizadehMonoclonal antibodies are key reagents that are used in biomedical researches, diagnosis of immunodeficiency diseases such as IgG subclasses deficiency and treatment of diseases like infections and cancers. For large scale production of monoclonal antibody, hybridoma cells that produce monoclonal antibody against human IgG were injected into the peritoneum of the Balb/c mice which have previously been primed with 0.5 … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 4 · 31 Dec 2005 · 7,089 accessesView PDFCite