163 results for “characterization analysis”

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Research ArticleOpen accessAll words, across the recordIdentification and functional characterization of a novel lysine-rich protein from Entamoeba histolyticaAlka Mehra, Sudha Bhattacharya, Alok BhattacharyaIn an attempt to understand the role of genes with no known function, in the protozoan parasite Entamoeba histolytica, an analysis of the proteome was carried out and a Lysine-Rich protein (KRP) was identified for further study. This protein has a modular structure with four domains and was found to be expressed and localized to the cell surface of E. … …American Journal of Biochemistry and Biotechnology, Vol. 3, Issue 4 · 31 Dec 2007 · 7,707 accessesView PDF Cite

Research ArticleOpen accessAll words, across the recordMolecular Characterization of Soybean Mosaic Virus NIa Protein and its Processing Event in Bacterial ExpressionBong K. Choi, Jae S. Park, Hye J. Ahn et al.Soybean mosaic virus (SMV)-CN18 is an Rsv resistance-breaking (RB) isolate to overcome soybean resistance genes Rsv1, Rsv3 and Rsv4. The aim of this study was to characterize nuclear inclusion protein a (NIa protein) of RB isolate at the molecular level and demonstrate its processing into genome-linked protein (VPg) and NIa-Pro domains in Esherichia coli containing a bacterial expression pET vector … …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 4 · 31 Dec 2006 · 6,902 accessesView PDF Cite

Research ArticleOpen accessAll words, across the recordUltrastructural and Immunological Characterization of Hepatitis C Core Protein-DNA Plasmid ComplexesNelson Acosta-Rivero, Yaraima Aguilera, Viviana Falcon et al.Recently, it has been shown that a truncated HCV core (HCcAg) variant, covering the first 120 aa (HCcAg.120), interacts with plasmid DNA vaccine (pIDKE2), encoding the HCV structural proteins (HCcAg, E1 and E2). In the present work, HCcAg.120-pIDKE2 complexes, forming heterogeneous packaged structures, were visualized using a negative stain/rotary shadow technique. Interestingly, 72 hours after intramuscular injection of HCcAg.120-pIDKE2 complexes … …American Journal of Immunology, Vol. 2, Issue 3 · 30 Sep 2006 · 6,743 accessesView PDF Cite