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Research ArticleOpen accessIn the abstractMolecular Characterization of Opium Poppy (Papaver somniferum) GermplasmHari Shanker Acharya, Vimal SharmaProblem statement: The medicinal value of opium poppy (2n = 22, Papaver somniferum L.) is due to presence of more than two dozen alkaloids. The genetic origin and diversity of cultivars used in the breeding collection is not fully known. To evaluate the genetic diversity of the 24 germplasm currently cultivated in the state to provide genetic information about heterogeneity, … …American Journal of Infectious Diseases, Vol. 5, Issue 2 · 30 Jun 2009 · 8,096 accessesView PDFCite
Research ArticleOpen accessIn the abstractIsolation and Characterization of Potential Probiotic Escherichia coli Strains from Rat Faecal SamplesPrasant Kumar, S. Ferzin, S. Chintan, G. Naresh KumarProblem statement: Gastrointestinal disorders mainly diarrhea is the most common cause of morbidity and mortality among infants and children in the developing countries occurs due to infection by enteropathogens. Ccontrol of these pathogens could be achieved by nonpathogenic Escherichia coli producing bacteriocins. Approach: Primary aim of this study was to isolate E. coli strains which inhibit enteropathogens. For this purpose, … …American Journal of Infectious Diseases, Vol. 5, Issue 2 · 30 Jun 2009 · 8,417 accessesView PDFCite
Research ArticleOpen accessIn the abstractAssessment of Site Specific Mutational Effect on Transcription Initiation at Escherichia coli PromoterS. Kannan, M. Krishnan, S. Sundarraj et al.Problem statement: It is widely accepted thought that the weak promoters control the RNA synthesis and play regulatory role in complex genetic networks in bacterial system. An experiment had been designed to address whether mutations in the -16/-17 region affect the rate of transcription at an activator-independent promoter in E. coli or not? Approach: The aim of this study was … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 2 · 30 Jun 2009 · 7,555 accessesView PDFCite
Research ArticleOpen accessIn the abstractDetermination of the Presence of Huanglongbing in Seeds and Movement of the Pathogen in Citrus reticulataHajivand Shokrollah, Thohirah Lee Abdullah, Kamaruzaman Sijam, Siti Nor Akmar AbdullahProblem statement: Huanglongbing (HLB) also known as citrus greening disease is a fastidious phloem-inhabiting bacterium in the genus Candidatus Liberibacter. Using universal primers, the 16S ribosomal DNA sequence of three strains of the bacterium were obtained by PCR. However there is very little information on seed transmission and HLB pathogen movement to find a way for control or reduce the … …American Journal of Applied Sciences, Vol. 6, Issue 6 · 30 Jun 2009 · 8,088 accessesView PDFCite
Research ArticleOpen accessIn the abstractImproving a PCR-Based Method for Identification of Ralstonia solanacearum in Natural Sources of West MalaysiaReza Khakvar, Kamaruzaman Sijam, Wong Mui Yun et al.Ralstonia solanacearum, a soil-borne plant pathogen, causes lethal wilting disease of more than 200 plants worldwide. This very wide host range covers both monocots and dicots, extending from annual plants to trees and shrubs. Although generally it’s considered as a plant pathogen, R. solanacearum behaves primarily as a saprophytic bacterium able to survive for long periods of time in various … …American Journal of Agricultural and Biological Sciences, Vol. 3, Issue 2 · 30 Jun 2008 · 9,469 accessesView PDFCite
Research ArticleOpen accessIn the abstractChamaemelum nobile L. Aqueous Extract Represses Endogenous Glucose Production and Improves Insulin Sensitivity in Streptozotocin-induced Diabetic MiceLemhadri Ahmed, Remy Burcelin, Thierry Sulpice, Mohamed EddouksThe present study was undertaken in order to evaluate the possible mechanisms of action involved in the hypoglycaemic activity of the aqueous extract of Chamaemelum nobile (CN) (20 mg kg-1). This study was carried out in multi low-dose streptozotocin-induced (MLDS) diabetic mice. Hypoglycaemic effect of CN was studied after both single and repeated oral administration of CN aqueous extract (20 … …American Journal of Pharmacology and Toxicology, Vol. 2, Issue 3 · 30 Sep 2007 · 7,255 accessesView PDFCite
Research ArticleOpen accessIn the abstractAFLPs in Warmblood Sporting Horses: Genetic Correlation with Height at the WithersV. Sanchez, C. Lemus, A. Inchaustegui et al.The Mexican army produces Warmblood horses for jumping proposes. However, breeding is based on phenotype and stallions are selected stepwise until the end of growth, representing a high care and maintenance cost. In this work using 64 selective primer combinations and AFLP technology we identified specific molecular markers related with height at the withers measurements of Warmblood horses. AFLPs generated … …American Journal of Infectious Diseases, Vol. 3, Issue 2 · 30 Jun 2007 · 6,753 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Variation and Relationship Between Turkish Flint Maize Landraces by RAPD MarkersA. OkumusA comparative characterization of 17 flint maize landraces (Zea mays L.) was carried out using RAPD markers. Fourteen primers giving reliable and consistent polymorphic bands amplified 125 fragments (89%) with an average of 8.90 fragment per primer. Genetic variation in 17 maize landraces was characterized based on dissimilarity matrix by UPGMA dendogram which has no association into distinct grouping with … …American Journal of Agricultural and Biological Sciences, Vol. 2, Issue 2 · 30 Jun 2007 · 7,977 accessesView PDFCite
Research ArticleOpen accessIn the abstractToll-Like Receptor (TLR) 2 and TLR4 Gene Expression in Canine HeartA. Linde, F. Blecha, T. MelgarejoToll-like receptors (TLRs) are archetypal pattern recognition receptors of immediate importance for an efficacious innate immune response. TLRs exhibit marked differential tissue activity and their levels within a discrete cell type can be highly dynamic. Of 13 known mammalian paralogues, three TLRs have been identified in the dog. Although cardiac TLR expression has been reported in other species, this study … …American Journal of Animal and Veterinary Sciences, Vol. 2, Issue 1 · 31 Mar 2007 · 6,921 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Characterization of Black Bengal and Jamuna Pari Goat Breeds By RAPD MarkersM.A. Rahman, S.M.M. Rahman, M.A. Jalil et al.This experiment was conducted in the Genetics, Breeding and Reproductive Biotechnology Laboratory under Goat and Sheep Production Research Division, Bangladesh Livestock Research Institute (BLRI), Saver, Dhaka, Bangladesh. DNA was extracted from 14 goat breeds and the extracted DNA was observed by gel electrophoresis. Eight goat specific primers were synthesized by ASM-800 DNA synthesizer and screened in the study and all … …American Journal of Animal and Veterinary Sciences, Vol. 1, Issue 2 · 31 Dec 2006 · 9,584 accessesView PDFCite