Sign inSubmit
Tips: “quotes” for a phrase · author:Lee · a DOI goes straight to its article

161 articles for “prime”
Set a search alert
Research ArticleOpen accessIn the abstractDetection of Listeria monocytogenes in Ready-to-Eat Meat ProductsAsma M.M.A. Abdelgadir, Kunwar K. Srivastava, P. Gopal ReddyProblem statement: The objective of this study was to develop a rapid and sensitive method for the detection of Listeria monocytogenes and to screen large number of ready-to-eat (RTE) meat samples. Approach: A total of 250 fresh RTE meat samples of different varieties (chicken, turkey, beef, pork and cold cuts) were purchased from local grocery stores. Results: From the 250 … …American Journal of Animal and Veterinary Sciences, Vol. 4, Issue 4 · 30 Jan 2012 · 7,427 accessesView PDFCite
Research ArticleOpen accessIn the abstractStudy and Identification of Insulin-Like Growth Factor-I Gene Polymorphisms in Zel Sheep PopulationSeyed Mohsen Kazemi, Cyrus Amirinia, Hossein Emrani, Shahaboddin GharahveysiProblem statement: The IGFs play an important role in regulating somatic growth according to nutritional conditions. Polymorphisms of IGF gene are reported to be significantly associated with many traits. Approach: In these study 142 samples of DNA from Zel sheep were used for detecting the polymorphisms of promoter region of the Insulin-like Growth Factor-1 (IGF-1) gene. Therefore DNA extracted from … …American Journal of Animal and Veterinary Sciences, Vol. 6, Issue 4 · 13 Jan 2012 · 7,745 accessesView PDFCite
Research ArticleOpen accessIn the abstractOccurrence of Feline Immunodeficiency Virus and Feline Leukemia Virus Infection in CatsFabiana Alves, Daniela de Souza Rajao, Helen Lima Del Puerto et al.Problem statement: The objective of the present study was to evaluate occurrence of the Feline Immunodeficiency Virus (FIV) and the Feline Leukemia Virus (FeLV) infection in asymptomatic domestic cats in the metropolitan region of Belo Horizonte, Minas Gerais, using the Polymerase Chain Reaction (PCR) for FIV detection and SNAP Combo Plus for FeLV and FIV detection. Approach: Blood samples were … …American Journal of Animal and Veterinary Sciences, Vol. 6, Issue 3 · 2 Nov 2011 · 8,721 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Variability in Rhizoctonia solani Isolated from Vitis vinifera Based on Amplified Fragment Length PolymorphismAmparo Meza-Moller, Martin Esqueda, Felipe Sanchez-Teyer et al.Problem statement: Rhizoctonia solani is a potential grapevine pathogen. In order to develop effective methods of control, it is necessary to document its genetic diversity. Approach: The objective of this study was to analyze the genetic variability of R. solani isolated from the rhizosphere of ungrafted V. vinifera var. perlette seedless planted in Sonora, Mexico using Amplified Fragment Length Polymorphism … …American Journal of Agricultural and Biological Sciences, Vol. 6, Issue 3 · 4 Aug 2011 · 8,479 accessesView PDFCite
Research ArticleOpen accessIn the abstractUsing Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction of Barbodes spp. in ThailandKeeravit Petjul, Aphidech Sangdee, Bungorn Thaewnon-ngiwProblem statement: The genetic diversity of Barbodes species in Thailand is presently unavailable. The objectives of this study were to study external morphology and genetic diversity using Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) of three Barbodes species; Barbodes altus, B. gonionotus and Barbodes sp. Approach: Two of twelve screened decanucleotide primers (OPA07 and UBC122), were selected for the genetic … …OnLine Journal of Biological Sciences, Vol. 11, Issue 1 · 6 Jul 2011 · 8,089 accessesView PDFCite
Research ArticleOpen accessIn the abstractCharacterization of the Genetic Diversity of Peach Cultivars in Taif by RAPD-PCRMohamed A. Nagaty, Salah El-Din El-Assal, Mahmoud M. RifaatProblem statement: Peach genotypes from the Kingdom of Saudi Arabia were not adequately characterized at the biological and molecular levels. This study was carried out to characterize nine peach cultivars growing in Taif (KSA) at the biological and molecular levels. Approach: For the nine peach cultivars studied, flowering and fruiting indices were determined and eight, 10-base primers were used to … …American Journal of Applied Sciences, Vol. 8, Issue 7 · 24 Jun 2011 · 7,279 accessesView PDFCite
Research ArticleOpen accessIn the abstractEarly Diagnosis of Breast Cancer using Molecular, Biochemical and Pathological MarkersSalah El-Din El-Assal, Adel A. El-Tarras, Samir M. Abd-allaProblem statement: Laboratory diagnosis of breast cancer in most of the hospitals has traditionally been performed using cell culture and the direct hormone receptor assay, which are money and time consuming. Approach: This study was performed in order to direct the attention toward increasing the efficiency of early diagnosis in clinical laboratories at the western region of KSA and Egypt … …American Journal of Applied Sciences, Vol. 8, Issue 1 · 31 Jan 2011 · 8,060 accessesView PDFCite
Research ArticleOpen accessIn the abstractEvaluation of Nested PCRs targeting the BI and SAG2 Genes For Detection of Toxoplasma gondii Genome in Aqueous Humor From HIV Positive Toxoplasma Retinochoroiditis Patients in a Tertiary Eye HospitalB. Mahalakshmi, K.L. Therese, R. Kirthika et al.Abstract: Problem statement: To evaluate nPCR targeting B1 gene (primer set 1) with 3 other nPCRs targeting B1 gene (primer set 2), 3' end of SAG2 and 5' end of SAG2 gene, for detection of Toxoplasma gondii (T. gondii) genome on AH from HIV positive TRC patients. Approach: DNA extracted from AH of 12 TRC patients and 12 controls (patients … …Current Research in Medicine, Vol. 1, Issue 2 · 31 Dec 2010 · 6,887 accessesView PDFCite
Research ArticleOpen accessIn the abstractCloning of the Endoglucanase Gene from a Bacillus amyloliquefaciens PSM 3.1 in Escherichia coli Revealed Catalytic Triad Residues Thr-His-GluZeily Nurachman, Sari Dewi Kurniasih, Ferra Puspitawati et al.Problem statement: An Indonesian marine bacterial isolate, Bacillus amyloliquefaciens PSM 3.1 was isolated for hydrolyzing cellulose. A 1500-bp nucleotide fragment was amplified from the chromosomal DNA by the use of primers directed against the conserved sequence of Bacilli endoglucanase genes obtained from GenBank. Approach: The fragment was cloned and expressed in Escherichia coli. Results: The endoglucanase gene (eglII gene) had … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 9,727 accessesView PDFCite
Research ArticleOpen accessIn the abstractSome Effects of Abiotic Stress on Infection of Dyer’s Woad (Isatis tinctoria L.) by Puccinia thlaspeos C. Schub.: Implications for Biological ControlElizabeth Thomas, Bradley Ray KroppProblem statement: The rust pathogen, Puccinia thlaspeos, is being studied as a potential biocontrol agent for the noxious weed dyer’s woad. Although its disease etiology is well understood, very little has been learned about the effect of environmental stresses on infection. Approach: Dyer’s woad plants were exposed to different levels of oxidative stress, salinity stress, osmotic stress, dehydration, and cold … …American Journal of Agricultural and Biological Sciences, Vol. 6, Issue 1 · 15 Dec 2010 · 8,719 accessesView PDFCite