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177 results for “isolation”
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Research ArticleOpen accessIn the abstractCELL DETACHMENT BY PROLYL-SPECIFIC ENDOPEPTIDASE FROM WOLFIPORIA COCOSNicole Mika, Katharina Cierpka, Michaela C. Lange et al.As requirements for Advanced Therapy Medicinal Product (ATMP) production differ from other production processes (e.g., therapeutic protein production), cell detachment is often a crucial step for the process success. In most cases, cell detachment is done enzymatically. Although many peptidases are established in cell culture in R&D, e.g., Trypsin as gold standard, many of them seem to be unsuitable in … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 8 Feb 2014 · 8,159 accessesView PDFCite
Research ArticleOpen accessIn the abstractPHENOTYPING, VIRULENCE CHARACTERISTICS OF AEROMONAS SPECIES AND THE EFFECTS OF ESSENTIAL PLANT OILS AS ANTIMICROBIAL AGENTS AGAINST PATHOGENIC ISOLATES FROM DIFFERENT SOURCESAhmed M.A. Mansour, Hoda M. Zaki, Nibal A. Hassan, Nihad A.M. El-NasharAeromonas species are increasingly recognized as enteric pathogens. Faecal samples from 20 cow, 45 sheep; 60 goat and 60 camels were examined for the presence of Aeromonas species, which was also sought in the available drinking water (55 well water and 52 drinking chlorinated tap water were also examined). Aeromonas species was isolated more frequently from goats (21.7%) than from … …American Journal of Infectious Diseases, Vol. 10, Issue 1 · 18 Jan 2014 · 8,024 accessesView PDFCite
Research ArticleOpen accessIn the abstractMOLECULAR CHARACTERIZATION AND IMMUNOPROTECTIVE ACTIVITY OF CAPSULAR POLYSACCHARIDE OF KLEBSIELLA PNEUMONIAE ISOLATED FROM FARM ANIMALS AT TAIF GOVERNORATEAhmed M.A. Mansour, Hoda M. Zaki, Nibal A. Hassan, Abdulrahman A. Al-HumianyKlebsiella pneumoniae is a Gram-negative enterobacterium that has historically been and currently remains, a significant cause of human disease and several kinds of infections in animals. In the present work, trials for the isolation of Klebsiella pneumoniae from diseased and apparently healthy farm animals (cows, sheep, goats and camels) were done for recognition of Klebsiella pneumoniae subspecies. It was noticed … …American Journal of Infectious Diseases, Vol. 10, Issue 1 · 6 Jan 2014 · 7,942 accessesView PDFCite
Research ArticleOpen accessIn the abstractACCURATE AND FAST PUPIL LOCALIZATION USING CONTRAST STRETCHING, SEED FILLING AND CIRCULAR GEOMETRICAL CONSTRAINTSIman A. Saad, Loay E. George, Ahmad A. TayyarIris segmentation is the most contested issue in the iris recognition system, since noise and poor image quality can significantly affect accuracy of iris localization stage. Therefore, very careful attention has to be paid for the segmentation process if only an accurate result is expected. This study presents a new method for precise pupil detection capable of handling the unconstrained … …Journal of Computer Science, Vol. 10, Issue 2 · 16 Nov 2013 · 7,247 accessesView PDFCite
Research ArticleOpen accessIn the abstractENERGY EFFICIENT AND INTERFERENCE AWARE PROVISIONING IN VIRTUALIZED SERVER CLUSTER ENVIRONMENTMohan Raj Velayudhan Kumar, Shriram RaghunathanIT service providers, employ server virtualization as a main building block to improve system utilization, improve system manageability, reduce operational costs which includes energy consumption driving economies of scale with shared resources. Virtualization enables co-locating and efficient assignments of virtual servers within the bounds of limited number of heterogeneous physical servers, with Virtual Machines (VM) sharing the limited physical server … …Journal of Computer Science, Vol. 10, Issue 1 · 12 Nov 2013 · 6,696 accessesView PDFCite
Research ArticleOpen accessIn the abstractAnalysis of Financial Markets Integration of Iran Within the Middle East and with the Rest of the WorldParinaz EzzatiIt is universally argued that Iran’s financial markets are effectively isolated from the rest of the world. However, in the last three years, privatization increased in Iranian financial markets as well as capitalization, Foreign Direct Investment (FDI) and equity prices, albeit with suspicion of reaching the bubble level. Questions are raised whether Iran is still isolated from the rest of … …American Journal of Applied Sciences, Vol. 10, Issue 12 · 10 Oct 2013 · 6,854 accessesView PDFCite
Research ArticleOpen accessIn the abstractPolymerase Chain Reaction Detection of Pasteurella multocida Type B: 2 in Mice Infected with Contaminated River WaterFaez Firdaus Jesse Abdullah, Mohammed Muqdad Khaleel, Lawan Adamu et al.Hemorrhagic septicemia is an acute, deadly disease of cattle and buffaloes associated with colossal economic loss in the livestock industry in the Asian regions particularly Malaysia. Therefore, this study was conducted to investigate on the Polymerase chain reaction detection of Pasteurella multocida type B: 2 in mice inoculated through different routes with river water contaminated with infected mice carcasses. Sixty … …American Journal of Animal and Veterinary Sciences, Vol. 8, Issue 3 · 17 Sep 2013 · 7,396 accessesView PDFCite
Research ArticleOpen accessIn the abstractAnticancer Activity of Isolated Chemical Constituents from Miliusa smithiaeChonthicha Naphong, Wilart Pompimon, Punchavee SombutsiriMiliusa plants belonging to the family Annonaceae are found in Thailand and have been used as Thai traditional medicines. There have been a few previously reports on the chemical constituents of plants in this genus, describing the presence of aporphine alkaloids, terpenoids, flavonoids, phenylpropanoids, styrylpyrones, bis-styryls and homogentisic acid derivatives. Miliusa smithiae, a new species for Thailand and world, has … …American Journal of Applied Sciences, Vol. 10, Issue 8 · 24 Jul 2013 · 7,263 accessesView PDFCite
Research ArticleOpen accessIn the abstractRapid Real Time PCR Based Detection of Cell Count in Case of Urinary Tract InfectionPoulomi Nandy, Ashoke Ranjan Thakur, Shaon Ray ChaudhuriMicrobial identification and antimicrobial susceptibility testing methods currently used in clinical microbiology laboratories require at least two to three days because they rely on the growth and isolation of micro-organisms. This long, but necessary, delay has enormous consequences on prophylactic usage of antimicrobial drugs. This study was an attempt to reduce this detection time span. Taq Man Real Time PCR … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 2 · 22 Jun 2013 · 8,217 accessesView PDFCite
Research ArticleOpen accessIn the abstractStaphylococcus aureus Nasal Carriage Among Injecting and Non-Injecting Drug Users and Antimicrobial SusceptibilityMojtaba Varshochi, Zahra Attari, Alka Hasani, Ali SeidiStaphylococcus Aureus (SA) is one of the most prevalent bacterial pathogens in human beings. Approximately 20% of healthy persons are persistent carriers and 60% are intermittent carriers of SA. Nasal cavity is one of the most important sites of its colonization. Intravenous (IV) drug abuse has been proposed as a risk factor for colonization of SA in the nasal mucosa. … …American Journal of Infectious Diseases, Vol. 9, Issue 2 · 23 May 2013 · 7,315 accessesView PDFCite