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140 articles for “Amino Acid”
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Research ArticleOpen accessIn the abstractTRANSCRIPTION ACTIVATOR-LIKE EFFECTOR NUCLEASES-AMAZING TOOL FOR GENOME EDITINGAvantika Singh, Vivek Pratap SinghTranscription Activator-Like Effector Nucleases (TALENs) are used to cut specific target DNA sequence in order to knock out a gene or to change its sequence. They are formed by the fusion of TALE protein serving as DNA binding domain along with non-specific DNA cleaving nucleases. Its specificity towards DNA bases in the target sequence is derived from the structure of … …OnLine Journal of Biological Sciences, Vol. 13, Issue 3 · 4 Sep 2013 · 8,000 accessesView PDFCite
Research ArticleOpen accessIn the abstractRendezvous with Tat: Transactivator of Transcription during Human Immunodeficiency Virus PathogenesisSneham Tiwari, Madhavan P.N. Nair, Shailendra K. SaxenaAcquired immune deficiency syndrome is a deadly disease caused by HIV, declared as a global catastrophe engulfing millions of lives per year. It took ~1.8 million lives in the year 2010 alone, as per reports of UNAIDS. HIV-1 is an enveloped retrovirus, having genome composed of two copies of ssRNA. It attacks the T-lymphocytes of the hosts, causes several multifaceted … …American Journal of Infectious Diseases, Vol. 8, Issue 2 · 31 Jul 2012 · 7,627 accessesView PDFCite
Research ArticleOpen accessIn the abstractPapain, a Plant Enzyme of Biological Importance: A ReviewFlorence Mamboya, Ezekiel AmriPapain is a plant proteolytic enzyme for the cysteine proteinase family cysteine protease enzyme in which enormous progress has been made to understand its functions. Papain is found naturally in papaya (Carica papaya L.) manufactured from the latex of raw papaya fruits. The enzyme is able to break down organic molecules made of amino acids, known as polypeptides and thus … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 2 · 22 Jun 2012 · 44,738 accessesView PDFCite
Research ArticleOpen accessIn the abstractCloning of the Endoglucanase Gene from a Bacillus amyloliquefaciens PSM 3.1 in Escherichia coli Revealed Catalytic Triad Residues Thr-His-GluZeily Nurachman, Sari Dewi Kurniasih, Ferra Puspitawati et al.Problem statement: An Indonesian marine bacterial isolate, Bacillus amyloliquefaciens PSM 3.1 was isolated for hydrolyzing cellulose. A 1500-bp nucleotide fragment was amplified from the chromosomal DNA by the use of primers directed against the conserved sequence of Bacilli endoglucanase genes obtained from GenBank. Approach: The fragment was cloned and expressed in Escherichia coli. Results: The endoglucanase gene (eglII gene) had … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 9,727 accessesView PDFCite
Research ArticleOpen accessIn the abstractToxicological Response of the Green Alga Chlorella vulgaris, to Some Heavy MetalsE. Afkar, H. Ababna, A. A. FathiProblems statement: The disturbance of aquatic ecosystems provoked by heavy metals pollution from industrial and domestic sources, has as consequence the loss of biological diversity, as well as increased bioaccumulation and magnification of toxicants in the food chain. The aim of this study was to evaluate the effect of some heavy metals on some physiological activities of Chlorella vulgaris beyerinck … …American Journal of Environmental Sciences, Vol. 6, Issue 3 · 30 Jun 2010 · 10,787 accessesView PDFCite
Research ArticleOpen accessIn the abstractMetabolic Fingerprinting of Three Malaysian Ginger (Zingiber officinale Roscoe) Using Gas Chromatography-Mass SpectromertryH.J. Mahdi, R. Andayani, IshakProblem statement: There is a chemical variation among different ginger cultivars detected by previous studies including the volatile and non-volatile oleo-resins. In this study we try to determine whether these variations are related to a genetic factor rather than environmental or intrinsic factors. Approach: A comprehensive metabolic fingerprinting from the leaves of three micro-propagated ginger cultivars Bukit Tinggi, Tanjung Sepat … …American Journal of Applied Sciences, Vol. 7, Issue 1 · 31 Jan 2010 · 7,390 accessesView PDFCite
Research ArticleOpen accessIn the abstractHydrophobic, Polar and Hydrogen Bonding Based Drug-Receptor Interaction of TetrahydroimidazobenzodiazepinonesV.K. Sahu, A.K.R. Khan, R.K. Singh, P.P. SinghAnti-HIV drug discovery has been increasingly focusing on HIV-1-RT (reverse transcriptase) as a potential therapeutic target. Tetrahydroimidazobenzodiazepinone (TIBO) belongs to non-nucleoside group of reverse transcriptase inhibitors (NNRTIs). A computational chemistry study has been performed on a series of tetrahydroimidazo-benzodiazepinones as HIV-1-NNRT inhibitors. Problem statement: In order to search out new drug of desired activity from the lead compounds, there was … …American Journal of Immunology, Vol. 4, Issue 3 · 30 Sep 2008 · 8,839 accessesView PDFCite
Research ArticleOpen accessIn the abstractC-Terminal Region of Dengue Virus Nonstructural Protein 1 Is Involved in Endothelial Cell Cross-Reactivity via Molecular MimicryShu-Wen Wan, Chiou-Feng Lin, Mei-Chun Chen et al.Infection with dengue virus (DV) causes diseases ranging from self-limited dengue fever to life-threatening dengue hemorrhagic fever and dengue shock syndrome. Vascular leakage, thrombocytopenia and bleeding are the clinical manifestations associated with dengue hemorrhage. We previously showed that anti-DV nonstructural protein 1 (NS1) antibodies (Abs) cross-reacted with endothelial cells. The potential target proteins on endothelial cell surface recognized by anti-DV … …American Journal of Infectious Diseases, Vol. 4, Issue 1 · 31 Mar 2008 · 7,564 accessesView PDFCite
Research ArticleOpen accessIn the abstractPrediction of a Putative Functional Region in the Human Bax Protein by Computational AnalysisMaría Sánchez-Aguilar, Laurence A. Marchat, Absalom ZamoranoStructural domains are relevant elements for interactions between Bcl-2 family members to regulate apoptosis and cell survival. Although BH1-BH4 domains can be identified by their sequence and selective occurrence in Bcl-2-family members, structural regions have not been entirely determined yet. The functional residues of Bax, the most representative pro-apoptotic protein of the human Bcl-2 family, remain almost unknown. Here, we … …American Journal of Infectious Diseases, Vol. 3, Issue 2 · 30 Jun 2007 · 7,235 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Characterization of Soybean Mosaic Virus NIa Protein and its Processing Event in Bacterial ExpressionBong K. Choi, Jae S. Park, Hye J. Ahn et al.Soybean mosaic virus (SMV)-CN18 is an Rsv resistance-breaking (RB) isolate to overcome soybean resistance genes Rsv1, Rsv3 and Rsv4. The aim of this study was to characterize nuclear inclusion protein a (NIa protein) of RB isolate at the molecular level and demonstrate its processing into genome-linked protein (VPg) and NIa-Pro domains in Esherichia coli containing a bacterial expression pET vector … …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 4 · 31 Dec 2006 · 6,906 accessesView PDFCite