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147 articles for “Chromatography”
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Research ArticleOpen accessIn the abstractRole of Evaporation in Degrading the "Oil Lakes"Al Rashed Ahamad, Colin BarkerProblem statement: Oil Spills are degraded by aerobic bacteria, water washing, evaporation and oxidation. In the absence of water the first two of these processes cannot operate. This is the situation in the low rainfall environment of the Kuwaiti desert where oil lakes still persist 20 years after the initial spills. The oils contain longer chain n-alkanes but have lost … …American Journal of Environmental Sciences, Vol. 7, Issue 3 · 11 Jul 2011 · 6,750 accessesView PDFCite
Research ArticleOpen accessIn the abstractBioassay Guided Isolation of Antibacterial Compounds from Andrographis paniculataAbubakar Sule, Qamar Uddin Ahmed, Othman Abd. Samah et al.Problem statement: Chronic disease-causing bacteria of medical importance have developed resistance to antibiotics, hence, necessitating distinct and constant need for safe and efficient therapeutic agents. Plants are considered potent candidate for this aim. A way out of reducing antibiotic resistance and adverse effects on host is the employment of antibiotic resistance inhibitors of plant origin. Approach: About 5 kg pulverized … …American Journal of Applied Sciences, Vol. 8, Issue 6 · 13 Jun 2011 · 10,027 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Phytochemical Contents and Antimicrobial Activities of Malaysian Calophyllum RubiginosumSuhaib Ibrahim ALkhamaiseh, Muhammad Taher, Farediah AhmadProblem statement: Many species of plants in Malaysia are widely used in folk medicine. However, Calophyllum species have been used in traditional medicine for their therapeutic values for many years. Several studies reported that antimicrobial, antifungal, anti-HIV and anti-cancer compounds were isolated from numerous Calophyllum species. Approach: The stem bark was extracted by EtOH, after with it was fractionated with … …American Journal of Applied Sciences, Vol. 8, Issue 3 · 25 Mar 2011 · 7,579 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Plant Growth Regulators on Callus, Cell Suspension and Cell Line Selection for Flavonoid Production from Pegaga (centella asiatica L. urban)Suat Hian Tan, Radzali Musa, Arbakariya Ariff, Mahmood MaziahProblem statement: Considering pegaga medicinal properties and over-exploitation, the requirement for a tissue culture technique as an alternative production system was crucial. Approach: Investigation of cell suspension culture response to different plant growth regulators (PRGs) for flavonoid production from elite cell line was carried out. Callus cultures were initiated from the leaf explants of Centella asiatica on Murashige and Skoog … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 11,261 accessesView PDFCite
Research ArticleOpen accessIn the abstractExtraction and Purification of Collagenase Enzymes: A Critical ReviewSaid M Daboor, Suzanne M Budge, Abdel E Ghaly et al.Problem statement: Enzymes have vital roles in several industrial processes (foods, cosmetics, nutraceuticals and pharmaceuticals) due to their highly selective nature and high activity at very low concentrations. Recent efforts to identify new sources of useful enzymes have been concentrated on the marine environment because of the potential to make use of processing wastes. About 35-50% of the mass of … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 14,320 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffects of Metabolite Combinations Produced by Lactobacillus plantarum on Plasma Cholesterol and Fatty Acids in PigletsT. V. Thu, Loh Teck Chwen, H. L. Foo et al.Problem statement: Hypercholesterol and fatty acids in plasma are the main causes for cardiovascular disease. Reduction of risk factors from diet that associated with cardiovascular disease has much attention in animals as well as in human. The objective of this study was to investigate the effects of feeding liquid metabolite combinations produced by five L. plantarum strains on the fatty … …American Journal of Animal and Veterinary Sciences, Vol. 5, Issue 4 · 27 Dec 2010 · 7,635 accessesView PDFCite
Research ArticleOpen accessIn the abstractCharacteristics of a Novel Highly Thermostable and Extremely Thermophilic Alkalitolerant Amylase from Hyperthermophilic Bacillus Strain HUTBS71Farouk Al-Quadan, Hazem Akel, Rasha NatshiProblem statement: This study reported the purification and characterization of a novel highly thermostable alkaline amylase from a newly isolated Bacillus strain HUTBS71. Approach: The enzyme was purified using ammonium sulfate precipitation, ion exchange and gel filtration chromatography. Results: Maximum amylase activity (72 U mL-1) was obtained at 100°C after 10 min of incubation. The enzyme was purified 24 fold … …OnLine Journal of Biological Sciences, Vol. 9, Issue 3 · 30 Sep 2009 · 7,404 accessesView PDFCite
Research ArticleOpen accessIn the abstractPurification of Chymotrypsin-Trypsin Inhibitor from Winged Bean Seeds using Single Step Immunoaffinity ColumnSanhita Roy, Samir Kumar DuttaProblem statement: Winged bean is a rich source of protease inhibitors. The seeds of winged bean contain several serine protease inhibitors of which winged bean chymotrypsin-trypsin inhibitor (WbCTI) is of significant importance due to its dual inhibition of both chymotrypsin and trypsin. The purification of WbCTI has always been difficult and a time taking process that involved large number of … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 3 · 30 Sep 2009 · 7,099 accessesView PDFCite
Research ArticleOpen accessIn the abstractCross Reactivity between Dromedary Whey Proteins and IgG Anti Bovine α-Lactalbumin and Anti Bovine β-LactoglobulinN. Youcef, D. Saidi, F. Mezemaze et al.Problem statement: Our aim was to enhance the data on antigenic properties of dromedary whey proteins. Approach: The identification of the whey proteins was carried by SDS-page and Reversed phase high performance liquid chromatography (RP-HPLC). The cross-reactivity of dromedary whey proteins with IgG anti bovine β-lactoglobulin and anti bovine α-lactalbumin, obtained by immunisation of Balb/c mice, was carried out by … …American Journal of Applied Sciences, Vol. 6, Issue 8 · 31 Aug 2009 · 7,432 accessesView PDFCite
Research ArticleOpen accessIn the abstractAerobic Biodegradation of Per-Treated Methyl Tert-Butyl Ether By Ozonation in an Up-Flow-Fixed-Bed ReactorM. Sadeghi, M. Arbabi, A. Nikpey, G. MardaniProblem Statement: MTBE is a common pollution of environmental and has become an issue of considerable concern in recent years. It is not readily amenable to remove MTBE by conventional techniques in water treatment. In the present study, the feasibility of the continuous aerobic biodegradation of MTBE, was evaluated in an Up- Flow Fixed Bed Reactor (UFBR). Approach: The UFBR … …American Journal of Environmental Sciences, Vol. 5, Issue 3 · 30 Jun 2009 · 6,623 accessesView PDFCite