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1,248 articles for “T-cell”
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Research ArticleOpen accessIn the abstractLarge Scale Production and Characterization of Anti-Human IgG Monoclonal Antibody in Peritoneum of Balb/c MICEB. Baradaran, J. Majidi, Z. M. Hassan, J. AbdolalizadehMonoclonal antibodies are key reagents that are used in biomedical researches, diagnosis of immunodeficiency diseases such as IgG subclasses deficiency and treatment of diseases like infections and cancers. For large scale production of monoclonal antibody, hybridoma cells that produce monoclonal antibody against human IgG were injected into the peritoneum of the Balb/c mice which have previously been primed with 0.5 … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 4 · 31 Dec 2005 · 7,089 accessesView PDFCite
Research ArticleOpen accessIn the abstractSoluble Tumor Necrosis Factor Receptor Type I and Dranulysin as Immunological Markers in Congestive Heart FailureSimone de Lima e Silva Deo, Maria Do Carmo Valente De Crasto, Alda Maria Da-Cruz et al.Previous studies have shown that circulating levels of cytokines are increased in patients with congestive heart failure (CHF), resulting in myocardial depression. In this work, we have determined serum levels of TNF-α and IFN-γ by ELISA, detected sTNFR-I by dot ELISA, as well as TNFα, IL-6, IL-10 and granulysin mRNA in unstimulated peripheral blood mononuclear cells (PBMC) by RT-PCR in … …American Journal of Immunology, Vol. 1, Issue 3 · 30 Sep 2005 · 6,773 accessesView PDFCite
Research ArticleOpen accessIn the abstractTowards Identifying Immunogenic Targets in Visceral Leishmaniasis: Role of 17kDa and 63kDa PhosphoproteinsShubhankar K Singh, Sanjiva Bimal, Diwakar S. Dinesh et al.We observed predominant phosphorylation at Protein Kinase C (PKC-α) residue in seven immunoreactive proteins of virulent Leishmania donovani parasites. These phosphoproteins were tested for their ability to stimulate T-cells for secretion of Macrophage Migration Inhibition Factor (MIF) in comparision to crude soluble antigen in Visceral Leishmaniasis (VL) patients. Two of these phosphorylated L. donovani antigens led Leishmania to present these … …American Journal of Immunology, Vol. 1, Issue 3 · 30 Sep 2005 · 6,756 accessesView PDFCite
Research ArticleOpen accessIn the abstractHumoral Immune Response in Tuberculous PleuritisPrabha C., Kripa V. Jalapathy, Sulochana D. DasTuberculous pleuritis is a good human model to understand the local and protective immune response against tuberculosis, due to the self-limitedness of the disease. Although the cellular immune response has been well characterised in tuberculous pleurisy, much less is known about the humoral immune response operating at the site of infection. To understand the humoral immune response, B cells were … …American Journal of Immunology, Vol. 1, Issue 2 · 30 Jun 2005 · 6,518 accessesView PDFCite
Research ArticleOpen accessIn the abstractExpression, Purification and Activity Assay of Two New Recombinant Antagonists of Fibrinogen ReceptorJianbo Yang, Jia Yao, Kun Yang et al.The gene sequence of Decorsin which is extracted from a kind of North American leeches was synthesized. Two recombinant proteins, Annexin V plus Decorsin (AnnV-D39) and Annexin V plus the carboxyl terminal 27 amino acid residues of Decorsin(AnnV-D27), were constructed. And a 10 amino acids linker peptide of GGGGSGGGGS was inserted between Annexin V and Decorsin in AnnV-D39. Using pET-28(a+) … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 2 · 30 Jun 2005 · 6,913 accessesView PDFCite
Research ArticleOpen accessIn the abstractImplementation of a Novel Concatenated FEC by RS and Irregular Turbo Codes on OFDM SystemsA. Shanmugam, A. R. Abdul Rajak, S. Vishwanath, R. UpendraCode Division Multiple Access (CDMA) performs well in a multi-cellular environment where a single frequency is used in all cells. Code Division Multiple Access (CDMA) has severe problems associated with multipath and Channel news. One possible problem is that the receiver may require a very large dynamic range in order to handle the large signal strength variation among users. Due … …American Journal of Applied Sciences, Vol. 2, Issue 5 · 31 May 2005 · 7,039 accessesView PDFCite
Research ArticleOpen accessIn the abstractImmunofluorometrical Exploring of Cortisol Hormone Levels in the Serum of Patients with Histologically Verified Microcellular Lung CancerIvan MilosevicIt is already known in the science that the cells of microcellular lung cancer can produce ACTH. By following the levels of Cortisol as one consequence of ACTH producing, in the serum of microcellular lung cancer patients, we could make some statistical conclusions how significant these levels would be in eventual future early diagnostic procedures, besides already existing tumormarkers etc. … …American Journal of Immunology, Vol. 1, Issue 1 · 31 Mar 2005 · 7,177 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Development of a New, Rapid, Amperometric Immunosensor for the Detection of Low Concentrations of Bacteria Part II: Optimization of the System for Escherichia coliEric Carnes, Ebtisam WilkinsThe optimization of a flow-through immunoassay system was conducted to achieve rapid detection of low concentrations of E. coli bacteria. The system includes a new immunosensor that uses newly developed, disposable immunofiltration membranes to yield a system that is sensitive as well as simple. Comparison of the performance of this system with conventional immunoassays demonstrated a significantly shorter time for … …American Journal of Applied Sciences, Vol. 2, Issue 3 · 31 Mar 2005 · 6,780 accessesView PDFCite