113 articles for “protein expression”

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Research ArticleOpen accessAll words, across the recordCryptolepine Provokes Changes in the Expression of Cell Cycle Proteins in Growing CellsCharles Ansah, Nigel J. GooderhamProblem statement: Cryptolepine, the antimalarial plant alkaloid is cytotoxic to mammalian cells, but there is rather little understanding of the possible mechanisms involved in the cell-kill action of cryptolepine. Approach: We examined mammalian cells exposed to cryptolepine (0.5-5.0 μM) for effects on the cell cycle and associated alterations in the levels of major regulatory proteins using immunoblotting and flow cytometry. … …American Journal of Pharmacology and Toxicology, Vol. 4, Issue 4 · 31 Dec 2009 · 7,396 accessesView PDF Cite

Research ArticleOpen accessAll words, across the recordHeterologous Expression and Characterization of Thermostable Levansucrase (BsSacB) from Bacillus subtilis BB03Viniti Vaidya, Gajjeraman Prabu, D. Theertha PrasadThermostable levansucrase encoding gene (sacB) with its promoter was isolated from Bacillus subtilis BB03. BsSacB is composed of 1419 bp, encoding 473 amino acid residues and with promoter regions TTGCAA (-35) and TAGAAT (-10). The amino acid sequence analysis of BsSacB showed conserved motifs of microbial fructosyltransferase belonging to Glycoside Hydrolase family 68 (GH68). Comparative analysis of the protein structure … …OnLine Journal of Biological Sciences, Vol. 15, Issue 1 · 25 Mar 2015 · 7,371 accessesView PDF Cite

Research ArticleOpen accessAll words, across the recordDetection and Localization of Anti and Pro-apoptotic mRNA Genes in Human Colorectal Cancer Using in situ RT-PCRMohd. Nazil Salleh, Boo Siaw Shi, Wan Khairuzzaman Wan Ramli et al.Problem statement: Recent studies using conventional Polymerase Chain Reaction have shown that anti-apoptotic (Cyclooxygenase-2, COX-2 and Nuclear Factor-κB, NF-κB) and proapoptotic mRNA (Bax and Bad) are involved in the tumorigenesis of colorectal cancer. The aim of this study was to localize the expression of anti and pro-apoptotic mRNA genes using Reverse Transcription in situ Polymerase Chain Reaction (RT in situ … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 3 · 30 Sep 2010 · 7,441 accessesView PDF Cite