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161 articles for “prime”
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Research ArticleOpen accessIn the abstractSerological and Molecular Investigation of Cutaneous Leishmaniasis in Healthy Individuals from an American Cutaneous Leishmaniasis-Endemic RegionRejane Cristina Ribas-Silva, Taisa Rocha Navasconi, Laís de Souza Braga et al.American Cutaneous Leishmaniasis (ACL) is a major, clinically relevant zoonosis in Brazil. The disease spectrum includes single, localized, cutaneous ulcers, diffuse cutaneous leishmaniasis and mucosal disease. A subclinical form of the disease has also been described in individuals living in ACL-endemic regions. The goal of this study was to employ immunological and molecular diagnostic methods to evaluate the presence of … …American Journal of Infectious Diseases, Vol. 11, Issue 1 · 23 Jun 2015 · 7,328 accessesView PDFCite
Research ArticleOpen accessIn the abstractFungal Concentrations in Dust Samples of Animal Rearing Houses of Bangalore, IndiaPavan RangahanumaiahFungal concentrations in dust samples analyzed by molecular methods were carried out of animal rearing house at Hessaraghatta village, Bangalore. Handy air sampler EnviroTech-APM 821 was used to collect the dust samples in triplicate and sampling time was limited to eight hours. The sampled filter papers were used for the assay of DNA extracted from all the four animal rearing … …Current Research in Microbiology, Vol. 6, Issue 1 · 8 Jun 2015 · 7,537 accessesView PDFCite
Research ArticleOpen accessIn the abstractClosing the Gaps in Rat Cytomegalovirus ALL-03 (Malaysian Strain) Genomic ScaffoldKrishnan Nair Balakrishnan, Ashwaq Ahmed Abdullah, Yusuf Abba et al.Next generation sequencing technologies has revolutionized genomic research by producing a large volume of sequence data and lowest per base cost compared to the traditional sanger method. Although this technology offers many advantages, gap occurrences are commonly found in draft assemblies. The same problem was observed with Rat Cytomegalovirus (RCMV) ALL-03 (Malaysia strain), where a complete genome sequence could not … …American Journal of Animal and Veterinary Sciences, Vol. 10, Issue 3 · 8 Jun 2015 · 7,581 accessesView PDFCite
Research ArticleOpen accessIn the abstractEvaluation of Different Drugs in Down-Regulation of Efflux Pump Genes expression in Methicillin-Resistant Staphylococcus aureus StrainsManu Chaudhary, Santosh Kumar Patnaik, Anurag PayasiAntimicrobial resistance caused by efflux systems is not widely studied in Staphylococcus aureus. In the present study, prevalence of NorA and NorB efflux pumps was studied in thirteen Methicillin-Resistant Staphylococcus aureus (MRSA) strains using a pair of primers with Polymerase Chain Reaction (PCR). Antibiotic susceptibility study in NorA and NorB efflux positive isolates was carried out according to the Clinical … …American Journal of Infectious Diseases, Vol. 10, Issue 4 · 7 Jan 2015 · 7,454 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Thyme, Clove and Cinnamon Essential Oils on Candida Albicans and Moulds Isolated from Different SourcesI.A. Radwan, A.H. Abed, M.R. Abeer et al.In this study, mycological examination were conducted on different samples from human, animals and poultry in El-Fayoum and Beni-Suef governorates, Egypt in the period from January to June 2013. The antifungal activities of thyme, clove and cinnamon oils against the recovered fungal isolates were tested using agar dilution method. A total of 209 samples were collected (including vaginal swabs from … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 4 · 26 Dec 2014 · 10,503 accessesView PDFCite
Research ArticleOpen accessIn the abstractH-RABIN CRYPTOSYSTEMHayder Raheem HashimCryptography is the science of using mathematics that's used to hide information or data that is being sent between participants in a way that prevents other people from reading it. The need of exchanging messages secretly promoted the creation of cryptosystems to enable receivers to interpret the exchanged information. In this study, a particular public key cryptosystem called Rabin Cryptosystem … …Journal of Mathematics and Statistics, Vol. 10, Issue 3 · 10 May 2014 · 7,644 accessesView PDFCite
Research ArticleOpen accessIn the abstractASSESSING THE GENETIC RELATIONSHIP OF TAIF ROSE WITH SOME ROSE GENOTYPES (ROSA SP.) BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA, INTER SIMPLE SEQUENCE REPEAT AND SIMPLE SEQUENCE REPEAT MARKERSSalah El-Din El-Assal, Mohamed A. El-Awady, Adel El-Tarras, Gaber ShehabTaif-roses are a famous rose type that cultivated in Taif region and well known with their deep and intensive fragrance in the Arabian World. Despite of the great economical importance of Taif-roses for the kingdom of Saudi Arabia, their genetic origin has not been yet elucidated. The present study was mainly aimed to assess the genetic relationship between Taif-roses and … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 29 Apr 2014 · 7,815 accessesView PDFCite
Research ArticleOpen accessIn the abstractGENETIC DIVERSITY OF OKRA (ABELMOSCHUS ESCULENTUS L.) GENOTYPES FROM DIFFERENT AGRO-ECOLOGICAL REGIONS REVEALED BY AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSISNaser M. SalamehThis study was carried out to assess the genetic diversity in 48 genotypes and accessions using eight Amplified Fragment Length Polymorphism (AFLP) primer-pairs. The eight selected AFLP primer-pairs generated a total of 150 polymorphic loci. Using the generated AFLP data, the Un-Weighted Pair Group Method with Arithmetic Average (UPGMA) ordered the genotypes into six groups based on Dice similarity coefficient. … …American Journal of Applied Sciences, Vol. 11, Issue 7 · 29 Apr 2014 · 8,078 accessesView PDFCite
Research ArticleOpen accessIn the abstractCOMPARATIVE ANALYSIS OF INTER SIMPLE SEQUENCE REPEATS AND SIMPLE SEQUENCE REPEATS MARKERS: GENETIC ANALYSIS OF DESCHAMPSIA CESPITOSA POPULATIONS GROWING IN METAL CONTAMINATED REGIONS IN CANADAK.K. Nkongolo, S. Gervais, P. Michael, Y. ZhouComparative studies conducted on the genetic variation of metal-tolerant populations and their non-metal-tolerant counterparts have been performed on numerous species using isozyme markers. Analysis of genetic differences among plant populations growing in heavy metal-contaminated and uncontaminated regions are limited. The main objectives of the present study were to compare ISSR and microsatellite markers in assessing genetic variation in D. cespitosa … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 8 Apr 2014 · 7,291 accessesView PDFCite
Research ArticleOpen accessIn the abstractRANDOMLY AMPLIFIED POLYMORPHIC DNA-A BRIEF REVIEWNandani Kumari, Saroj Kumar ThakurRAPD is a PCR based technique which involves the use of single arbitrary short primers (8-12 nucleotides), resulting in the amplification of many discrete DNA. The segments of DNA that are amplified are random. The technique was developed independently by two different laboratories and called as RAPD and AP-PCR (Arbitrary Primed PCR). This procedure detects nucleotide sequence polymorphisms in a … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 1 · 2 Jan 2014 · 17,266 accessesView PDFCite