117 articles for “antibodies”

Set a search alert

Research ArticleOpen accessIn the abstractLarge Scale Production and Characterization of Anti-Human IgG Monoclonal Antibody in Peritoneum of Balb/c MICEB. Baradaran, J. Majidi, Z. M. Hassan, J. AbdolalizadehMonoclonal antibodies are key reagents that are used in biomedical researches, diagnosis of immunodeficiency diseases such as IgG subclasses deficiency and treatment of diseases like infections and cancers. For large scale production of monoclonal antibody, hybridoma cells that produce monoclonal antibody against human IgG were injected into the peritoneum of the Balb/c mice which have previously been primed with 0.5 … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 4 · 31 Dec 2005 · 7,089 accessesView PDF Cite

Research ArticleOpen accessIn the abstractHuman Latrophilin-2 is Expressed in the Cytotrophoblast and Syncytiotrophoblast of Placenta and in Endothelial CellsGunda Herberth, Anke Stein, Jens Glienke et al.Latrophilin-2 is a member of the family of adhesion-GPCRs that is characterised by a long N-terminus which contains motifs identified in proteins involved in cell adhesion. We were interested in determining the expression pattern of human latrophilin-2 and to perform a biochemical characterisation of this protein. The expression pattern of latrophilin-2 was analysed in human organs, tissues and cell lines. … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 3 · 30 Sep 2005 · 8,053 accessesView PDF Cite

Research ArticleOpen accessIn the abstractHumoral Immune Response in Tuberculous PleuritisPrabha C., Kripa V. Jalapathy, Sulochana D. DasTuberculous pleuritis is a good human model to understand the local and protective immune response against tuberculosis, due to the self-limitedness of the disease. Although the cellular immune response has been well characterised in tuberculous pleurisy, much less is known about the humoral immune response operating at the site of infection. To understand the humoral immune response, B cells were … …American Journal of Immunology, Vol. 1, Issue 2 · 30 Jun 2005 · 6,518 accessesView PDF Cite

Research ArticleOpen accessIn the abstractInteraction of a C-terminal Truncated Hepatitis C Virus Core Protein with Plasmid DNA Vaccine Leads to in vitro Assembly of Heterogeneous Virus-like ParticlesNelson Acosta-Rivero, Joanna Poutou, Alexis Mussachio et al.Recently, it has been shown that HCV core proteins (HCcAg) with C-terminal deletions assemble in vitro into virus-like particles (VLPs) in the presence of structured RNA molecules. Results presented in this work showed that a truncated HCcAg variant covering the first 120 aa (HCcAg.120) with a 32 aa N-terminal fusion peptide (6xHistag-XpressTMepitope) interacts with plasmid DNA vaccine. Interestingly, the buoyant … …American Journal of Infectious Diseases, Vol. 1, Issue 1 · 31 Mar 2005 · 6,754 accessesView PDF Cite

Research ArticleOpen accessIn the abstractEvidences of Apoptosis in Hepatitis C Virus-Infected Hepatocytes and Peripheral Blood Monocuclear Cells in the Absence of Liver InjuryViviana Falcón, Nelson Acosta-Rivero, Mineko Shibayama et al.Understanding the mechanism of Hepatitis C Virus (HCV) pathogenesis is an important part of HCV research. In this study, the presence of apoptosis in HCV-infected liver and Peripheral Blood Mononuclear Cells (PBMC) from patients positive for anti-HCV antibodies and negative for serum HCV-RNA was investigated. The samples obtained from 21 patients were studied by in situ Hybridization (ISH), Immunofluorescence, TUNEL … …American Journal of Infectious Diseases, Vol. 1, Issue 1 · 31 Mar 2005 · 6,975 accessesView PDF Cite

Research ArticleOpen accessIn the abstractEvidences of Hepatitis C Virus Replication in Hepatocytes and Peripheral Blood Monocuclear Cells from Patients Negative for Viral RNA in SerumViviana Falcón, Nelson Acosta-Rivero, Mineko Shibayama et al.In this study, we examined the expression of hepatitis C virus (HCV) components in hepatocytes and peripheral blood mononuclear cells (PBMC) from patients positive for anti-HCV antibodies and negative for serum HCV-RNA. The samples obtained from 25 randomly selected patients (13 of 25 patients showed no histological evidences of chronic hepatitis and had normal serum ALAT and GGTP levels) were … …American Journal of Infectious Diseases, Vol. 1, Issue 1 · 31 Mar 2005 · 7,164 accessesView PDF Cite

Research ArticleOpen accessIn the abstractThe Development of a New, Rapid, Amperometric Immunosensor for the Detection of Low Concentrations of Bacteria Part II: Optimization of the System for Escherichia coliEric Carnes, Ebtisam WilkinsThe optimization of a flow-through immunoassay system was conducted to achieve rapid detection of low concentrations of E. coli bacteria. The system includes a new immunosensor that uses newly developed, disposable immunofiltration membranes to yield a system that is sensitive as well as simple. Comparison of the performance of this system with conventional immunoassays demonstrated a significantly shorter time for … …American Journal of Applied Sciences, Vol. 2, Issue 3 · 31 Mar 2005 · 6,780 accessesView PDF Cite