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Research ArticleOpen accessAll words, across the recordComparative Functional Analysis of the Basic Helix-Loop-Helix Proteins in the Clawed Frogs' Genomes With Common Essential Pathways and Enriched Gene Ontology TermsWuyi LiuThe international Gene Ontology (GO) and pathway databases were used to functionally analyze the clawed frogs' Basic Helix-Loop-Helix (bHLH) transcription factors of Xenopus tropicalis and Xenopus laevis in a updated genome-wide survey. There were 41 GO terms and one pathway significantly enriched for Xenopus tropicalis, whereas there were 45 GO terms and 3 pathways significantly enriched for Xenopus laevis. Among … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 1 · 28 Mar 2019 · 8,490 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordMolecular Analysis of KRAS Mutation Associated with Colorectal Cancer in Iraqi PatientsRehab Subhi Ramadhan, Aola Sabah Ali, Rebah Najah AlgafariThe role of KRAS gene was investigated in manifestation of colorectal cancer in Iraqi patients. A total 40 blood samples were collected during October 2016 to January 2017 from AL-Amal Hospital in Baghdad and 20 blood samples from healthy subjects served as control. Blood samples were collected from subjects of 40-70 years old for both patients and control. The study … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 1 · 3 Mar 2018 · 9,024 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordDiscrimination Capacity of RAPD, ISSR and SSR Markers and of their Effectiveness in Establishing Genetic Relationship and Diversity among Egyptian and Saudi Wheat CultivarsSalah El-Din El-Assal, Ahmed GaberProblem statement: Yield crop cultivars and landraces are valuable sources of genetic variations that the knowledge and implication of these variations are critical in the plant breeding programs. our major objective of this study is investigating the discriminating capacity of RAPD, ISSR and SSR markers and of their effectiveness in establishing genetic relationship and diversity among Egyptian and Saudi wheat … …American Journal of Applied Sciences, Vol. 9, Issue 5 · 9 Mar 2012 · 7,768 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordCharacterization of the Genetic Diversity of Peach Cultivars in Taif by RAPD-PCRMohamed A. Nagaty, Salah El-Din El-Assal, Mahmoud M. RifaatProblem statement: Peach genotypes from the Kingdom of Saudi Arabia were not adequately characterized at the biological and molecular levels. This study was carried out to characterize nine peach cultivars growing in Taif (KSA) at the biological and molecular levels. Approach: For the nine peach cultivars studied, flowering and fruiting indices were determined and eight, 10-base primers were used to … …American Journal of Applied Sciences, Vol. 8, Issue 7 · 24 Jun 2011 · 7,271 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordAssociation of DNA Polymorphism at the Apolipoprotein B-100 Gene Locus with Plasma Lipid Concentration and Coronary Artery Disease among North IndiansVivek Pratap Singh, V. Ramesh, Sonal Somvanshi et al.The aim of this study was to investigate the association between apolipoprotein B gene polymorphisms and coronary artery disease and lipid levels in north Indians. Two hundred patients of angiographically proven atherosclerotic CAD and two hundred age and sex matched control subjects were included in the study. Serum lipids including cholesterol, triglycerides, HDL, LDL, VLDL and ApoB were analyzed. PCR … …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 4 · 31 Dec 2006 · 7,892 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordCloning and Functional Analysis of tri14 in Trichoderma brevicompactumXu-Ping Shentu, Xiao-Feng Yuan, Wei-Ping Liu et al.Trichodermin, one of the trichothecenes, is a potential antifungal antibiotic and a protein synthesis inhibitor. Trichodermin is synthesized mainly by Trichoderma brevicompactum, but the biosynthetic pathway has yet been completely elucidated. Our earlier transcriptome assay on the trichodermin synthesis in T. brevicompactum 0248 identified a unique tri14 transcript, designated Tbtri14, that was co-regulated with other tri cluster genes. However, the … …American Journal of Biochemistry and Biotechnology, Vol. 11, Issue 3 · 25 Aug 2015 · 7,393 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordSSR Analysis of Maternal and Paternal Lines Selected in the Don Region (Russia)N. V. Markin, A. V. Usatov, V. N. Vasilenko et al.Evaluation of DNA polymorphisms of breeding material of sunflower from L.A. Zhdanov Don Experimental Station of oil Crops of V.S. Pustovoit All-Russian Research Institute of Oil Crops represented by 17 maternal (CMS) lines and 12 paternal (Rf) lines was conducted. There were identified 35 allelic variants of CMS lines and 42 allelic variants of Rf lines with use of 11 … …American Journal of Agricultural and Biological Sciences, Vol. 11, Issue 1 · 16 Jan 2016 · 7,555 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordPrevalence of miRNAs in Introns and Cis-Regulatory Regions of Genes of the Somatotropic Axis in MammalsTatiana Shkurat, Dmitry Romanov, Evgeny Pshenichnyy et al.To understand the role of microRNA in genetic control of animal growth mechanisms, we performed bioinformatic analysis of the localization of microRNAs in introns and cis-regulatory regions of the somatotropic axis genes-GH1, GHRH, SST and IGF1 in Homo sapiens, Macaca mulatta, Pan troglodytes, Pongo abelii, Gorilla gorilla, Ovis aries, Bos Taurus, Loxodonta Africana, Oryctolagus cuniculus, Canis lupus, Rattus norvegicus and … …American Journal of Applied Sciences, Vol. 12, Issue 1 · 26 Jan 2015 · 7,015 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordASSESSING THE GENETIC RELATIONSHIP OF TAIF ROSE WITH SOME ROSE GENOTYPES (ROSA SP.) BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA, INTER SIMPLE SEQUENCE REPEAT AND SIMPLE SEQUENCE REPEAT MARKERSSalah El-Din El-Assal, Mohamed A. El-Awady, Adel El-Tarras, Gaber ShehabTaif-roses are a famous rose type that cultivated in Taif region and well known with their deep and intensive fragrance in the Arabian World. Despite of the great economical importance of Taif-roses for the kingdom of Saudi Arabia, their genetic origin has not been yet elucidated. The present study was mainly aimed to assess the genetic relationship between Taif-roses and … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 29 Apr 2014 · 7,811 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordA Sequence Based Validation of Gene Expression Microarray DataGerhard G. Thallinger, Eva Obermayr, Pornpimol Charoentong et al.Problem statement: Quantitative Reverse Transcription PCR (RT-qPCR) is often used to validate microarray data. Previous studies show different levels of correlation, without further investigation of influencing factors. Approach: We compared expression levels of 381 genes obtained from microarray hybridizations and from TaqMan based RT-qPCR assays. Correlation of expression levels was determined by comparing: (i) single genes across samples, (ii) all … …Current Research in Bioinformatics, Vol. 1, Issue 1 · 19 Jan 2012 · 13,715 accessesView PDFCite