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1,059 results for “Detection”
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Research ArticleOpen accessIn the abstractHIV-1 Infection and Central Monoamine NeurotransmittersAdarsh M. Kumar, J. B. Fernandez, Irina Borodowsky et al.Human immunodeficiency virus type 1 (HIV-1) enters the central nervous system (CNS) shortly after infection and gets localized in different brain regions, leading to various types of neuropathological problems. It has been hypothesized that HIV-1 infection mediated neuropathogenesis may also adversely affect the activity of the central monoamine neurotransmitters systems, such as dopamine (DA), and 5-hydroxytryptamine (5-HT, serotonin), resulting in … …American Journal of Infectious Diseases, Vol. 3, Issue 4 · 31 Dec 2007 · 7,491 accessesView PDFCite
Research ArticleOpen accessIn the abstractInduction of the Chemokines CCL3α, CCL3α and CCL5 in Atherosclerotic PatientsAlyaa Mousa, Amani Al-Zaki, Safa Taha, Moiz BakhietChemokines recruit immune cells to inflammatory sites and promote the process of inflammation. The role of these mediators in the disease process in atherosclerosis is not fully studied. The spontaneous mRNA expression and intracellular protein production of the potential inflammatory chemokines CCL3α and CCL3β (macrophage- inflammatory protein-1α and β; CCR3α/β ligand) and CCL5 (regulated upon activation, normal T cell expressed … …American Journal of Immunology, Vol. 3, Issue 2 · 31 Dec 2007 · 6,952 accessesView PDFCite
Research ArticleOpen accessIn the abstractImmunohistochemistry Assay to Detect Turkey Coronavirus (TCoV) from Experimentally Infected PoultsThais Larissa L Castanheira, Heitor F. Ferrari, Maria Cecília R. Luvizotto, Tereza C. CardosoThe objective of this study was to develop a direct immunohistochemical assay to detect TCoV antigens in formalin-fixed paraffin-embedded sections prepared from experimentally infected poults. The sections of ileo, ileo-cecal junction and ceca regions from intestine were prepared and submitted to two different primary antibodies, first the non-biotin labeled polyclonal antibody for the indirect method, and second the biotin-labeled polyclonal … …American Journal of Immunology, Vol. 3, Issue 2 · 31 Dec 2007 · 6,900 accessesView PDFCite
Research ArticleOpen accessIn the abstractIsolation and Identification of Listeria monocytogenes in Processed Meat by a Combined Cultural-molecular MethodAngela Ingianni, Marta Quartuccio, Maria Antonietta Madeddu et al.The isolation and identification of Listeria monocytogenes in processed meat samples by a combined cultural-molecular method is described. It allows the identification of Listeria strains by means of a hybridization technique with a specific DNA probe directed to the listerial internalin gene. The specificity of this method was found to be 100% and sensitivity was as low as 1 CFU/2.5 … …American Journal of Infectious Diseases, Vol. 3, Issue 3 · 30 Sep 2007 · 7,252 accessesView PDFCite
Research ArticleOpen accessIn the abstractPrevalence of Herpesvirus 8 Infection in Type 2 Diabetes Mellitus PatientsA. Ingianni, F. Carta, A. Reina et al.In this study a possible relationship between the presence of type 2 diabetes (DM2) and the Human Herpesvirus 8 infection (HHV-8) has been evaluated. The presence of HHV-8 DNA was monitored by nested PCR and Southern blotting in peripheral blood leucocytes of DM2 patients admitted to the Diabetology and Metabolic Disease Service of the "S. Giovanni di Dio" Hospital (Cagliari, … …American Journal of Infectious Diseases, Vol. 3, Issue 3 · 30 Sep 2007 · 7,923 accessesView PDFCite
Research ArticleOpen accessIn the abstractBio-Safety Technology in Production of Bovine Herpesvirus Type 5 (BoHV-5) Using an Alternative Serum-Free MediumTereza C. Cardoso, Heitor F. Ferrari, Maria Cecília R. Luvizotto, Clarice W. ArnsThe detection and growth of bovine herpes virus type 5 (BoHV-5) was evaluated in three different media without foetal bovine serum or animal protein by infecting three different cell lines. The OPTI-PRO, VP-SFM and RPMI 1640 media were supplemented by L-glutamine, antibiotics added by 5µg of insulin-like growth factor (IGF-I) and used to adapt CER, MDBK and CRIB cells in … …American Journal of Biochemistry and Biotechnology, Vol. 3, Issue 3 · 30 Sep 2007 · 6,991 accessesView PDFCite
Research ArticleOpen accessIn the abstractDetermination of Metoprolol and Felodipine in Binary Mixture Using Chemometric-Assisted Spectrophotometric and High-Performance Liquid Chromatographic-UV MethodsHesham Salem, Ola M. AbdallahFour methods were developed for simultaneous determination of metoprolol and felodipine without previous separation. In the first method both drugs were determined using first derivative UV spectrophotometry, with zero crossing measurement at 222 and 235 nm for metoprolol and felodipine, respectively. The second method depends on first derivative of the ratios spectra by measurements of the amplitudes at 250.1 nm … …American Journal of Applied Sciences, Vol. 4, Issue 9 · 30 Sep 2007 · 8,025 accessesView PDFCite
Research ArticleOpen accessIn the abstractComputer Security: a Survey of Methods and SystemsRoman V. Yampolskiy, Venu GovindarajuIn this work we have reviewed studies which survey all aspects of computer security including attackers and attacks, software bugs and viruses as well as different intrusion detection systems and ways to evaluate such systems. The aim was to develop a survey of security related issues which would provide adequate information and advice to newcomers to the field as well … …Journal of Computer Science, Vol. 3, Issue 7 · 31 Jul 2007 · 6,824 accessesView PDFCite
Research ArticleOpen accessIn the abstractAtomic Absorption Spectrometric Determination of Certain Fluoroquinolones in Pharmaceutical Dosage Forms and in Biological FluidsH. Salem, W. Khater, L. FadaSimple, accurate, sensitive and selective methods are described for the quantitative determination of ten fluoroquinolones (amifloxacin, ciprofloxacin hydrochloride, difloxacin hydrochloride, enoxacin, enrofloxacin, lomefloxacin hydrochloride, levofloxacin, norfloxacin, ofloxacin and pefloxacin mesylate). The methods are based on precipitation of the ion associates formed from the reaction of the cited drugs with silver nitrate, copper acetate and ferric chloride. The formation and solubility … …American Journal of Pharmacology and Toxicology, Vol. 2, Issue 2 · 30 Jun 2007 · 7,419 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Value of Serologic Tests for Diagnosis and Follow up of Patients having BrucellosisNidia E. Lucero, Sandra M. Ayala, Gabriela I. Escobar, N R. JacobThough diagnosis of human brucellosis is accurate when the causal agent is isolated, this procedure is not always successful and the most of patients are diagnosed on the basis of rising titres of antibodies in serum. The classical tests used for detection of antibodies to S-Brucella sp., include Rose Bengal (RBT), buffered plate antigen (BPAT), serum agglutination (SAT), 2-mercapto-ethanol (2MET) … …American Journal of Infectious Diseases, Vol. 3, Issue 1 · 31 Mar 2007 · 7,446 accessesView PDFCite