94 articles for “primer”

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Research ArticleOpen accessIn the abstractDetermination of the Presence of Huanglongbing in Seeds and Movement of the Pathogen in Citrus reticulataHajivand Shokrollah, Thohirah Lee Abdullah, Kamaruzaman Sijam, Siti Nor Akmar AbdullahProblem statement: Huanglongbing (HLB) also known as citrus greening disease is a fastidious phloem-inhabiting bacterium in the genus Candidatus Liberibacter. Using universal primers, the 16S ribosomal DNA sequence of three strains of the bacterium were obtained by PCR. However there is very little information on seed transmission and HLB pathogen movement to find a way for control or reduce the … …American Journal of Applied Sciences, Vol. 6, Issue 6 · 30 Jun 2009 · 8,088 accessesView PDF Cite

Research ArticleOpen accessIn the abstractGenetic Diversity of Red Chittagong Cattle Using Randomly Amplified Polymorphic DNA MarkersMohammad Masud Rana Mufti, Mst. Parvin Mostari, Gautam Kumar Deb et al.Problem statement: Red Chittagong Cattle (RCC) is a potential genetic resource in Bangladesh having dairy potentialities, high resistance to disease and survive harse environmental condition while require low input supply. This resource is now disappearing due to indiscriminate crossing with other local and exotic cattle. To conserve this resource, find out the genetic diversity of RCC is prime concern. Approach: … …American Journal of Animal and Veterinary Sciences, Vol. 4, Issue 1 · 17 Mar 2009 · 8,680 accessesView PDF Cite

Research ArticleOpen accessIn the abstractImproving a PCR-Based Method for Identification of Ralstonia solanacearum in Natural Sources of West MalaysiaReza Khakvar, Kamaruzaman Sijam, Wong Mui Yun et al.Ralstonia solanacearum, a soil-borne plant pathogen, causes lethal wilting disease of more than 200 plants worldwide. This very wide host range covers both monocots and dicots, extending from annual plants to trees and shrubs. Although generally it’s considered as a plant pathogen, R. solanacearum behaves primarily as a saprophytic bacterium able to survive for long periods of time in various … …American Journal of Agricultural and Biological Sciences, Vol. 3, Issue 2 · 30 Jun 2008 · 9,469 accessesView PDF Cite

Research ArticleOpen accessIn the abstractToll-Like Receptor (TLR) 2 and TLR4 Gene Expression in Canine HeartA. Linde, F. Blecha, T. MelgarejoToll-like receptors (TLRs) are archetypal pattern recognition receptors of immediate importance for an efficacious innate immune response. TLRs exhibit marked differential tissue activity and their levels within a discrete cell type can be highly dynamic. Of 13 known mammalian paralogues, three TLRs have been identified in the dog. Although cardiac TLR expression has been reported in other species, this study … …American Journal of Animal and Veterinary Sciences, Vol. 2, Issue 1 · 31 Mar 2007 · 6,921 accessesView PDF Cite