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130 results for “enzyme activity”
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Research ArticleOpen accessIn the abstractStudy on Salivary Glands α-amylase In Wheat Bug Eurygaster maura (Hemiptera: Scutelleridae)Mohammad Mehrabadi, Ali R. Bandaniα-amylase activity in the salivary glands of Eurygaster maura was determined by biochemical experiments. Some of adult insect was collected and their salivary glands isolated and characterized. Enzyme samples from salivary glands of adults were prepared by the method of Cohen with slight modifications. α-Amylase activity was assayed based on Bernfeld method by the dinitrosalicylic acid (DNS) procedure. The activity … …American Journal of Applied Sciences, Vol. 6, Issue 4 · 30 Apr 2009 · 7,648 accessesView PDFCite
Research ArticleOpen accessIn the abstractAssessing of α-Amylase Activity of Midgut in Wheat Bug Eurygaster mauraMohammad Mehrabadi, Ali R. BandaniTo determine of α-amylase activity in the midgut of Eurygaster maura, some of adult insect was collected and their gut isolated and characterized. Enzyme samples from midguts of adults were prepared by the method of Cohen with slight modifications. α-Amylase activity was assayed based on Bernfeld method by the dinitrosalicylic acid (DNS) procedure. The activity of α-amylase in midgut was … …American Journal of Applied Sciences, Vol. 6, Issue 3 · 31 Mar 2009 · 7,629 accessesView PDFCite
Research ArticleOpen accessIn the abstractA Putative Serine protease from Larval Midgut of Red Palm Weevil Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae): Partial Purification and Biochemical CharacterizationAhmed Al Jabr, Mahmoud Abo-El-SaadA putative serine protease was isolated and partially purified from larval midgut of red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier) using anion exchange chromatography, DE-52. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of this protease showed that the major band has a molecular weight of approximately 24 kDa corresponding to the same molecular weight of mammalian trypsin. In addition, zymography … …American Journal of Environmental Sciences, Vol. 4, Issue 6 · 31 Dec 2008 · 7,025 accessesView PDFCite
Research ArticleOpen accessIn the abstractProtective Effects of Covalent Cross-Linking on Proteolysis of Human Coproporphyrinogen Oxidase and Implications for PorphyriaAhmed W. Jafri, Jason R. Stephenson, Justin B. Morgenthaler et al.The effects of covalent cross-linkers on the enzyme, coproporphyrinogen oxidase, had been previously studied but their role in protecting the enzyme from protease cleavage has not been evaluated. Therefore, we examined how the cross-linker bis (sulfosuccinimidyl) suberate (BS3) affects the ability of trypsin to digest purified, wild type recombinant human coproporphyrinogen oxidase and selected mutants. Following incubation, the apparent molecular … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 4 · 31 Dec 2008 · 6,690 accessesView PDFCite
Research ArticleOpen accessIn the abstractCharacterization and Wash Performance Analysis of Microbial Extracellular Enzymes from East Calcutta Wetland in IndiaRamesh Malathu, Sanhita Chowdhury, Madhusmita Mishra et al.Extracellular protease from a novel bacterial isolate showing maximum similarity of 98.22% with Microbacterium luteolum was obtained from East Calcutta Wetland, India. It showed compatibility with commercial detergents. The enzyme retains more than 60% of its activity between 6.0 to 10.5 pH. The maximum activity is at pH 7.5 with 71% activity at pH 10.0 and 10.5. The protease retained … …American Journal of Applied Sciences, Vol. 5, Issue 12 · 31 Dec 2008 · 8,731 accessesView PDFCite
Research ArticleOpen accessIn the abstractA Thermostable α-Amylase Producing Natural Variant of Bacillus spp. Isolated From Soil in IranIraj Rasooli, Shakiba D. A. Astaneh, Hojjat Borna, Kamal A. BarchiniThermophilic processes appear more stable, rapid and less expensive and facilitate reactant activity and product recovery. Amylases have a quarter of the world enzyme market and thermostable a-amylases possess extensive commercial applications. Since little work has been done on strain isolation, growth and enzyme yield optimization, the level of thermophilic enzyme production remains relatively low. Therefore, large scale exploitation of … …American Journal of Agricultural and Biological Sciences, Vol. 3, Issue 3 · 30 Sep 2008 · 8,907 accessesView PDFCite
Research ArticleOpen accessIn the abstractOptimum Conditions for Measuring Dehydrogenase Activity of Aspergillus niger using TTCA. E. Ghaly, N. S. MahmoudThe suitability of the triphenyl tetrazolium chloride (TTC) for dehydrogenase activity measurement of the vegetative cells of the fungal species Aspergillus niger was investigated. The triphenyl formazan (TF) yield increased with the increases in TTC concentration, pH, temperature and incubation time within the studied ranges. The effects of individual parameters, as well as the combined effects, on the TF yield … …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 4 · 31 Dec 2006 · 9,008 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Feed Additives on the Development of Proteolytic Enzymes of the Tropical Sport Fish Malaysian Mahseer (Tor tambroides-Bleeker) FryK.C.A. Jalal, M. A. Ambak, M.A.B. Abol et al.Tor tambroides fry with a mean of 8.0±0.72 mm standard length (SL) and weighing (W) 0.06±0.01g were stocked at the rate of fifty (50) individuals in each of the fifteen 150l rectangular fibre glass tanks for a period of 5 weeks. The development of proteolytic enzymes (Trypsin and Chymotrypsin) was studied during these feeding treatments. 45% protein diet without additives … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 3 · 30 Sep 2005 · 7,002 accessesView PDFCite
Research ArticleOpen accessIn the abstractA New Method to Estimate Intrinsic Parameters in the Ping-pong Bisustrate Kinetic: Application to the Oxipolymerization of PhenolJosé L. Gómez, Antonio Bódalo, Elisa Gómez et al.A new method for determining the intrinsic parameters of reaction in processes involving a high initial rate has been developed. The usefulness of this alternative, which consists of determining several sets of apparent parameters at different times and then extrapolating these to time zero, is demonstrated proved by the linear dependence obtained between the apparent parameters and the reaction time. … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 2 · 30 Jun 2005 · 6,534 accessesView PDFCite
Research ArticleOpen accessIn the abstractPurification of A Recombinant Thrombin-like Enzyme, Gloshedobin by Egg Yolk Antibody-Coupled AdsorbentsQing Yang, Xuyu Lei, Jianqiang Xu, Lijia AnThe gloshedobin, a snake venom thrombin-like enzyme was biosynthesized in the soluble form and purified by egg yolk antibody-coupled adsorbents from E. coli. As His-tag is not favored from the point of view of high-level and soluble expression, we herein constructed a recombinant gloshedobin without His-tag and developed a novel egg yolk antibody (IgY)-immunoaffinity chromatography for its purification in a … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 1 · 31 Mar 2005 · 6,694 accessesView PDFCite