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92 results for “Incubation”
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Research ArticleOpen accessIn the abstractHydroxyapatite-induced Phagocytic Activity by Murine Macrophages in vitroV.K. Gopinath, M. Musa, A.R. Samsudin et al.The aim of the present study was to assess phagocytic activities of murine macrophages (RAW264.7 cell line) to hydroxyapatite (HA) particles. Transmission electron microscope (TEM) was used to assess localization of HA particles in the cells at 7, 15, 30 and 60 min. The HA particles and latex beads, as a control, were also incubated with the cells pretreated with … …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 3 · 30 Sep 2006 · 6,375 accessesView PDFCite
Research ArticleOpen accessIn the abstractUse of Cross-linking to Assess Subunit Interaction of Recombinant Human Coproporphyrinogen OxidaseJason R. Stephenson, Nancy E. Thomas, Jon A. Friesen, Marjorie A. JonesTo provide further evidence for a dimeric form of coproporphyrinogen oxidase reported using the conventional hydrodynamic methods, bifunctional cross-linkers were incubated with purified, recombinant human coproporphyrinogen oxidase to determine subunit interaction in solution. The use of cross-linkers provides an effective way to demonstrate subunit association and allows for assessment of activity upon covalent cross-linking. Following incubation with selected cross-linkers, enzyme … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 2 · 30 Jun 2005 · 6,597 accessesView PDFCite