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11 results for “M. A. Steele”
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Research ArticleOpen accessIn the author namesThe Effects of Subacute Ruminal Acidosis on Milk Fatty Acid Profile in Dairy CattleC. Mitchell, O. ALZahal, M.M. Or-Rashid et al.The objective of this experiment was to examine the effect of Subacute Ruminal Acidosis (SARA) on milk fatty acid (FA) profiles with emphasis on odd-chain and branched-chain FA. Sixteen multiparous Holstein dairy cows (618±35 kg of body weight, 221±32 days in milk) were used in a randomized complete-block design. During week 1, all cows received a high-forage diet (90% chopped … …American Journal of Animal and Veterinary Sciences, Vol. 11, Issue 2 · 27 Apr 2016 · 7,746 accessesView PDFCite
Research ArticleOpen accessIn the author namesChanges in the Rumen Papillae During the Periparturient Transition in Holstein Dairy Cows are Accompanied by Changes in Abundance of Proteins Involved in Intracellular pH Regulation, But not SCFA TransportAnne Hermen Laarman, Adam Kleinberg, Michael Alexander Steele et al.The objective of this study was to examine changes in SCFA transport capacity in the rumen epithelium during the parturition transition period. Twelve pregnant, cannulated primiparous and multiparous cows were fed a standard Total Mixed Ration (TMR) for the duration of the dry period; after calving, all cows were switched to an early lactation TMR. Rumen pH, fluid and biopsies … …American Journal of Animal and Veterinary Sciences, Vol. 10, Issue 1 · 31 Mar 2015 · 7,554 accessesView PDFCite
Research ArticleOpen accessIn the author namesTRANSCRIPTOMIC CHANGES IN RUMINAL TISSUE INDUCED BY THE PERIPARTURIENT TRANSITION IN DAIRY COWSLouis Dionissopoulos, Ousama AlZahal, Michael Alexander Steele et al.To understand how the capacity for fat metabolism (uptake, synthesis, modification) changes in rumen epithelia immediately before and after onset of lactation in dairy cows, rumen fluid Short Chain Fatty Acid (SCFA) concentrations and mRNA expression profiles of rumen epithelia was determined in twelve Holstein dairy cows at three weeks prior to calving (wk -3, n = 12), one week … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 1 · 13 Jan 2014 · 7,505 accessesView PDFCite
Research ArticleOpen accessIn the author namesButyrate Supplementation Affects Mrna Abundance of Genes Involved in Glycolysis, Oxidative Phosphorylation and Lipogenesis in the Rumen Epithelium of Holstein Dairy CowsAnne Hermen Laarman, Louis Dionissopoulos, Ousama AlZahal et al.Energy availability in epithelial cells is a crucial link for maintaining epithelial barrier integrity; energy depletion is linked to impaired barrier function in several epithelia. This study aimed to elucidate the effects of exogenous butyrate on mRNA abundance of genes indirectly involved in rumen epithelial barrier integrity. Sixteen mid-lactation Holstein cows fed a total mixed ration received a concentrate mix … …American Journal of Animal and Veterinary Sciences, Vol. 8, Issue 4 · 27 Nov 2013 · 7,525 accessesView PDFCite
Research ArticleOpen accessIn the author namesButyrate and Subacute Ruminal Acidosis Affect Abundance of Membrane Proteins Involved with Proton and Short Chain Fatty Acid Transport in the Rumen Epithelium of Dairy CowsAnne Hermen Laarman, Louis Dionissopoulos, Ousama AlZahal et al.The objective of this study was to elucidate the effects of butyrate on the Short Chain Fatty Acids (SCFA) membrane transport proteins and proton membrane transport proteins in the rumen epithelium. Sixteen mid-lactation cows were fed a 44% Non-Fibre Carbohydrate (NFC) diet and divided into a control treatment and a butyrate treatment. For 7 days, the cows on the control … …American Journal of Animal and Veterinary Sciences, Vol. 8, Issue 4 · 27 Nov 2013 · 7,480 accessesView PDFCite
Research ArticleOpen accessIn the author namesButyrate-Mediated Genomic Changes Involved in Non-Specific Host Defenses, Matrix Remodeling and the Immune Response in the Rumen Epithelium of Cows Afflicted with Subacute Ruminal AcidosisLouis Dionissopoulos, Anne Hermen Laarman, Ousama AlZahal et al.Subacute Ruminal Acidosis (SARA) is a disorder in cattle which can lead to chronic inflammation in the rumen epithelium, known as rumenitis. Butyrate has been shown to attenuate some of the detrimental effects of inflammatory gastroenteral disorders but the molecular mechanisms mediated by butyrate have not been defined. The objective of this study was to define the inflammatory-related genomic changes … …American Journal of Animal and Veterinary Sciences, Vol. 8, Issue 1 · 10 Jan 2013 · 8,065 accessesView PDFCite
Research ArticleOpen accessIn the author namesA Characterization of Inflammatory and Structural Markers within the Rumen Epithelium During Grain-Induced Ruminal Acidosis in Lactating Dairy CattleL. Dionissopoulos, M. A. Steele, O. AlZahal et al.The objective of this study was to characterize the mRNA and protein expression of inflammatory and structural genes in the rumen epithelium during grain-induced ruminal acidosis in lactating dairy cattle. A total of 16 rumen-fistulated, lactating Holstein dairy cattle (618±35 kg of body weight, 221±32 days in milk) were used in a randomized complete block design study. All cattle were … …American Journal of Animal and Veterinary Sciences, Vol. 7, Issue 3 · 19 Sep 2012 · 8,038 accessesView PDFCite