Influence of The Extract of Brown Seaweed (Turbinaria decurrens Bory) on the Histology of Colon on AOM-DSS Mouse Model

Utilization of seaweed as a commodity trading or as a raw materials industry is still relatively small compared with the diversity of seaweed species in Indonesia. Turbinaria decurrens Bory is one of the brown seaweed species that has a high enough fucoxanthin content compared with other brown seaweed species. Fucoxanthin is one of the carotenoid pigments that have long been known to have bioactivity as anti-inflammatory, antioxidant and anticarcinogenic that can neutralize free radicals. Therefore, in this study, we evaluated another function of Turbinaria decurrens Bory as a candidate for colon cancer drug. To this end, we used 36 Male Swiss Webster mice aged 5-6 weeks and divided into six groups. Group I as normal groups, Group II as AOM-DSS induced cancer group, Group III as Aspirin treated group; Group IV-VI Brown Seaweed extracts dose 1 to 3 are 3.61; 7.22; 14.41 mg/kg body weight, respectively. All groups except normal group treated by 12.5 mg/kg body weight of AOM by intraperitoneal injection followed by two cycles of DSS treatment and five days for each treatment. At the end of the experiment, mice sacrificed and the colon was isolated and subject to histology treatment. As a result, showed that AOM-DSS could make colon cancer in all mice. The administration of brown seaweed extract at the dose I could prevent the development of cancer. Increasing dose could not prevent better, but it makes worst.