The Effect of Antioxidants Supplement on Lipid Peroxidation and Serum Aryl Esterase Enzyme in Rheumatoid Arthritis Patients

Problem statement: Rheumatoid Arthritis (RA) is one of the common autoimmune diseases with unknown etiology that its prevalence ratio in females to males is 3:1. It has been suggested that serum antioxidant levels is reduced and lipid peroxidation is increased due to oxidative stress. According to a recent research, antioxidant supplements may play an important role in controlling oxidative stress. The aim of this study is to investigate the effect of antioxidants supplement on serum Aryl Esterase Enzyme (ARE) and serum Malondialdehyde (MDA) in RA patients. Approach: A pre-post study was conducted in a 3 month period on 40 female RA patients which receiving daily one SelenPlus capsule (selenium 50 mcg, zinc 8 mg, vitamin A 400 mcg, vitamin C 125 mg and vitamin E 40 mg). About 5 mL venous blood sample was taken from all participants and serum levels of ARE and MDA were measured spectrophotometerically. Distribution of the variables were assessed using histogram with normal curve as well as Kolmogrov-Smirnov test and Data were analyzed with paired t test for differences between pre-post data using SPSS software version 13.5. Results: Out of 40 patients, 39 completed the study. In comparison with the baseline, we found significant differences between serum aryl esterase and malondialdehyde values before and after intervention. The 3-month supplementation resulted in increasing ARE activity (p<0.001) and reduction of MDA levels (p<0.001). Conclusion: The antioxidantssupplement for 3 months resulted in significant improvement in ARE activity and reduction of lipid peroxidation marker and may be helpful in RA treatment.