Comparison of IncL/M Plasmids Using the Neighbor-Joining Method on Basis repA and excA Genes
Touati Abdelaziz1 & Mairi Assia1 Show affiliations
- Université de Bejaia, Algeria
Current Research in Bioinformatics, Volume 8, Issue 1, pp. 14–17 · doi.org/10.3844/ajbsp.2019.14.17
Abstract
The aim of this study is to make comparison of IncL/M groups on basis of two genes candidates’ repA and excA genes. The sequences of 27 plasmids were compared using the neighbor-joining method. This method was used to construct a phylogenetic tree for the nucleotide sequences of two genes (repA and excA), using the program MEGA X software. The evolutionary distances were computed using the maximum composite likelihood method. The neighbor-joining method analysis showed different results based on the gene used for comparison. The repA gene was more accurate than excA gene to distinguish between different incL/M plasmids. This study suggested that IncL/M plasmids harboring different antibiotic resistance genes have evolved differently.
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Abdelaziz, T. & Assia, M. (2019). Comparison of IncL/M Plasmids Using the Neighbor-Joining Method on Basis repA and excA Genes. Current Research in Bioinformatics, 8(1), 14–17. https://doi.org/10.3844/ajbsp.2019.14.17
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