Sensitization of Photohemolysis by a New Extraction from Flowers and Aerial Parts of Cichorium Pumilum Jacq: Effects of Inulin and Hydrogen Peroxide

Addition of inulin and hydrogen peroxide to new photosensitizers extracted from the flowers and aerial parts of the chicory (Cichorium Pumilum Jacq) have been investigated. Some of the isolated photosensitizers remain unidentified compounds. Chicory extracts are found to be dependent on light and temperature. Higher photohemolysis rate was noticed under the influence of light as compared to temperature. The effect of H₂O₂ on phototoxicity reported in this study showed a rapid reduction in the fractional hemolysis percentage at higher temperature. Compared to erythrocytes with higher concentrations and under the influence of solar irradiance 505.29±24.96w/m², erythrocytes with lower concentrations of inulin and H₂O₂ were ruptured faster. Samples with 0.95 mg/ml of herb extract and different concentrations of inulin (1mg/ml - 2mg/ml) shows no temperature effects at 40°C in the absent of sunlight.