Enhanced Antibacterial Activity of Blended Essential Oils against Staphylococcus Aureus and Methicillin-Resistant Staphylococcus Aureus

We aimed to evaluate the antibacterial effects of four blended and four single essential oils on the gram-positive bacterium Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA). Minimum inhibitory concentration (MIC) testing was performed using disk diffusion and broth microdilution methods according to the Clinical and Laboratory Standards Institute criteria. The blended essential oils exhibited more powerful antibacterial activity than the single oils, and Relaxation Aroma Blending Oil exhibited a stronger inhibitory effect against S. aureus and MRSA than the Bergamot single oil. The average inhibition zones of Relaxation Aroma Blending Oil against S. aureus and MRSA were 19.6 ± 0.2 mm and 18.4 ± 0.2 mm, respectively, indicating that Relaxation Aroma Blending Oil produced inhibition zones that were 30% and 58% larger than those formed by Bergamot single oil against S. aureus and MRSA, respectively. These findings highlight blended essential oils as promising natural antibacterial agents.