In vitro Antioxidant and Cytoprotective Potentials of Postbiotic ProbioMETA G55

Studies have demonstrated the favorable beneficial impact of probiotic metabolites on the skin. However, the use of postbiotics for skincare is a relatively new field despite their high safety and stability, which guarantee a longer shelf life. The postbiotic ProbioMETA G55(G55) is a skincare raw material, but none of the studies have evaluated the antioxidant capacity of G55 in skin care products so far. Thus, this study aimed to investigate the antioxidant activity and cytoprotective effect of G55 using B16-F10 melanoma cells. The antioxidant activity of G55 was determined using the 2,2-diphenyl-1-picrylhydrazyl free radical method. Additionally, the effects of G55 on B16-F10 cytoprotection and cell viability were evaluated using the lactate dehydrogenase and WST-8 assays, respectively. Moreover, cell suspension pH, among other parameters, was analyzed using the i-Smart 300 analyzer. The results showed that G55 exhibited promising antioxidant activity and preserved B16-F10 cell viability. G55 also decreased melanin levels in B16-F10 cells. These findings imply that G55 is a skin-safe substance and antioxidant postbiotic that can be used in anti-aging, whitening, and other cosmetic formulations.