Synthesis and Biological Evaluation of 6-Hydroxy-4-Methyl-5,7-(Bis-p-ChlorophenylAzo) Coumarin

6-hydroxy-4-methyl-5,7-(bis-p-chlorophenylazo)coumarinhas been synthesized  and characterizedby CHN elemental analysis, FTIR, ¹H-NMR-spectroscopy andmass-spectral data. Cytotoxic screening by MTT assay was carried out on the compoundagainst breast cancer cells. The overall results from preliminary screeningprogram revealed that the cell proliferation was highly inhibited by 6-hydroxy-4-methyl-5,7-(bis-p-chlorophenylazo)coumarin with the value of 2.81%, at concentration of 30 µg mL^-1 comparedwith the untreated control cells and also possessed a good chelating activitywith IC₅₀ value 1.87 µg mL^-1. It issuggested that the cytotoxic activity is affected by hydroxyl and halogengroups as these groups have high electron affinity and high electronegativity.