Volume 25, Issue 4 (2025) | OnLine Journal of Biological Sciences

Development and Evaluation of Acaricidal Soap Formulated from Azadirachta indica (Neem) and Carica Papaya (Papaya) Leaf Extracts Using Bio-Assay Guided Approach

Alfredo V. Corpuz — 2025-11-27

OnLine Journal of Biological Sciences, Published online: 27 November 2025; doi:10.3844/ojbsci.2025.833.843

The environment, human health, and animals are all at risk as a result of the pervasive use of chemical-based products to control ticks and lice in animals, mostly dogs. This investigation was designe...

Genetic Testing for Inherited Thrombophilia in Women with Recurrent Pregnancy Loss: Prevalence and Risk Assessment of Factor V Leiden, Prothrombin, and MTHFR Mutations

Lina M. Al Momani — 2025-11-29

OnLine Journal of Biological Sciences, Published online: 29 November 2025; doi:10.3844/ojbsci.2025.833.840

Genetic testing for inherited thrombophilia in women with recurrent pregnancy loss (RPL) may play an important role in detecting high-risk subjects. DNA was extracted from peripheral blood leukocytes....

Quantitative Analysis of Hypothalamic Proline-Rich Peptide-1 in Blood Serum of Patients with Malignant Tumors

Narine Tumasyan — 2025-11-27

OnLine Journal of Biological Sciences, Published online: 27 November 2025; doi:10.3844/ojbsci.2025.844.851

The proline-rich-peptides are a class of neurosecretory hypothalamic bioactive polypeptides derived from cattle and comprise the content of bovine posterior pituitary neurosecretory granules. These pe...

Comparative Phytochemical Analysis of Hydrodistilled Essential Oils of Scots Pine (Pinus Sylvestris L.) Needles From Central Kazakhstan

Kuralay Tuleshova — 2025-11-28

OnLine Journal of Biological Sciences, Published online: 28 November 2025; doi:10.3844/ojbsci.2025.852.859

This study presents a detailed phytochemical investigation of hydrodistilled essential oils extracted from the needles of Pinus sylvestris (Scots pine), collected from fifteen natural populations loca...

Morphological Diversity of Isolated Mycobacterial Cultures in the Republic of Kazakhstan: Implications for Diagnostics and Treatment

Assiya Borsynbayeva — 2025-12-01

OnLine Journal of Biological Sciences, Published online: 1 December 2025; doi:10.3844/ojbsci.2025.860.872

The study of mycobacterial morphology in the Republic of Kazakhstan is relevant due to the increasing burden of tuberculosis and other mycobacterial diseases. The aim of this study was to comprehensiv...

A Review on Ramie (Boehmeria nivea L. Gaudich.): From Textile to Medicine and the Threats of Pests and Diseases

Victor Corbita — 2025-12-02

OnLine Journal of Biological Sciences, Published online: 2 December 2025; doi:10.3844/ojbsci.2025.873.891

Ramie (Boehmeria nivea L. Gaud.), also known as “China grass”, is a perennial herbaceous plant of the Urticaceae family. Ramie is considered one of the ancient and important economic and natural f...

Enhancing Corn Yield and Soil Fertility in Light Chestnut Soils Using BioEcoGum: A Case Study from Kazakhstan

Kuanysh Karabayev — 2026-01-09

OnLine Journal of Biological Sciences, Published online: 9 January 2026; doi:10.3844/ojbsci.2025.892.900

This study evaluates the efficacy of the biological fertilizer BioEcoGum on corn yield and soil fertility under temperate climatic conditions at Karasai, Kazakhstan. The aim of this study was to asses...

Monitoring of Reproductive Capacity of Dairy Cattle in Eurasia: A Comparative Study in Kazakhstan and the Republic of Tuva

Anuarbek Temirbekovich Bissembayev — 2026-01-14

OnLine Journal of Biological Sciences, Published online: 14 January 2026; doi:10.3844/ojbsci.2025.901.910

This study investigates the reproductive capacity of dairy cattle populations in the Republic of Kazakhstan and the Republic of Tuva (Russian Federation). Experimental work was conducted at four study...

Unveiling the Potential of Dombeya buettneri K.Schum As a Drug Agent: Molecular Docking of Compounds With Enzymes Linked to Diabetes and Neurodegenerative Disease

Esther Emem Nwanna — 2026-02-01

OnLine Journal of Biological Sciences, Published online: 1 February 2026; doi:10.3844/ojbsci.2025.911.922

This study investigated the mechanisms of Dombeya buettneri (snowball leafy) extracts on diabetes, hypertension, and neurodegenerative diseases. It compared extracts obtained using steep water (a by-...

Biomolecular Identification of Bacteria Endophytic From Betel Leaf in Jambi City Forest Park Area as Antibiotic-Producing Potential

Arzita — 2026-02-15

OnLine Journal of Biological Sciences, Published online: 15 February 2026; doi:10.3844/ojbsci.2025.923.929

Background and Objective: Muhammad Sabki City Forest Park in Jambi City has three types of betel plants that contain endophytic bacteria. In 2021, six isolates of endophytic bacteria with the potentia...

Decoding Lung Cancer: PLAU as a Critical Driver and Diagnostic Marker

Stanley Cho — 2026-02-15

OnLine Journal of Biological Sciences, Published online: 15 February 2026; doi:10.3844/ojbsci.2025.930.939

Lung cancer remains a leading cause of cancer-related mortality, and understanding its underlying molecular mechanisms is critical for developing targeted therapies. We developed a novel tool called t...

Enhancing Sweet Corn Growth and Yield through the Integrated Application of Bioneensis Biofertilizer and Rice Husk Biochar

Sumihar Hutapea — 2026-02-06

OnLine Journal of Biological Sciences, Published online: 6 February 2026; doi:10.3844/ojbsci.2025.940.955

Sweet corn is an essential agricultural product in Indonesia and contributes to national food security. Consequently, numerous strategies can be implemented to increase the yield of sweet corn, such a...

Decoding Late Blight of Potato and Tomato: Insights into Pathogen Dynamics, Host Interactions and Emerging Solutions

Humayra Ferdus — 2026-02-05

OnLine Journal of Biological Sciences, Published online: 5 February 2026; doi:10.3844/ojbsci.2025.956.984

The oomycete Phytophthora infestans is the source of late blight, a devastating disease that significantly impacts on tomato and potato cultivation worldwide, threatening food security and causing con...

In Vitro and in Silico Evaluation of Bioactive Compounds from Usnea longissima Ach. as Antimicrobial Agents

Oky Kusuma Atni — 2026-02-10

OnLine Journal of Biological Sciences, Published online: 10 February 2026; doi:10.3844/ojbsci.2025.985.999

Lichens, formed from symbiotic association between of fungi with phototrophic organisms, generate a variety of bioactive chemicals having medicinal potential. This research aimed to examine the antiba...

Morphological Changes in Brain Tissue During Experimental Hypoxia

Aizhan Mussayeva — 2026-02-20

OnLine Journal of Biological Sciences, Published online: 20 February 2026; doi:10.3844/ojbsci.2025.1000.1007

The extensive application of hypoxic training to enhance cerebral circulation highlights its significant role in effectively realizing the therapeutic and health-promoting benefits of this method. The...

The Impact of Changes in Forage Vegetation for Saiga (Saiga Tatarica) On Natural Pastures in The Steppe Zone of the West Kazakhstan Region

Amanay Myrzabayev — 2026-02-07

OnLine Journal of Biological Sciences, Published online: 7 February 2026; doi:10.3844/ojbsci.2025.1008.1015

This study investigates the impact of recent climate changes and reduced anthropogenic pressure on the vegetation cover in the steppe regions of Russia and Kazakhstan, particularly focusing on the eff...

Signs of Predisposition to Atherosclerosis in Fetal Development

Anastasia Vladimirovna Poznyak — 2026-02-15

OnLine Journal of Biological Sciences, Published online: 15 February 2026; doi:10.3844/ojbsci.2025.1016.1025

Atherosclerotic Cardiovascular Disease (ASCVD) remains a significant global health concern, contributing to a substantial number of deaths worldwide. The etiology of this condition is multifaceted, in...

Correlation of Atherosclerosis and Osteoarthritis, the Role of Inflammation in the Pathogenesis of Osteoarthritis and Atherosclerosis

Anastasia V. Poznyak — 2026-02-15

OnLine Journal of Biological Sciences, Published online: 15 February 2026; doi:10.3844/ojbsci.2025.1026.1037

Cardiovascular Diseases (CVD) rank among the leading causes of death globally, accounting for a substantial proportion of mortality rates and affecting millions of individuals. Risk factors such as ag...

Potentiometric Biosensor for Highly Toxic Pb (lead) and Cd (cadmium) Detection in Milk Using Layer-by-Layer Immobilization

Zhainagul Kakimova — 2026-02-08

OnLine Journal of Biological Sciences, Published online: 8 February 2026; doi:10.3844/ojbsci.2025.1038.1049

Milk contamination with heavy metals such as lead (Pb) and Cadmium (Cd) poses a serious threat to public health, particularly in industrial regions. To address the need for rapid and on-site detection...

Genetic and Environmental Interactions in Spring Wheat Resistance to Bipolaris Sorokiniana Root Rot in Kazakhstan

Yerlan Dutbayev — 2026-03-07

OnLine Journal of Biological Sciences, Published online: 7 March 2026; doi:10.3844/ojbsci.2025.1050.1063

Common root rot caused by Bipolaris sorokiniana represents a major constraint to wheat production in Kazakhstan. This study evaluated the resistance of 30 spring bread and durum wheat genotypes under ...

Impact of Agritechnologies on the Biomass and Nutritional Parameters of Crops: A Study of Corn, Soybean, and Pea in Southeast Kazakhstan

Gulvira Yessenbayeva — 2026-02-16

OnLine Journal of Biological Sciences, Published online: 16 February 2026; doi:10.3844/ojbsci.2025.1063.1069

The study investigates the impact of various agritechnologies on the biomass and nutritional parameters of corn, soybean, and winter pea cultivated in Southeast Kazakhstan. This research is particular...

Unraveling NETosis: From Immune Mechanisms to Therapeutic Innovations

Anastasia V. Poznyak — 2026-02-21

OnLine Journal of Biological Sciences, Published online: 21 February 2026; doi:10.3844/ojbsci.2025.1070.1082

Neutrophil Extracellular Traps (NETs) are integral to immune responses, serving both protective and pathological roles in inflammatory diseases. Despite increasing recognition of NETs, significant gap...

Species Diversity of Lichens in the Burabay State National Park, Kazakhstan

Aizhan Baubekova — 2026-02-16

OnLine Journal of Biological Sciences, Published online: 16 February 2026; doi:10.3844/ojbsci.2025.1083.1089

Lichens constitute essential components of terrestrial ecosystems, and comprehensive documentation of lichen diversity is fundamental for conservation and ecosystem management. This study investigated...

Cloning and Expression of SCAMP3 in Transgenic Silkworm (Bombyx mori)

Selly S. Rajagukguk — 2026-02-16

OnLine Journal of Biological Sciences, Published online: 16 February 2026; doi:10.3844/ojbsci.2025.1090.1098

SCAMP3 (Secretory Carrier Membrane Protein 3) is a crucial component of vesicular trafficking pathways and plays important roles in endocytosis and protein recycling. Previous studies demonstrate SCAM...

Molecular Identification and Antioxidant Profiling of Penicillium heteringtonii EUA-017 from Mangrove (Avicennia alba) in West Sumatra, Indonesia

Anthoni Agustien — 2026-03-25

OnLine Journal of Biological Sciences, Published online: 25 March 2026; doi:10.3844/ojbsci.2025.1099.1109

Mangrove ecosystems harbor diverse endophytic fungi that produce bioactive secondary metabolites with potential antioxidant activities. This study aimed to identify an endophytic fungal isolate (EUA-0...