Volume 10, Issue 1 (2014) | American Journal of Biochemistry and Biotechnology

OPTIMIZATION OF ALKALINE PROTEASE PRODUCTION BY STREPTOMYCES AMBOFACIENS IN FREE AND IMMOBILIZED FORM

Nayera A.M. Abdelwahed — 2013-12-28

American Journal of Biochemistry and Biotechnology, Published online: 28 December 2013; doi:10.3844/ajbbsp.2014.1.13

Optimization of alkaline protease production by Streptomyces ambofaciens NRRL 2420 in free and immobilized form was investigated using submerged fermentation technique. The optimum conditions for maxi...

CELL DETACHMENT BY PROLYL-SPECIFIC ENDOPEPTIDASE FROM WOLFIPORIA COCOS

Nicole Mika — 2014-02-08

American Journal of Biochemistry and Biotechnology, Published online: 8 February 2014; doi:10.3844/ajbbsp.2014.14.21

As requirements for Advanced Therapy Medicinal Product (ATMP) production differ from other production processes (e.g., therapeutic protein production), cell detachment is often a crucial step for the ...

LANGMUIR-BLODGETT NANOTEMPLATE CRYSTALLIZATION COMBINED TO LASER-MICROFRAGMENTATION UNIQUELY CHARACTERIZE PROTEINS CRYSTALS BY SYNCHROTRON MICRODIFFRACTION

Claudio Nicolini — 2014-02-11

American Journal of Biochemistry and Biotechnology, Published online: 11 February 2014; doi:10.3844/ajbbsp.2014.22.30

Laser-induced microfragmentation of LB nanotemplate-induced protein crystals in glycerol solution results in distinct, coherently diffracting domains. Only crystals produced according to the Langmuir-...

SCANNING ELECTRON MICROSCOPE IMAGING OF AMYLOID FIBRILS

Eisuke Takai — 2014-03-01

American Journal of Biochemistry and Biotechnology, Published online: 1 March 2014; doi:10.3844/ajbbsp.2014.31.39

This study demonstrated the applicability of Scanning Electron Microscopy (SEM) for the observation of amyloid fibrils without staining. As model specimens, two types of amyloid fibrils with different...

A SHORT INTERFERING RNA MOLECULAR BEACON FOR THE ATTENUATION OF MYCOBACTERIAL INFECTION

Remo George — 2014-03-04

American Journal of Biochemistry and Biotechnology, Published online: 4 March 2014; doi:10.3844/ajbbsp.2014.40.49

The ability of the pathogen Mycobacterium Tuberculosis (MTB) to invade and survive within macrophages of granulomas is attributed to the product of the Mammalian Cell Entry (MCE) operon whose gene, mc...

EXERCISE TRAINING REVERSES THE DELETERIOUS EFFECT OF SUCROSE INTAKE ON INSULIN RESISTANCE AND VISCERAL FAT MASS DEPOSITION ON MICE

Julio Cesar Mendes Soares — 2014-03-28

American Journal of Biochemistry and Biotechnology, Published online: 28 March 2014; doi:10.3844/ajbbsp.2014.50.57

Abnormal fat deposition has been identified as an important mechanism of obesity-associated with insulin resistance. Literature data also indicate that exercise can improve insulin action. The heme pa...

ISOLATION AND DETECTION OF HELICOBACTER PYLORI FROM PATIENTS SUFFERING FROM PEPTIC ULCER USING BIOCHEMICAL TESTS AND MOLECULAR TECHNIQUES

Ruba S. Abu-Sbeih — 2014-03-28

American Journal of Biochemistry and Biotechnology, Published online: 28 March 2014; doi:10.3844/ajbbsp.2014.58.68

Helicobacter pylori is the causative agent of most cases of gastritis. There is no established gold standard for the diagnosis of H. pylori infection. A reliable diagnosis is crucial to confirm that e...

COMPARATIVE ANALYSIS OF INTER SIMPLE SEQUENCE REPEATS AND SIMPLE SEQUENCE REPEATS MARKERS: GENETIC ANALYSIS OF DESCHAMPSIA CESPITOSA POPULATIONS GROWING IN METAL CONTAMINATED REGIONS IN CANADA

K.K. Nkongolo — 2014-04-08

American Journal of Biochemistry and Biotechnology, Published online: 8 April 2014; doi:10.3844/ajbbsp.2014.69.80

Comparative studies conducted on the genetic variation of metal-tolerant populations and their non-metal-tolerant counterparts have been performed on numerous species using isozyme markers. Analysis o...

DEVELOPMENT OF A BACTERIAL CULTURE SYSTEM USING A PAPER PLATFORM TO ACCOMMODATE MEDIA AND AN INK-JET PRINTING TO DISPENSE BACTERIA

Tithimanan Srimongkon — 2014-04-26

American Journal of Biochemistry and Biotechnology, Published online: 26 April 2014; doi:10.3844/ajbbsp.2014.81.87

Generally, bacterial culture is performed manually and is subject to error. Here, we created a novel, well-ordered and reliable system for dispensing bacteria microscopically by using paper and an ink...

ASSESSING THE GENETIC RELATIONSHIP OF TAIF ROSE WITH SOME ROSE GENOTYPES (ROSA SP.) BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA, INTER SIMPLE SEQUENCE REPEAT AND SIMPLE SEQUENCE REPEAT MARKERS

Salah El-Din El-Assal — 2014-04-29

American Journal of Biochemistry and Biotechnology, Published online: 29 April 2014; doi:10.3844/ajbbsp.2014.88.98

Taif-roses are a famous rose type that cultivated in Taif region and well known with their deep and intensive fragrance in the Arabian World. Despite of the great economical importance of Taif-roses f...