Volume 4, Issue 4 (2008) | American Journal of Biochemistry and Biotechnology

The Effect of Molecular Chaperone, Alpha-Crystallin, on the Heat-Induced Aggregation of Beta-lactoglobulin

Arezou Ghahghaei — 2008-12-31

American Journal of Biochemistry and Biotechnology, Published online: 31 December 2008; doi:10.3844/ajbbsp.2008.317.324

Aggregation of beta-lactoglobulin occurs mainly via intermolecular disulphide bond exchange. Upon heating, beta-lactoglobulin aggregated which increased with increasing pH. The presence of DTT led to ...

Plantlet Production through Development of Competent Multiple Meristem Cultures from Male Inflorescence of Banana, Musa acuminta cv. 'Pisang Mas' (AA)

S. Wirakarnain — 2008-12-31

American Journal of Biochemistry and Biotechnology, Published online: 31 December 2008; doi:10.3844/ajbbsp.2008.325.328

A protocol was developed for the establishment of competent multiple meristem cultures of banana, Musa acuminta cv. 'Pisang Mas' (AA) derived from male inflorescence. The white floral merist...

Effect of Stages of Maturity and Ripening Conditions on the Physical Characteristics of Tomato

K.M. Moneruzzaman — 2008-12-31

American Journal of Biochemistry and Biotechnology, Published online: 31 December 2008; doi:10.3844/ajbbsp.2008.329.335

The study was carried out to undertake the effect of different stages of maturity and ripening conditions on the physical characteristics of tomato fruit during the process of ripening. Fruit of three...

Effect of Stages of Maturity and Ripening Conditions on the Biochemical Characteristics of Tomato

K.M. Moneruzzaman — 2008-12-31

American Journal of Biochemistry and Biotechnology, Published online: 31 December 2008; doi:10.3844/ajbbsp.2008.336.344

An experiment was carried out to evaluate the biochemical characteristics of tomato in different maturity stages and ripening conditions. Tomato (Lycopersicon esculentum Mill) fruits (cv. Ruma VF) wer...

A Review on Supercritical Fluid Extraction as New Analytical Method

K. A. Abbas — 2008-12-31

American Journal of Biochemistry and Biotechnology, Published online: 31 December 2008; doi:10.3844/ajbbsp.2008.345.353

This review study summarized the Supercritical Fluid Extraction (SFE) as new analytical method. The advantage and disadvantage of CO2 (SC-CO2 )as well as special applications of SFE in food processing...

Perspectives of Solid State Fermentation for Production of Food Enzymes

Cristobal Noe Aguilar — 2008-12-31

American Journal of Biochemistry and Biotechnology, Published online: 31 December 2008; doi:10.3844/ajbbsp.2008.354.366

Food industry represents one of the economic sectors where microbial metabolites have found a wide variety of applications. This is the case of some enzymes, such as amylases, cellulases, pectinases a...

Identification of Major Cultivable Aerobic Bacteria in the Oral Cavity of Malaysian Subjects

Koshy Philip — 2008-12-31

American Journal of Biochemistry and Biotechnology, Published online: 31 December 2008; doi:10.3844/ajbbsp.2008.367.370

Culture dependent and culture independent methods have shown that about 600 species of bacteria inhabit the human oral cavity. While some oral microorganisms have a direct link to dental caries, perio...

Modification of Artificial Oliogosaccharides in Recombinant Escherichia coli Cells

Tomohisa Kato — 2008-12-31

American Journal of Biochemistry and Biotechnology, Published online: 31 December 2008; doi:10.3844/ajbbsp.2008.371.374

Artificial oligosaccharides were modified using recombinant Escherichia coli cells that overexpress sialidase. Based on the principle of the saccharide primer method by using bacterial cells overexpre...

Improving Protein 3D Structure Prediction Accuracy using Dense Regions Areas of Secondary Structures in the Contact Map

Amer F. Al-Badarneh — 2008-12-31

American Journal of Biochemistry and Biotechnology, Published online: 31 December 2008; doi:10.3844/ajbbsp.2008.375.384

Problem Statement: The protein folding problem is a fundamental problem in structural molecular biology. This problem describes how a protein is transformed from its primary sequence (i.e., amino ...

An Efficient Production of Oligosaccharides by a Reaction using Whole Mammalian Cells as Biocatalysts

Tomohisa Kato — 2008-12-31

American Journal of Biochemistry and Biotechnology, Published online: 31 December 2008; doi:10.3844/ajbbsp.2008.385.392

Problem statement: Mammalian cells were used for the production of oligosaccharides by saccharide primer method. However, because cells in culture are used, productivity of oligosaccharides is low. Ap...

Effect of Nitrogen Source and Carbon to Nitrogen Ratio on Hydrogen Production using C. acetobutylicum

Mohd Sahaid Kalil — 2009-12-31

American Journal of Biochemistry and Biotechnology, Published online: 31 December 2009; doi:10.3844/ajbbsp.2008.393.401

Problem statement: One of the main factors influenced the bacterial productivity and total yield of hydrogen is the nitrogen source and its concentration. Approach: Using different nitrogen source wit...

Antibacterial and Antioxidant Activities of Typhonium Flagelliforme (Lodd.) Blume Tuber

Syam Mohan — 2008-12-31

American Journal of Biochemistry and Biotechnology, Published online: 31 December 2008; doi:10.3844/ajbbsp.2008.402.407

Problem Statement: Multiple drug resistance in human pathogenic micro organisms has developed due to indiscriminate use of modern antimicrobial drugs generally used in the management of infectious dis...

Investigations in the molecular events of Transitional Cell Carcinoma of the Bladder

H.S. Kadhim — 2008-12-31

American Journal of Biochemistry and Biotechnology, Published online: 31 December 2008; doi:10.3844/ajbbsp.2008.408.415

Problem Statement: Transitional Cell Carcinoma (TCC) of the bladder is a significant health problem worldwide. The molecular mechanisms of tumor development and progression are complicated but lik...

A Review on Correlations between Fish Freshness and pH during Cold Storage

K.A. Abbas — 2008-12-31

American Journal of Biochemistry and Biotechnology, Published online: 31 December 2008; doi:10.3844/ajbbsp.2008.416.421

Freshness of fish in most markets was found to be the significant quality parameter. The state of fish freshness was assessed by various methods but most of them were costly, time-consuming and not us...

An Optimized Medium for Screening of L-Asparaginase production by Escherichia coli

Younes Ghasemi — 2008-12-31

American Journal of Biochemistry and Biotechnology, Published online: 31 December 2008; doi:10.3844/ajbbsp.2008.422.424

Purified L-asparaginase II from Escherichia coli has been supplied and employed in the acute leukemia and other malignant neoplasms chemotherapy. L-asparaginase II gene (ansB) in E. coli is under regu...

Serotonergic Properties of the Roots of Clerodendron capitatum

Siddig Ibrahim Abdel Wahab — 2008-12-31

American Journal of Biochemistry and Biotechnology, Published online: 31 December 2008; doi:10.3844/ajbbsp.2008.425.430

The aim of this study is to investigate the pharmacological effects of the methanolic extract of Clerodendron capitatum, CC, (family verbenaceae). These investigations were carried out on isolated fro...

Perception of Extension Specialists about the Role of Extension in the Production and Adoption of the Genetically Modified Crops in Iran

Seyed Jamal F. Hosseini — 2008-12-31

American Journal of Biochemistry and Biotechnology, Published online: 31 December 2008; doi:10.3844/ajbbsp.2008.431.437

Extension specialists were surveyed in order to explore their perception about the role of extension in the production and adoption of Genetically Modified (GM) crops in Iran. The methodology used in ...

Gastroprotective Effects of Melastoma malabathricum Aqueous Leaf Extract against Ethanol-Induced Gastric Ulcer Iin Rats

Fouad Hussain — 2008-12-31

American Journal of Biochemistry and Biotechnology, Published online: 31 December 2008; doi:10.3844/ajbbsp.2008.438.441

The gastro-protective effects of the aqueous leaf extract of Melastoma malabathricum was investigated against ethanol-induced gastric mucosal injuries in rats. There were four groups of adult sprague ...

Protective Effects of Covalent Cross-Linking on Proteolysis of Human Coproporphyrinogen Oxidase and Implications for Porphyria

Ahmed W. Jafri — 2008-12-31

American Journal of Biochemistry and Biotechnology, Published online: 31 December 2008; doi:10.3844/ajbbsp.2008.442.449

The effects of covalent cross-linkers on the enzyme, coproporphyrinogen oxidase, had been previously studied but their role in protecting the enzyme from protease cleavage has not been evaluated. Ther...