Volume 15, Issue 4 (2019) | American Journal of Biochemistry and Biotechnology

Optimization for the Extraction of Polysaccharide from Walnut (Juglans regia L.) Leaves: Antioxidant Activities in Vitro

Shuqing Yang — 2019-10-23

American Journal of Biochemistry and Biotechnology, Published online: 23 October 2019; doi:10.3844/ajbbsp.2019.163.178

Cellulase-assisted extraction of Walnut Leaf Polysaccharide (WLP) was studied. Single factor test and response surface design were employed to optimize the technological conditions. The consequences i...

Inhibition Effect of Walnut Leaf Polysaccharide on HepG2 Cells in vitro

Shuqing Yang — 2019-10-29

American Journal of Biochemistry and Biotechnology, Published online: 29 October 2019; doi:10.3844/ajbbsp.2019.179.189

In this study, the effect of Walnut Leaf Polysaccharides (WLP) on human liver tumor HepG2 cells was investigated. The results showed that WLP had dose-dependent and time-dependent inhibitory effects o...

Enhancement Effect of BAP and Spent Mushroom Compost in Micro-Propagation of Sabah Snake Grass

Wan Nurul Hidayah Wan Anuar — 2019-10-31

American Journal of Biochemistry and Biotechnology, Published online: 31 October 2019; doi:10.3844/ajbbsp.2019.190.197

Medicinal plants which is Clinacantus nutans (C. nutans) that known as Sabah Snake Grass or Belalai Gajah can treat several diseases including cancer. In order to provide and grow this plant in large ...

The Use of α-MF Signal Peptide Without Spacer for Producing Insulin Aspart Precursor in Pichia pastoris KM71

Popi Asri Kurniatin — 2019-10-31

American Journal of Biochemistry and Biotechnology, Published online: 31 October 2019; doi:10.3844/ajbbsp.2019.198.207

A spacer peptide (Glu-Ala repeats) connects N-terminal of a polypeptide precursor to the signal peptide of α-mating factor (α-MF) from Saccharomyces cerevisiae. During the polypeptide precursor secr...

Two Co-Expression Strategies for Eicosapentaenoic Acid Production in Mortierella alpina

Xin Tang — 2019-12-05

American Journal of Biochemistry and Biotechnology, Published online: 5 December 2019; doi:10.3844/ajbbsp.2019.208.217

The omega-3 long-chain Polyunsaturated Fatty Acid (PUFA) Eicosapentaenoic Acid (EPA) has beneficial effects on human health, leading to its use in health products and foods. The oleaginous microorgani...

Production and Characterization of Hydrocarbon Degrading Peroxidase from Rhizopus and Saccharomyces Species

Ferdinand U. Paschaline — 2019-12-07

American Journal of Biochemistry and Biotechnology, Published online: 7 December 2019; doi:10.3844/ajbbsp.2019.218.229

The production, characterization and partial purification of hydrocarbon degrading peroxidase from di-culture of Rhizopus and Saccharomyces species was assessed. The fungi were isolated from hydrocarb...

Nondestructive Assessment of Egg Freshness using a Synchronous Fluorescence Spectral Technique

Jianhu Wu — 2019-12-19

American Journal of Biochemistry and Biotechnology, Published online: 19 December 2019; doi:10.3844/ajbbsp.2019.230.240

Freshness is an important index of egg quality. In this study, a synchronous fluorescence spectral technique was employed to determine the freshness of an intact egg. Synchronous fluorescence spectra ...

Recognizing Dissolved Organic Matter with an Improved Fluorescence Regional Integral Method

Zhang Jianguo — 2019-12-24

American Journal of Biochemistry and Biotechnology, Published online: 24 December 2019; doi:10.3844/ajbbsp.2019.241.250

The aim of this paper was to study transformations of Dissolved Organic Matter (DOM) in marl rock groundwater. To achieve this, we carried out a series of soil column experiments, using a DOM solution...

Isolation and Characterization of Chemical Constituents, Cytotoxicity, Antibacterial and Antioxidant Activity of the Compounds from Leptadenia hastata Roots Extract By

Isaac John Umaru — 2019-12-24

American Journal of Biochemistry and Biotechnology, Published online: 24 December 2019; doi:10.3844/ajbbsp.2019.251.269

The aim of this work was to extract, isolate and characterise the chemical constituent from Leptadenia hastata roots. Fresh roots were washed and dried and was subjected to sequential maceration in po...

The Phytol-rich Essential Oil from Fresh Medicago hispida Exerts Significant Inhibitory Activity against Escherichia coli

Lixue Zheng — 2019-12-28

American Journal of Biochemistry and Biotechnology, Published online: 28 December 2019; doi:10.3844/ajbbsp.2019.270.274

Medicago hispida is one of the most widely distributed and commonly cultivated plants of the genus Medicago in China. It is mainly consumed as a vegetable. In this paper, the extraction, chemical comp...