Volume 1, Issue 2 (2005) | American Journal of Biochemistry and Biotechnology

PDX-1 and MafA: Key Transcription Factors in Pancreas

Hideaki Kaneto — 2005-06-30

American Journal of Biochemistry and Biotechnology, Published online: 30 June 2005; doi:10.3844/ajbbsp.2005.54.63

Pancreatic and duodenal homeobox factor-1 (PDX-1) plays a crucial role in pancreas development, β-cell differentiation and in maintaining normal β-cell function by regulating several β-cell-related...

The Effects of Chinese Traditional Processing Method on Components in Semen Sinapis Albae

Lifang Liu — 2005-06-30

American Journal of Biochemistry and Biotechnology, Published online: 30 June 2005; doi:10.3844/ajbbsp.2005.64.68

Techniques of infrared (IR) spectrophotometry, High Performance Liquid Chromatography (HPLC) and HPLC-mass spectrometry are employed in this study to reveal the changes of the components in Semen sina...

Expression, Purification and Activity Assay of Two New Recombinant Antagonists of Fibrinogen Receptor

Jianbo Yang — 2005-06-30

American Journal of Biochemistry and Biotechnology, Published online: 30 June 2005; doi:10.3844/ajbbsp.2005.69.73

The gene sequence of Decorsin which is extracted from a kind of North American leeches was synthesized. Two recombinant proteins, Annexin V plus Decorsin (AnnV-D39) and Annexin V plus the carboxyl ter...

Application of RNA-DNA Duplex Base Triplets to Antisense Drugs

Jie Yang — 2005-06-30

American Journal of Biochemistry and Biotechnology, Published online: 30 June 2005; doi:10.3844/ajbbsp.2005.74.84

Sixty-four sets of three-dimensional models of RNA-DNA duplex base triplets were constructed based on codons by homology modeling method using software InsightII on Indigo workstation, which should ...

Biosensor Based on Xanthine Oxidase for Monitoring Hypoxanthine in Fish Meat

Helena S. Nakatani — 2005-06-30

American Journal of Biochemistry and Biotechnology, Published online: 30 June 2005; doi:10.3844/ajbbsp.2005.85.89

An amperometric enzyme electrode for the detection of hypoxanthine in fish meat is described. The hypoxanthine sensor was prepared from xanthine oxidase immobilized by covalent binding with glutaralde...

Oxidized Low-density Lipoprotein Upregulates GM2 Activator Protein Gene Expression

Hidekatsu Yanai — 2005-06-30

American Journal of Biochemistry and Biotechnology, Published online: 30 June 2005; doi:10.3844/ajbbsp.2005.90.94

Oxidized low-density lipoprotein (LDL) has been shown to be a powerful regulator of gene expression in monocyte-derived macrophage. To determine the effects of oxidized LDL on macrophage gene expressi...

Imatinib Mesylate Inhibits Glucose Uptake in Gastrointestinal Stromal Tumor Cells by Downregulation of the Glucose Transporters Recruitment to the Plasma Membrane

Hans Prenen — 2005-06-30

American Journal of Biochemistry and Biotechnology, Published online: 30 June 2005; doi:10.3844/ajbbsp.2005.95.102

Imatinib mesylate, the inhibitor of the KIT protein tyrosine kinase that is constitutively activated in Gastrointestinal Stromal Tumors (GISTs), has been established as the first highly effective drug...

Use of Cross-linking to Assess Subunit Interaction of Recombinant Human Coproporphyrinogen Oxidase

Jason R. Stephenson — 2005-06-30

American Journal of Biochemistry and Biotechnology, Published online: 30 June 2005; doi:10.3844/ajbbsp.2005.103.106

To provide further evidence for a dimeric form of coproporphyrinogen oxidase reported using the conventional hydrodynamic methods, bifunctional cross-linkers were incubated with purified, recombinant ...

Effect of UV-C on Drought Tolerance of Alfalfa (Medicago sativa) Callus

Ali Akbar Ehsanpour — 2005-06-30

American Journal of Biochemistry and Biotechnology, Published online: 30 June 2005; doi:10.3844/ajbbsp.2005.107.110

Water stress and unfavorable climate decrease the growth and development of plant globally. Medicago is a valuable plant as a source of food for animal, foliage and for medicine. Since, Iran is locate...

Gender Differences in Baseline Levels of Vascular Endothelial Growth Factor in the Plasma of Alaskan Sled Dogs

Scott W. Kemp — 2005-06-30

American Journal of Biochemistry and Biotechnology, Published online: 30 June 2005; doi:10.3844/ajbbsp.2005.111.114

Angiogenesis and vasculogenesis are two very important processes in the development and maintenance of mammalian health. In sled dogs, the angiogenic role is to supply and support tissue with ample va...

A New Method to Estimate Intrinsic Parameters in the Ping-pong Bisustrate Kinetic: Application to the Oxipolymerization of Phenol

José L. Gómez — 2005-06-30

American Journal of Biochemistry and Biotechnology, Published online: 30 June 2005; doi:10.3844/ajbbsp.2005.115.120

A new method for determining the intrinsic parameters of reaction in processes involving a high initial rate has been developed. The usefulness of this alternative, which consists of determining sever...