Volume 14, Issue 2 (2018) | American Journal of Biochemistry and Biotechnology

Improvement on Extracellular Production of Recombinant Burkholderia cepacia Lu10-1 Lipase by Escherichia coli

Yao Zhang — 2018-05-30

American Journal of Biochemistry and Biotechnology, Published online: 30 May 2018; doi:10.3844/ajbbsp.2018.76.87

The purpose of this study was to investigate several strategies on enhancing extracellular production of recombinant lipase from Burkholderia cepacia Lu10-1 in recombinant Escherichia coli BL21(DE3). ...

Statistical Improvement of Batch Culture with Immobilized Pichia pastoris Cells for rPOXA 1B Laccase Production

Michelle Gouzy-Olmos — 2018-04-26

American Journal of Biochemistry and Biotechnology, Published online: 26 April 2018; doi:10.3844/ajbbsp.2018.88.107

Immobilized Pichia pastoris X33/pGAPZαA-LaccPost-Stop in Ca2+ alginate beads was employed for Pleurotus ostreatus rPOXA 1B laccase batch production. Sequential statistical improvement was achieved th...

Current Laboratory Biosecurity for Handling Pathogenic Viruses

I Made Artika — 2018-04-06

American Journal of Biochemistry and Biotechnology, Published online: 6 April 2018; doi:10.3844/ajbbsp.2018.108.116

The recent emergence of lethal viruses such as Ebola raises some concern about the possibility of the viruses being used as biological weapons. The application of pathogenic viruses as biothreat agent...

Sensitive and Rapid Detection of Sulfate-Reducing Bacteria in Jet Fuel by Loop-Mediated Isothermal Amplification Combined with Lateral Flow Dipstick

Zezhen Li — 2018-04-11

American Journal of Biochemistry and Biotechnology, Published online: 11 April 2018; doi:10.3844/ajbbsp.2018.117.123

The existence of Sulfate-Reducing Bacteria (SRB) is one of the significant reasons for the Microbially Influenced Corrosion (MIC) of jet fuel. Especially for the jet fuel stored by military, since jet...

Improvements on Semi Critical Assisted-Solvent Extraction of Biocrude Oil from Spirogyra. sp Macro Algae

Tulio Chavez-Gil — 2018-04-18

American Journal of Biochemistry and Biotechnology, Published online: 18 April 2018; doi:10.3844/ajbbsp.2018.124.136

Here, we report on an improved chemical method to extract hydrocarbons (CH) and Fatty Acid Methyl Esters (FAME) from wild green macro algae, which is higher than that carried out with conventional Sox...

Space Distribution of Highland Barley GNS and its Relationship with Environmental Factors in the Qinghai-Tibet Plateau

Xibo Feng — 2018-04-26

American Journal of Biochemistry and Biotechnology, Published online: 26 April 2018; doi:10.3844/ajbbsp.2018.137.144

In order to study the space distribution of Grain Number per spike (GNS) and its relationship with the environmental factors in the Qinghai-Tibet Plateau, 83 data samples from different ecological are...

Construction of Recombinant Bioleaching Bacteria Thiobacillus ferrooxidans with Fluoride-Resistant Activity

Yun-Chang Cao — 2018-05-23

American Journal of Biochemistry and Biotechnology, Published online: 23 May 2018; doi:10.3844/ajbbsp.2018.145.153

Thiobacillus ferrooxidans is an important bioleaching bacterium, widely used in leaching, recovery of low-grade metals and environmental bioremediation. In bio-metallurgy industry, fluoride existing i...

The Evolution Force of Genome Reduction in Carnivorous Plants

Ming-Yue Ma — 2018-05-26

American Journal of Biochemistry and Biotechnology, Published online: 26 May 2018; doi:10.3844/ajbbsp.2018.154.161

The introns are widely present in the genome of eukaryotes and the distribution of intron varies greatly among different organisms or different genes. Generally, introns loss is an important way for u...

The Contamination Degree of Characteristic Fungi in Jet Fuel Detected by ATP Bioluminescence

Zezhen Li — 2018-05-28

American Journal of Biochemistry and Biotechnology, Published online: 28 May 2018; doi:10.3844/ajbbsp.2018.162.167

Adenosine Triphosphate (ATP) lysate of the characteristic fungi in jet fuel was screened and three fluorescein-luciferase systems were compared in the paper. The correlation between ATP bioluminescenc...

The Influence of Partial Substitution of Potassium by Sodium on the Growth Effect of Sugarcane

Chao Zheng — 2018-06-12

American Journal of Biochemistry and Biotechnology, Published online: 12 June 2018; doi:10.3844/ajbbsp.2018.168.174

The effects of different potassium and sodium levels on the growth of Taisugar No. 22 (ROC22) were studied by potted nutrient solution-sand culture experiment in this paper. The results showed that un...

Production of Selenium-Enriched Yeast (Kluyveromyces marxianus) Biomass in a whey-based Culture Medium

Nicolás Gurdo — 2018-06-28

American Journal of Biochemistry and Biotechnology, Published online: 28 June 2018; doi:10.3844/ajbbsp.2018.175.182

Two important aspects of agriculture intensification are the reduction in the concentration of specific soil minerals that affects livestock production and the increase of agricultural by-products, wh...