Volume 1, Issue 1 (2005) | American Journal of Biochemistry and Biotechnology

Production of (E) 10-Hydroxy-8-octadecenoic Acid with Lyophilized Microbial Cells

A. Bódalo — 2005-03-31

American Journal of Biochemistry and Biotechnology, Published online: 31 March 2005; doi:10.3844/ajbbsp.2005.1.4

Lyophilized non-proliferating microbial cells of Pseudomonas sp. 42A2 showed their ability to catalyze the bioconversion of oleic acid into (E)-10-hydroxy-8-octadecenoic acid (MHOD) and (E)- 7,10-di...

Evaluation of Transcript Labeling Techniques and Development of a Membrane-based Parallel Gene Expression Assay

Mark A. Fisher — 2005-03-31

American Journal of Biochemistry and Biotechnology, Published online: 31 March 2005; doi:10.3844/ajbbsp.2005.5.12

An inexpensive alternative to micro arrays was developed to examine the expression of small sets of genes in parallel and used to compare direct and indirect transcript labeling methods. Psoralen-biot...

Physics Models of Pronuclear Movements in Eggs

Kang Cheng — 2005-03-31

American Journal of Biochemistry and Biotechnology, Published online: 31 March 2005; doi:10.3844/ajbbsp.2005.13.16

Based on published experimental data, Newton's laws, and Coulomb's law, we investigate natural and normal pronuclear movements in wild types of eggs and develop physics models to fit the experimental ...

Purification of A Recombinant Thrombin-like Enzyme, Gloshedobin by Egg Yolk Antibody-Coupled Adsorbents

Qing Yang — 2005-03-31

American Journal of Biochemistry and Biotechnology, Published online: 31 March 2005; doi:10.3844/ajbbsp.2005.17.21

The gloshedobin, a snake venom thrombin-like enzyme was biosynthesized in the soluble form and purified by egg yolk antibody-coupled adsorbents from E. coli. As His-tag is not favored from the point o...

Biological Control of Fusarium oxysporum f. sp. melonis, the Causal Agent of Root Rot Disease of Greenhouse Cucurbits in Kerman Province of Iran

Sara Shafii Bafti — 2005-03-31

American Journal of Biochemistry and Biotechnology, Published online: 31 March 2005; doi:10.3844/ajbbsp.2005.22.26

Antagonistic activity of 178 soil actinomycete isolates was assayed against Fusarium oxysporum f. sp. melonis Schlecht, Emend (Snyde and Hansen) cause of root rot and fusarium wilt of greenhouse cucur...

Antioxidant, Free Radical Scavenging and Metal Chelating Characteristics of Propolis

Hikmet Geckil — 2005-03-31

American Journal of Biochemistry and Biotechnology, Published online: 31 March 2005; doi:10.3844/ajbbsp.2005.27.31

This study was undertaken to determine the reducing characteristics, metal chelating capability, anti-lipid peroxidative and antiradical properties of propolis compared to two widely used artificial a...

Conformational Analysis of Trans-3, 6-Dibutanal-1, 2, 4, 5-Tetroxane

Eduardo A. Castro — 2005-03-31

American Journal of Biochemistry and Biotechnology, Published online: 31 March 2005; doi:10.3844/ajbbsp.2005.32.36

We report the results of theoretical semiempirical AM1 and PM3 molecular orbital methods on the conformational analysis of the title compound. The relative stability of different isomers and conformer...

Correlation between Tissue and Released VEGF Levels in Urine of Bladder Cancer Patients

Menha Swellam — 2005-03-31

American Journal of Biochemistry and Biotechnology, Published online: 31 March 2005; doi:10.3844/ajbbsp.2005.37.42

Purpose. Angiogenesis is a complex multistep process essential for tumor growth. Vascular endothelial growth factor (VEGF) is a potent endothelial cell mitogen and vascular permeability-inducing agent...

Hydrogen Peroxide Biosensor Based on the Direct Electrochemistry of Myoglobin Immobilized in Poly-3-Hydroxybutyrate Film

Xiang Ma — 2005-03-31

American Journal of Biochemistry and Biotechnology, Published online: 31 March 2005; doi:10.3844/ajbbsp.2005.43.46

Direct electrochemistry of myoglobin (Mb) was observed in a stable film composed of a natural lipid polymer (poly-3-hydroxybutyrate) and Mb, the film of which was modified on a pyrolytic graphite elec...

Synthesis of Sodium Salt of 3,6-Dibutanoic-1,2,4,5-Tetroxane

Eduardo A. Castro — 2005-03-31

American Journal of Biochemistry and Biotechnology, Published online: 31 March 2005; doi:10.3844/ajbbsp.2005.47.49

We report the experimental preparation of the sodium salt of 3,6-dibutanoic-1,2,4,5- tetroxane by addition of saturate solution of sodium hydroxide to the 3,6-dibutanoic-1,2,4,5-tetroxane, synthesized...

Antibacterial Effect of Untreated and Treated (Decolorized) Dyes by Agricultural Wastes

Sibel Kahraman — 2005-03-31

American Journal of Biochemistry and Biotechnology, Published online: 31 March 2005; doi:10.3844/ajbbsp.2005.50.53

Toxicity (antibacterial effect) of untreated and treated (decolorized) dyes on a soil bacterium, Pseudomonas aeruginosa was determined. Low cost and easily available two agricultural residues (cotton ...